Development and validation of a high throughput multiplex immunofluorescence assay to detect all human immunoglobulin isotypes and subclasses in human fluids.

Antibodies in human milk protect infants against infections, but currently no assay is described that is able to simultaneously measure all 9 antibody isotypes and subclasses immunoglobulins in human fluids, such as human milk. Our cohort "Protecting against Respiratory tract Infections through human Milk Analysis" (PRIMA) is focused on the relation between the occurrence of respiratory infections during the first year of life and concentration of maternal antibodies in breastfeeding. We developed and successfully validated a multiplex assay that is able to measure all nine antibody isotypes and subclasses in human plasma and milk (regardeless of the pathogen specificity), using a small sample volume.

Fungal Necrotizing Otitis Externa: Clinical and Therapeutic Features.

To describe the clinical and therapeutic features of fungal necrotizing otitis externa (NOE) as well as to identify the predictive factors of complications. This retrospective and monocentric study included 15 cases of fungal NOE treated in our ENT department between 2006 and 2024. Clinical, biological, microbiological, and imaging data were collected and evaluated.

Computerized Dynamic Posturography-Guided Vestibular Rehabilitation Improves Vestibular Sensory Ratios.

Vestibular deficits are common and debilitating, and many patients struggle with dynamic balance, even after treatment with standard rehabilitation techniques. The objective of this study was to measure changes in computerized dynamic posturography sensory ratio information after computerized vestibular retraining therapy (CVRT). This prospective, single-group, interventional study enrolled adult participants with stable, unilateral vestibular deficits.

Palatal Perforation Due to Nasal Myiasis in a Patient With Atrophic Rhinitis: A Case Report.

Atrophic rhinitis (AR) is a chronic nasal disease characterized by atrophy of the nasal mucosa and turbinates. Occasionally, nasal myiasis complicates AR. This case illustrates an uncommon complication of nasal myiasis, palatal perforation.

Comparison of Myringitis Incidence in Cartilage Myringoplasty With and Without External Ear Canal Packing For the Repair of Chronic Tympanic Membrane Perforations.

We compared the long-term efficacy and incidence of myringitis after cartilage graft underlay myringoplasty for chronic tympanic membrane (TM) perforations with and without external ear canal (EAC) packing. : In total, 129 patients who met the inclusion criteria were allocated to either the EAC packing (packing) group or the no-EAC packing (no-packing) group. All patients underwent endoscopic cartilage graft underlay myringoplasty.

MAP4K4 aggravates microvascular anomalies in diabetic retinopathy in a YTHDF2-dependent manner.

Aims/hypothesis: Signalling pathways that regulate endothelial cell (EC) dysfunction, ischaemia and inflammation play a crucial role in retinal microangiopathy such as diabetic retinopathy. MAP4K4 is highly expressed in ECs. However, the involvement of MAP4K4 in retinal vasculopathy of diabetic retinopathy remains unclear.

Device Survival After Pediatric Cochlear Implant Surgery: A 15-Year Single-Center Retrospective Analysis.

Objective: To evaluate device survival and identify risk factors for failure in pediatric cochlear implant (CI) surgery to guide strategies for minimizing failure rates and improving survival outcomes.

Methods: A retrospective analysis was conducted on pediatric patients who underwent CI surgery at the Children's Hospital, Zhejiang University School of Medicine, from September 2008 to September 2023. Device survival was assessed using the Kaplan-Meier method while independent factors influencing device survival were analyzed using the log-rank test and Cox regression model.

σ-σ conjugation Across Si─O─Si Bonds.

Polysiloxanes and silsesquioxanes (SQs) are known to be insulating materials. We describe here polysiloxane copolymers where this is not the case. Thus,MeVinylSi─O─SiMeVinyl/Br-Ar-Br copolymers exhibit conjugation via Si─O─Si bonds contrary to the widespread understanding that such linkages must be insulating.

Melioidosis of the Parotid Gland: Lessons Learned from a Fatal Case.

Meliodosis is an infectious disease also called whitemore's disease that can be fatal and is caused by the bacteria . This facultative intracellular aerobic gram-negative bacterium is typically transmitted through direct contact with contaminated water or soil (Currie et al. in Seminars in Respiratory and Critical Care Medicine 36:111-125, 2015).

A Systematic Review of Treatment Protocols in Auricular Pseudocysts.

A systematic review of literature on treatment modalities available with respect to efficacy, complications and recurrences so as to guide the clinician to better treat this enigmatic condition. A systematic search of PubMed, Google Scholar, Ovid and Cochrane databases. Articles in English that described or compared treatment were included.

Round Window Membrane Visibility and its Implications in Cochlear Implant Surgery.

With increasing number of patients with residual hearing being implanted, there is a renewed interest in round window (RW) as the preferred route for electrode insertion to reduce intracochlear trauma. The degree of round window membrane (RWM) visibility and its orientation might hamper the accessibility of RW for electrode insertion. This study is an attempt to identify the various factors affecting the accessibility of RW for electrode insertion.

A Rare Case of Non-Invasive Fungal Sinusitis Caused by Scedosporium Apiospermum in an Immunocompetent Patient.

Unlabelled: Fungal sinusitis are very often caused by Aspergillus spp. Dematiaceae spp. and mucomycoses.

Evaluation of Accuracy of Clinical and Preoperative HRCT Diagnostic Findings with Intraoperative Findings in Patients of Cholesteatoma.

Cholesteatoma is a progressive and destructive middle ear disease that can lead to severe complications such as hearing loss, bone erosion, and even intracranial infections if left untreated. Accurate preoperative evaluation is crucial for the successful surgical management of this condition. High-Resolution Computed Tomography (HRCT) has become the gold standard in evaluation of extent of cholesteatoma due to its ability to reveal anatomical details of the middle ear.

Cavernous Hemangioma of the Palatine Tonsil: A Case Report and Review of the Literature.

Unlabelled: Cavernous hemangiomas are venous malformations characterized by interconnected channels of abnormally dilated blood vessels having low flow of blood. Whilst venous malformations most commonly affect the head and neck region, isolated cavernous hemangiomas of the palatine tonsils are extremely rare; with only 4 cases being reported in the English Literature. We report a case of a 43-year-old female who was referred to us for the evaluation and management of a right tonsillar swelling.

Videonystamography Findings in Dizziness Patients with Background Anxiety Disorder.

This study was conducted to describe the videonystagmography findings in dizziness patients with background anxiety disorders. It was a hospital-based, descriptive analytical study, conducted among 64 adult patients with diagnosed anxiety disorders presenting with complaint of dizziness attending the vertigo clinic run by the Department of ENT at St. John's Medical College and Hospital, Bangalore, over a period of 24 months.

Paediatric Head and Neck Hemangiomas: Scopes, Challenges and Outcome of Surgical Management.

Haemangiomas are benign vascular tumours frequently encountered in head and neck region. The different systems of nomenclature leading to terminological confusion and management protocols for vascular lesions have been contentious issues and have undergone substantial paradigm shift over the years. A policy has been the mainstay of treatment owing to spontaneous regression of majority of paediatric hemangiomas along with sclerotherapy, laser therapy, drug therapy and radiotherapy.

Elderly Female with Cranial Polyneuropathy.

Unlabelled: The recrudescence of Varicella Zoster Virus in the head and neck region often manifests as Ramsay Hunt Syndrome, characterised by facial nerve palsy, vesicular rash in the distribution of facial nerve and neuralgia. Rarely it causes cranial polyneuropathy (CP). We present a case of herpes zoster with CP, highlighting the diagnostic challenges and management in a resource-limited setting.

A comparative study of pre- and post-rhinoplasty patients' quality of life (QOL) in the lower socio-economic demography.

Objective: Rhinoplasty also popularly known among people as plastic surgery performed on nose.it is the most commonly performed plastic surgery procedures for both functional and aesthetic purposes according to the statistics of the American Society of Plastic Surgeons [1]. This study is aimed to investigate the changes in the psychological impact of rhinoplasty on day-to-day life & on quality of life after the rhinoplasty surgery.

Odontogenic Sinusitis: Endoscopic Management of this Iatrogenic Entity.

Odontogenic maxillary sinusitis (OMS) is a condition presenting to both the dental and otolaryngologic practitioner. Common causes of OMS include dental implants, displacement of a maxillary tooth root tip during extraction, migration of materials used in root canal therapy or graft material in sinus lift procedure. A 68-year-old male patient presented with complaints of repeated episodes of sinusitis for about 3 months which was not responding to conservative management.

A Correlational Study of Tinnitus Handicap Inventory (THI) Scores and Otoacoustic Emissions (OAE) Among Tinnitus Patients.

To determine the level of disability in patients suffering from tinnitus using the Tinnitus Handicap Inventory (THI) score, to evaluate the changes in otoacoustic emissions in a patient with tinnitus and evaluate the co-relation of the Tinnitus Handicap Inventory score with the Otoacoustic emission result This is an observational study done over a period of 6 months, in which patients aged more than 18 years who presented to the ENT OPD of our institute with complaints of tinnitus were considered for inclusion in the study. Patients with primary tinnitus, who consented to be part of the study were assessed for handicap caused by tinnitus using the Tinnitus Handicap Inventory (THI) score. All patients were subjected to Pure Tone Audiometry (PTA) and Screening OAE to look for the "Pass" or "Refer" in the OAE result.

Effect of Smoking on Electron Microscopic Structure of Nasal Mucosa.

The present study aimed to assess and compare the effect of smoking on ultrastructure morphology of nasal mucosa between smokers and nonsmokers. The study included 50 subjects aged 25-62 years who were planned for rhinological surgical procedures. Subjects were divided into 3 groups- group 1 (nonsmoker), group 2 (smokers taking 1 pack/day or less), group 3 (smokers taking 2 pack/day or more).