Efficacy of using trimethoprim-sulfamethoxazole prophylaxis in an adult patient with autoimmune neutropenia.

A 63-year-old man was admitted for pneumonia with neutropenia. After the pneumonia resolved with administration of antibiotics and granulocyte colony-stimulating factor, he was diagnosed with autoimmune neutropenia (AIN) on the basis of bone marrow findings and positive antineutrophil antibodies. He had recurring high fever once or twice a month with productive cough and an elevated serum C-reactive protein level.

A comparative proteomics study of a synovial cell line stimulated with TNF-α.

To elucidate the pathogenesis of rheumatoid arthritis (RA), we used proteomic analysis to determine the protein profile in a synovial cell line, MH7A, established from patients with RA. Proteins were extracted from MH7A cells that were or were not stimulated with tumor necrosis factor-α (TNF-α), and then analyzed on a liquid chromatography/mass spectrometry system equipped with a unique long monolithic silica capillary. On the basis of the results of this proteomic analysis, we identified 2650 proteins from untreated MH7A cells and 2688 proteins from MH7A cells stimulated with TNF-α.