Comparative interference by haemolysis in automated assays for bilirubin at multiple analyte concentrations.

The negative interference caused by haemolysis in manual bilirubin assays contrasts with the positive interference reported for some automated methods utilizing the same basic chemistry. A comparison was therefore made of the haemolysis interference associated with several automated bilirubin methodologies: multilayer film (Kodak Ektachem- total bilirubin (TBil), direct bilirubin (DBil), conjugated bilirubin (Bc), unconjugated bilirubin (Bu),; tableted reagents (Baxter Paramax- TBil, DBil); continuous flow (Technicon SMAC - TBil). Thirty serum pools were analysed (five concentrations of bilirubin, 2-229 mumol/l; six concentrations of haemoglobin, 0.