280 results match your criteria: "Department of Clinical Molecular Medicine[Affiliation]"

Epidemiological characteristics of mycoplasma pneumoniae in hospitalized children before, during, and after COVID-19 pandemic restrictions in Chongqing, China.

Front Cell Infect Microbiol

September 2024

Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Child Rare Diseases in Infection and Immunity, Chongqing, China.

Article Synopsis
  • - Mycoplasma pneumoniae (MP) is a major cause of pneumonia, particularly in children, with high rates of resistance to macrolide antibiotics; the study analyzed trends in MP infections across different phases of the COVID-19 pandemic.
  • - Out of 36,685 children with pneumonia treated between 2019 and 2023, 20.74% tested positive for MP, with the highest infection rates in children over 6 years old and during summer, while the lowest rates were observed during the pandemic itself.
  • - Post-pandemic, there was a notable increase in MP infections among younger children (ages 3.8 to 13.5), along with a high macrolide resistance rate (79
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Background:  Increasing evidence suggests that diabetes increases the risk of developing different types of cancer. Hyperinsulinemia, hyperglycemia and chronic inflammation, characteristic of diabetes, could represent possible mechanisms involved in cancer development in diabetic patients. At the same time, cancer increases the risk of developing new-onset diabetes, mainly caused by the use of specific anticancer therapies.

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Background: Earlier studies have estimated the impact of increased body mass index (BMI) on healthcare costs. Various methods have been used to avoid potential biases and inconsistencies. Each of these methods measure different local effects and have different strengths and weaknesses.

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Article Synopsis
  • Immunotherapy using immune checkpoint inhibitors (ICI) is becoming common in cancer treatment, but managing related endocrine side effects can be challenging.
  • Different scientific societies have varying guidelines concerning when to conduct endocrine testing for patients undergoing this therapy.
  • A panel of experts from several Italian medical associations has developed a straightforward and practical checklist for assessing endocrine and metabolic health in cancer patients receiving immunotherapy.
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Lynch Syndrome (LS) is a hereditary cancer syndrome caused by pathogenic germline variants in one of the four mismatch repair (MMR) genes MLH1, MSH2, MSH6 and PMS2. It is characterized by a significantly increased risk of multiple cancer types, particularly colorectal and endometrial cancer, with autosomal dominant inheritance. Access to precise and sensitive methods for genetic testing is important, as early detection and prevention of cancer is possible when the variant is known.

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There is increasing evidence of the role of endocrine disruptors (EDs) derived from commonly employed compounds for manufacturing and processing in altering hormonal signaling and function. Due to their prolonged half-life and persistence, EDs can usually be found not only in industrial products but also in households and in the environment, creating the premises for long-lasting exposure. Polybrominated diphenyl ethers (PBDEs) are common EDs used in industrial products such as flame retardants, and recent studies are increasingly showing that they may interfere with both metabolic and oncogenic pathways.

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[Potential Oncogenic Role of Genes in PLC Family on T-ALL Cell Lines by Suppression of Apoptosis].

Zhongguo Shi Yan Xue Ye Xue Za Zhi

August 2023

Department of Clinical Molecular Medicine of Children's Hospital in Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China,Clinical Research Unit of Children's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine; Institute of Pediatric Infection, Immunity and Critical Care Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200062, China,E-mail:

Article Synopsis
  • The study investigates how the phospholipase C (PLC) family contributes to the development and progression of acute T lymphoblastic leukemia (T-ALL).
  • Methods included analyzing cell death in T-ALL cells after using PLC inhibitors and assessing the influence of PLC expressions on patient survival using various statistical analyses.
  • Results indicated that certain T-ALL cell lines were sensitive to PLC inhibitors, while others were resistant, and high expression of specific PLC genes was linked to poor patient prognosis, suggesting these genes could be potential targets for therapy.
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[Neonatal growth retardation and lactic acidosis initiated by novel mutation sites in gene].

Zhonghua Yu Fang Yi Xue Za Zhi

June 2023

Department of Clinical Molecular Medicine of Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China Clinical Research Unit of Children's Hospital in Shanghai Jiao Tong University School of Medicine, Institute of Pediatric Infection, Immunity, and Critical Care Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200062, China.

This study aims to analyze the clinical characteristics and genetic variations of two cases with developmental delay and lactic acidosis in a family, and to explore the relationship between genetic variations and clinical features. A retrospective analysis was conducted on the clinical characteristics of two siblings with developmental delay and lactic acidosis who were treated at the Neonatal Department of Children's Hospital of Chongqing Medical University in May 2019 and December 2021, respectively. Whole-exome sequencing was used to detect genetic variations in the affected children.

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Sex-Specific Survival Bias and Interaction Modeling in Coronary Artery Disease Risk Prediction.

Circ Genom Precis Med

February 2023

Division of Cardiovascular Medicine, Department of Internal Medicine (I.S., W.E.H., N.R.S., C.J.W.), University of Michigan, Ann Arbor.

Background: The 10-year Atherosclerotic Cardiovascular Disease risk score is the standard approach to predict risk of incident cardiovascular events, and recently, addition of coronary artery disease (CAD) polygenic scores has been evaluated. Although age and sex strongly predict the risk of CAD, their interaction with genetic risk prediction has not been systematically examined. This study performed an extensive evaluation of age and sex effects in genetic CAD risk prediction.

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[The Effect of KRAS on Proliferation and Apoptosis of T-ALL Cell Lines].

Zhongguo Shi Yan Xue Ye Xue Za Zhi

August 2022

Department of Clinical Molecular Medicine of Children's Hospital in Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China,Clinical Research Unit of Children's Hospital in Shanghai Jiaotong University; Institute of Pediatric Infection, Immunity and Critical Care Medicine, Shanghai Jiaotong University School of Medicine, Shanghai 200062, China,E-mail:

Objective: To investigate the function of RAS protein on the progression of the T-ALL cell lines in vitro.

Methods: The DNA of the T-ALL cells was purified then amplified the coding regions of three RAS genes (KRAS, NRAS, HRAS) by PCR reaction. After T-A cloning, the coding regions of KRAS, NRAS and HRAS were sequenced by Sanger Sequencing.

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[Application of Flow Cytometry Combined Fluorescence in Situ Hybridization to Indentify the Lymphocyte Subtypies with Epstein-Barr Virus Infection].

Zhongguo Shi Yan Xue Ye Xue Za Zhi

June 2022

Department of Clinical Molecular Medicine of Children's Hospital in Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China;Clinical Research Unit of Children's Hospital in Shanghai Jiaotong University; Institute of Pediatric Infection, Immunity, and Critical Care Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200062, China,E-mail:

Article Synopsis
  • The study aimed to develop a Flow-FISH technique to detect Epstein-Barr virus (EBV) infected lymphocytes in patient blood samples.
  • The methods included isolating blood monocytes, detecting specific RNA and cell surface markers, and optimizing conditions for cell integrity and hybridization.
  • The successful establishment of Flow-FISH allows for the identification of EBV cell subtypes, paving the way for its use in clinical testing for EBV-related diseases.
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Hypoxic preconditioning improves the survival and pro-angiogenic capacity of transplanted human umbilical cord mesenchymal stem cells via HIF-1α signaling in a rat model of bronchopulmonary dysplasia.

Biochem Biophys Res Commun

May 2022

Pediatric Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Engineering Research Center of Stem Cell Therapy, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China; Department of Clinical Molecular Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China; Clinical Research Unit, Children's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200062, China; Institute of Pediatric Infection, Immunity, and Critical Care Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai, 200062, China. Electronic address:

Bronchopulmonary dysplasia (BPD) is a serious chronic respiratory disease that predominates in the neonatal period. Currently, efficacious and effective specific treatments are lacking. Mesenchymal stem cells (MSCs) transplantation has emerged as a promising option for treating BPD.

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Gastroenteropancreatic neuroendocrine tumors (GEP-NETs) are a heterogeneous group of malignancies derived from neuroendocrine cells that can occur anywhere along the gastrointestinal tract. GEP-NETs incidence has been steadily increasing over the past decades, in parallel with the increasing incidence of the metabolic syndrome (MetS). It is not yet fully known whether the MetS components (such as obesity, dyslipidemia and type 2 diabetes) could be involved in the etiology of GEP-NETs or could influence their outcomes.

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Pancreatic cancer (PC) is a common cause of cancer-related death, due to difficulties in detecting early-stage disease, to its aggressive behaviour, and to poor response to systemic therapy. Therefore, developing strategies for early diagnosis of resectable PC is critical for improving survival. Diabetes mellitus is another major public health problem worldwide.

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In this review, two types of soft-tissue involvement in multiple myeloma are defined: (i) extramedullary (EMD) with haematogenous spread involving only soft tissues and (ii) paraskeletal (PS) with tumour masses arising from skeletal lesions. The incidence of EMD and PS plasmacytomas at diagnosis ranges from 1·7% to 4·5% and 7% to 34·4% respectively. EMD disease is often associated with high-risk cytogenetics, resistance to therapy and worse prognosis than in PS involvement.

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Improved transfemoral accessibility and positioning of the Portico transcatheter heart valve with the new FlexNav delivery system.

Future Cardiol

July 2021

Clinical & Interventional Cardiology Department, IRCCS Policlinico San Donato, San Donato Milanese, Milan, Italy.

The new iteration of the Portico transcatheter heart valve delivery system, the FlexNav, has been designed to enhance procedural safety and accuracy of the platform. Major technological advancements were the significant reduction of the insertion profile, allowing a transfemoral access down to 5.0 mm vessels, and the addition of a stability layer, improving deployment accuracy and thus potentially reducing implantation depth and permanent pacemaker implantation rate.

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Background: Limited knowledge exists on the expected long-term effects and cost-effectiveness of initiatives aiming to reduce the burden of obesity.

Aim: To develop a Norwegian obesity-focused disease-simulation model: the MOON model.

Material And Methods: We developed a Markov model and simulated a Norwegian birth cohort's movement between the health states "normal weight,""overweight,""obese 1,""obese 2," and "dead" using a lifetime perspective.

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Bronchopulmonary dysplasia (BPD) is a serious chronic lung disease in premature newborns, with high morbidity and mortality rates. Mesenchymal stem cell (MSC) transplantation has developed into a promising approach to alleviate BPD. Small extracellular vesicles, which are an important therapeutic component of MSCs, have been reported to be effective in a mouse model of BPD.

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Background: Telomeres are crucial parts of chromosomes that protect the genome. They shorten every time the cell replicates, and shorter telomeres have been associated with increasing age and with many health behaviours. There is inconclusive evidence on the association between physical activity (PA) and telomere length.

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Adding pertuzumab to standard trastuzumab-based adjuvant therapy significantly improved invasive disease-free survival (IDFS) in the APHINITY trial. However, the magnitude of benefit was marginal in the overall population. We used GRADE (Grading of Recommendations Assessment, Development and Evaluation) analysis on data from APHINITY to build summary-of-findings tables to evaluate the efficacy, safety and quality of evidence of predefined clinical outcomes for the addition of pertuzumab to trastuzumab-based adjuvant therapy in patients with high-risk HER2-positive early breast cancer.

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The future of pharmacogenetics in the treatment of migraine.

Pharmacogenomics

November 2019

Department of Neurosciences, Mental Health & Sensory Organs (NESMOS), Sapienza University of Rome, Rome, Italy.

Migraine is considered one of the most disabling neurological disorder with a high socioeconomic burden. Pharmacological management includes many classes of drugs which in the most cases, are administrated in polytherapy. The therapeutic scheme of migraineurs is often affected by comorbidities which need concomitant medications, thus increasing the risk of side effects related to drug-drug interactions.

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Glucose-6-phosphate dehydrogenase (G6PD) deficiency is one of the most common X-linked enzymopathies caused by G6PD gene variant. We aimed to provide the characteristics of G6PD deficiency and G6PD gene variant distribution in a large Chinese newborn screening population. We investigated the prevalence of G6PD in China from 2013 to 2017.

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