[Effect of glutamine on the expression of grp75 in PC12 cells].

Glutamine (Gln), a conditionally essential amino acid, can be a potential enhancer of the heat stress response. And glucose-regulated protein 75(grp75) is a member of the hsp family. To evaluate the effect of glutamine on the expression of grp75, PC12 cell was cultured with DMEM, glucose-free DMEM, DMEM within Gln, and glucose-free DMEM within Gln, and the expression of grp75 was detected by immunocytochemistry and western blot, the mRNA level of grp75 in the cells was detected by reverse transcriptase-polymerase chain reaction (RT-PCR).