Temporal and Quantitative Transcriptomic Differences Define Sexual Dimorphism in Murine Postnatal Bone Aging.

Time is a central element of the sexual dimorphic patterns of development, pathology, and aging of the skeleton. Because the transcriptome is a representation of the phenome, we hypothesized that both sex and sex-specific temporal, transcriptomic differences in bone tissues over an 18-month period would be informative to the underlying molecular processes that lead to postnatal sexual dimorphism. Regardless of age, sex-associated changes of the whole bone transcriptomes were primarily associated not only with bone but also vascular and connective tissue ontologies.

On-Site, On-Demand 3D-Printed Nasopharyngeal Swabs to Improve the Access of Coronavirus Disease-19 Testing.

Diagnostic testing that facilitates containment, surveillance, and treatment of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), or future respiratory viruses, depends on a sample collection device that efficiently collects nasopharyngeal tissue and that can be manufactured on site when an outbreak or public health emergency is declared by a government. Here two novel stereolithography-based three-dimensional (3D)-printed nasopharyngeal swabs are reported which are made using a biocompatible and sterilizable photoresist. Such swabs are readily manufactured on-site and on-demand to ensure availability, if supply chain shortages emerge.

Structure-function relationships of the human vertebral endplate.

Background: Although deformation and fracture of the vertebral endplate have been implicated in spinal conditions such as vertebral fracture and disc degeneration, few biomechanical studies of this structure are available. The goal of this study was to quantify the mechanical behavior of the vertebral endplate.

Methods: Eight-five rectangular specimens were dissected from the superior and/or inferior central endplates of human lumbar spine segments L1 to L4.

Nonreciprocal Magnetic Coupling Using Nonlinear Meta-Atoms.

Breaking Lorentz reciprocity is fundamental to an array of functional radiofrequency (RF) and optical devices, such as isolators and circulators. The application of external excitation, such as magnetic fields and spatial-temporal modulation, has been employed to achieve nonreciprocal responses. Alternatively, nonlinear effects may also be employed to break reciprocity in a completely passive fashion.

Simultaneous Localization and Parameter Estimation for Single Particle Tracking via Sigma Points based EM.

Single Particle Tracking (SPT) is a powerful class of tools for analyzing the dynamics of individual biological macromolecules moving inside living cells. The acquired data is typically in the form of a sequence of camera images that are then post-processed to reveal details about the motion. In this work, we develop an algorithm for jointly estimating both particle trajectory and motion model parameters from the data.

Robust Visualization and Discrimination of Nanoparticles by Interferometric Imaging.

Single-molecule and single-nanoparticle biosensors are a growing frontier in diagnostics. Digital biosensors are those which enumerate all specifically immobilized biomolecules or biological nanoparticles, and thereby achieve limits of detection usually beyond the reach of ensemble measurements. Here we review modern optical techniques for single nanoparticle detection and describe the single-particle interferometric reflectance imaging sensor (SP-IRIS).

Dictums for problem solving and approximation in mathematical acoustics: examples involving low-frequency vibration and radiation.

A sequence of dictums for mathematical acoustics is given representing opinions intended to be regarded as authoritative, but not necessarily universally agreed upon. The dictums are presented in the context of the detailed solution for a class of problems involving the forced vibration of a long cylinder protruding half-way into a half-space bounded by a compliant surface (impedance boundary) characterized by a spring constant. One limiting case corresponds to a cylinder vibrating within an infinite rigid baffle, and another limiting case corresponds to a vibrating cylinder on the compliant surface of an incompressible fluid.