Simultaneous Pharmacologic Inhibition of Yes-Associated Protein 1 and Glutaminase 1 via Inhaled Poly(Lactic-co-Glycolic) Acid-Encapsulated Microparticles Improves Pulmonary Hypertension.

Background Pulmonary hypertension (PH) is a deadly disease characterized by vascular stiffness and altered cellular metabolism. Current treatments focus on vasodilation and not other root causes of pathogenesis. Previously, it was demonstrated that glutamine metabolism, as catalyzed by GLS1 (glutaminase 1) activity, is mechanoactivated by matrix stiffening and the transcriptional coactivators YAP1 (yes-associated protein 1) and transcriptional coactivator with PDZ-binding motif (TAZ), resulting in pulmonary vascular proliferation and PH.

Application of Self-Interaction Corrected Density Functional Theory to Early, Middle, and Late Transition States.

Density functional theory (DFT)-based methods often significantly underpredict chemical reaction barriers compared with experiments because of the tendency of DFT to overstabilize transition states with stretched bonds due to the impact of unphysical electron self-interaction. However, many reactions have early or late transition states where the transition state geometry closely resembles the reactants or products, respectively. The role of self-interaction in those cases is not known.

Eliminating segregation in free-surface flows of particles.

By introducing periodic flow inversions, we show both experimentally and computationally that forcing with a value above a critical frequency can effectively eliminate both density and size segregation. The critical frequency is related to the inverse of the characteristic time of segregation and is shown to scale with the shear rate of the particle flow. This observation could lead to new designs for a vast array of particle processing applications and suggests a new way for researchers to think about segregation problems.