Do different wheat ploidy levels respond differently against stripe rust infection: Interplay between reactive oxygen species (ROS) and the antioxidant defense system?

Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst) is the most damaging wheat disease, causing substantial losses in global wheat production and productivity.

Evaluating the specificity of flavivirus proteases in Aedes aegypti cells for dengue virus 2-derived cleavage sites.

Flaviviruses are a diverse group of RNA viruses known for their significant impact on human health worldwide. We generated a series of reporters that included cleavage sequences from the dengue virus type 2 polyprotein and co-transfected with plasmids encoding various flavivirus proteases into Aedes aegypti cells, followed by fluorescent imaging and western blot analysis for the determination of proteolytic cleavage. Recombinant flavivirus NS2B3 proteases from medically significant and insect-specific flaviviruses were able to process reporters encoding cleavage sequences from the dengue virus type 2 polyprotein in vitro including proteases from dengue virus types 1-4, Zika virus, yellow fever virus, Aedes flavivirus, and cell-fusing agent virus.

African bat database: curated data of occurrences, distributions and conservation metrics for sub-Saharan bats.

Accurate knowledge of species distributions is foundational for effective conservation efforts. Bats are a diverse group of mammals, with important roles in ecosystem functioning. However, our understanding of bats and their ecological importance is hindered by poorly defined ranges, mostly as a result of under-recording.

Desert diversification: revision of Bruner, 1908 (Orthoptera, Acrididae, Melanoplinae) with descriptions of sixteen new species from the United States and Mexico.

In this study, a morphological revision was conducted of Bruner, a group of grasshoppers inhabiting open xeric desert scrub, shrublands, and plains, spanning central Texas to central Mexico. The genus was originally described by Bruner in 1908, with two taxa added by Hebard in 1922. has remained unrevised despite numerous collections.

A chromosome-level reference genome for the common bed bug, Cimex lectularius, with identification of sex chromosomes.

The common bed bug, Cimex lectularius, is a globally distributed pest insect of medical, veterinary, and economic importance. Previous reference genome assemblies for this species were generated from short read sequencing data, resulting in a ~650 Mb composed of thousands of contigs. Here, we present a haplotype-resolved, chromosome-level reference genome, generated from an adult Harlen strain female specimen.

Stress drives premature hive exiting behavior that leads to death in young honey bee (Apis mellifera) workers.

Background: The Western honey bee, Apis mellifera, is an economically important pollinator, as well as a tractable species for studying the behavioral intricacies of eusociality. Honey bees are currently being challenged by multiple biotic and environmental stressors, many of which act concomitantly to affect colony health and productivity. For instance, developmental stress can lead workers to become precocious foragers and to leave the hive prematurely.

Fluralaner treatment of chickens kills the southern house mosquito, Culex quinquefasciatus.

The control of zoonotic and vector-borne pathogens is challenging due to the limited availability of intervention tools. West Nile virus (WNV) is an example of a globally distributed zoonotic arbovirus that circulates between Culex species (Diptera: Culicidae) mosquitoes and avian hosts, with spillover transmission to humans, resulting in disease cases. Interventions delivering systemic insecticides to vertebrate hosts used by vector species, known as xenointoxication, are potential tools for managing vector populations by creating toxic bloodmeals.

The natural history of Amblyomma maculatum sensu lato, a vector of Rickettsia parkeri rickettsiosis, in southern Arizona.

Amblyomma maculatum sensu lato (s.l.) is an ixodid tick found in the semi-arid southwestern United States and northern Mexico where it is a parasite of medical and veterinary significance, including as a vector for Rickettsia parkeri, a cause of spotted fever rickettsiosis in the Americas.

Epigenetic modulation of fungal pathogens: a focus on .

Epigenetics has emerged as a potent field of study for understanding the factors influencing the effectiveness of human disease treatments and for identifying alternations induced by pathogens in host plants. However, there has been a paucity of research on the epigenetic control of the proliferation and pathogenicity of fungal plant pathogens. Fungal plant pathogens such as , a significant threat to global rice production, provide an important model for exploring how epigenetic mechanisms govern fungal proliferation and virulence.

A footworm in the door: revising Onchocerca phylogeny with previously unknown cryptic species in wild North American ungulates.

Onchocerca is an important genus of vector-borne filarial nematodes that infect both humans and animals worldwide. Many Onchocerca spp., most of medical and veterinary health relevance, are the focus of a variety of diagnostic and molecular research.

Assessing fall armyworm () allochronic behavior as a predictor of local strain composition in United States populations.

The fall armyworm, (J.E. Smith), is comprised of two genetically distinct strains that are morphologically identical yet exhibit differences in their behavior and physiology (C-strain and R-strain).

Repeat mediated excision of gene drive elements for restoring wild-type populations.

Here, we demonstrate that single strand annealing (SSA) can be co-opted for the precise autocatalytic excision of a drive element. We have termed this technology Repeat Mediated Excision of a Drive Element (ReMEDE). By engineering direct repeats flanking the drive allele and inducing a double-strand DNA break (DSB) at a second endonuclease target site within the allele, we increased the utilization of SSA repair.

Evolutionary History and Ecology of () : A New Nearctic Mining Bee (Hymenoptera, Andrenidae) Species and Subgenus.

With about 1700 described species, the mining bee genus is a rapidly diversifying lineage and one of the most species-rich groups of bees. Recent phylogenomic advances have greatly improved our understanding of the phylogeny of the genus, yet many species still await description, subgeneric assignments that are in line with their evolutionary history, as well as study of their morphology and behavior. Here we provide a comprehensive account of a newly discovered species, n.

Bacterial Communities of House Flies from Beef and Dairy Cattle Operations are Diverse and Contain Pathogens of Medical and Veterinary Importance.

Adult house flies (Musca domestica L.) are important reservoirs and mechanical vectors of bacteria in livestock operations. House fly bacterial communities are influenced by their local environment, yet a comprehensive understanding of bacterial diversity, pathogen prevalence, and bacterial source is not fully understood.

The V410L kdr allele in the VGSC confers higher levels of field resistance to permethrin in urban mosquito populations of Aedes aegypti (L.).

Background: Females of Aedes aegypti transmit emerging arboviruses including Zika, dengue, yellow fever, and chikungunya. Control of these adult mosquitoes heavily relies on synthetic insecticides, including pyrethroids. However, insecticide resistance development in populations poses a significant challenge to vector control, particularly from knockdown resistance (kdr) mutations in the voltage-gated sodium channel (VGSC), the target of pyrethroids.

Comparison of Growth and Composition of Black Soldier Fly ( L.) Larvae Reared on Sugarcane By-Products and Other Substrates.

Black soldier fly larvae (BSFL) can convert organic waste into high-quality biomass. In this study, we tested the potential of sugarcane by-products as a food source for BSFL and compared larval development and nutritional value with some other organic substrates. Seven different substrates were used, including carrot pomace (C), carrot pomace and leftover bread (CB) (50/50), bagasse and vinasse (BV), bagasse and molasses (BM), bagasse, vinasse, and molasses (BVM), a mixture of all the above treatments (MX), and university canteen leftovers (UCLs).

Sex-Specific Perching: Monitoring of Artificial Plants Reveals Dynamic Female-Biased Perching Behavior in the Black Soldier Fly, (Diptera: Stratiomyidae).

Artificial perches are implemented by many companies that mass-rear the black soldier fly (BSF), to emulate a natural breeding environment or provide additional surface area for flies to rest; however, basic information about perching behavior is lacking. This experiment tested the effect of adding 0.00, 0.

Type VI secretion systems promote intraspecific competition and host interactions in a bee gut symbiont.

The Type VI Secretion System (T6SS) is a sophisticated mechanism utilized by gram-negative bacteria to deliver toxic effector proteins into target cells, influencing microbial community dynamics and host interactions. In this study, we investigated the role of T6SSs in wkB2, a core bacterial symbiont of the honey bee gut microbiota. We generated single- and double-knockout mutants targeting essential genes ( and ) in both T6SS-1 and T6SS-2 and assessed their colonization and competition capabilities in vivo.