8 results match your criteria: "Department of Dairy Science Virginia Polytechnic Institute and State University Blacksburg[Affiliation]"
Follicular development and ovulation were examined in superovulated Nubian and Nubian-cross dairy goats following prostaglandin F(2alpha) (PGF(2alpha)) and/or gonadotropin releasing hormone (GnRH) treatment. Estrus was synchronized with Synchromate-B((R)) implants. Superovulation was induced with follicle stimulating hormone (FSH) and augmented with GnRH and/or PGF(2alpha).
View Article and Find Full Text PDFIn Experiment 1, development of zona pellucida-intact (ZPI) morulae was measured every 24 hours for 120 hours after encapsulation in 2% sodium alginate (ALG) or 0.1% poly-L-lysine (PLL). Encapsulation significantly reduced development to hatched blastocysts at 48 and 72 hours.
View Article and Find Full Text PDFCrossbred gilts and sows (n=116) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality for zygote collection. Four synchronization and superovulation procedures were used: 1) sows were observed for natural estrous behavior; 1000 IU human chorionic gonadotrophin (hCG) was administered at the onset of estrus (NAT); 2) cyclic gilts were synchronized with 17.
View Article and Find Full Text PDFOne-cell bovine embryos fertilized in vivo were cultured in TCM-199 and bovine oviductal epithelial cells, in TCM-199, or in explanted immature mouse oviducts supported by TCM-199 to compare development to the blastocyst stage. The morphological stage of development and cell number were determined following 144 hours of culture. Of the embryos that cleaved at least once, 52.
View Article and Find Full Text PDFThis study was conducted to examine the effect of endothelial cell growth supplement (ECGS) and fibroblastic growth factor (FGF) on early mammalian embryo development in vitro. Two hundred mouse blastocysts were placed randomly in culture wells containing one of five treatments: 1) Ham's F-10, 2) Ham's F-10 + 10 mug ECGS, 3) Ham's F-10 + 10 ng ECGS, 4) Ham's F-10 + 100 ng FGF and 5) Ham's F-10 + 10 ng FGF. In all cases, media were supplemented with 10% (v/v) normal steer serum.
View Article and Find Full Text PDFOne hundred fifty-three excellent and good bovine morulae were cultured in Ham's F-10, supplemented with 10 % steer serum, bovine serum albumin, or uterine flushings (64 mg protein/ml) to compare embryo development. Embryos were observed every 12 h in culture. Treatment differences were evaluated by assigning a numerical value of 0 to 4 to each embryo representing the stage of development reached in vitro.
View Article and Find Full Text PDFThis study was conducted to compare in vitro development of bovine morulae in Ham's F-10 and Dulbecco's phosphate buffered saline (D-PBS) media supplemented with 10% (v/v) normal steer serum. Fifty-three excellent and good embryos were obtained by superovulating 15 non-lactating Holstein cows. Embryos were placed randomly in culture with Ham's F-10 or D-PBS media and development was recorded at 12-h intervals for the duration of culture.
View Article and Find Full Text PDFThis study evaluated the feasibility of using the Fossomatic somatic cell counter in the Dairy Herd Improvement Association (DHIA). DHIA utilizes preserved milk samples which have usually been stored for 1-5 days. Milk samples were stored for various periods, up to 7 days, and were preserved with (a) dichromate at 5 C, (b) dichromate at ambient temperatures, or (c) without dichromate at 5 C.
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