190 results match your criteria: "Department of Biology and Biotechnology "C. Darwin" Sapienza University of Rome[Affiliation]"
Int J Mol Sci
September 2021
Department of Pharmacy, University of Naples Federico II, Via D. Montesano 49, 80131 Naples, Italy.
DNA G-quadruplex (G4) structures, either within gene promoter sequences or at telomeres, have been extensively investigated as potential small-molecule therapeutic targets. However, although G4s forming at the telomeric DNA have been extensively investigated as anticancer targets, few studies focus on the telomeric repeat-containing RNA (TERRA), transcribed from telomeres, as potential pharmacological targets. Here, a virtual screening approach to identify a library of drug-like putative TERRA G4 binders, in tandem with circular dichroism melting assay to study their TERRA G4-stabilizing properties, led to the identification of a new hit compound.
View Article and Find Full Text PDFFEMS Microbiol Lett
October 2021
Department of Biology and Biotechnology "C. Darwin", Pasteur Institute-Cenci Bolognetti Foundation, Sapienza University of Rome, Piazzale A. Moro 5, 00185 Rome, Italy.
Neuropathol Appl Neurobiol
February 2022
Synaptic Immunopathology Lab, IRCCS San Raffaele Roma, Italy.
Plants (Basel)
August 2021
Department of Agricultural Sciences, University of Naples Federico II, Via Università 100, 80055 Portici, Italy.
The inhibitory effect of extracellular DNA (exDNA) on the growth of conspecific individuals was demonstrated in different kingdoms. In plants, the inhibition has been observed on root growth and seed germination, demonstrating its role in plant-soil negative feedback. Several hypotheses have been proposed to explain the early response to exDNA and the inhibitory effect of conspecific exDNA.
View Article and Find Full Text PDFNanomaterials (Basel)
August 2021
Institute of Higher Secondary Education "Presta-Columella", Technical and Technological Sector "Agriculture, Agri-Food and Agro-Industry", Via S. Pietro in Lama snc, 73100 Lecce, Italy.
Technological innovation, environmental sustainability, health, and wellness are the trajectories explored by current research to identify new strategies for a general improvement of human quality of life [...
View Article and Find Full Text PDFAntioxidants (Basel)
August 2021
Department of Physiology and Pharmacology "V. Erspamer", Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, Italy.
Long-term high-fat diet (HFD) consumption can cause weight gain and obesity, two conditions often associated with hepatic non-alcoholic fatty liver and oxidative stress. Oleoylethanolamide (OEA), a lipid compound produced by the intestine from oleic acid, has been associated with different beneficial effects in diet-induced obesity and hepatic steatosis. However, the role of OEA on hepatic oxidative stress has not been fully elucidated.
View Article and Find Full Text PDFThe impact of protein-coding genes on cancer onset and progression is a well-established paradigm in molecular oncology. Nevertheless, unveiling the contribution of the noncoding genes-including long noncoding RNAs (lncRNAs)-to tumorigenesis represents a great challenge for personalized medicine, since they (i) constitute the majority of the human genome, (ii) are essential and flexible regulators of gene expression and (iii) present all types of genomic alterations described for protein-coding genes. LncRNAs have been increasingly associated with cancer, their highly tissue- and cancer type-specific expression making them attractive candidates as both biomarkers and therapeutic targets.
View Article and Find Full Text PDFFEMS Microbiol Lett
August 2021
Department of Biology and Biotechnology "C. Darwin", Pasteur Institute-Cenci Bolognetti Foundation, Sapienza University of Rome, Piazzale A. Moro 5, 00185 Rome, Italy.
Transient modification of the environment involves the expression of specific genes and degradation of mRNAs and proteins. How these events are linked is poorly understood. CCR4-NOT is an evolutionary conserved complex involved in transcription initiation and mRNA degradation.
View Article and Find Full Text PDFFront Microbiol
July 2021
Department of Biology and Biotechnology 'C. Darwin', Sapienza University of Rome, Rome, Italy.
In unicellular organisms like yeasts, which do not have specialized tissues for protection against environmental challenges, the presence of cellular mechanisms to respond and adapt to stress conditions is fundamental. In this work, we aimed to investigate the response to environmental light in . Yeast lacks specialized light-sensing proteins; however, has been reported to respond to light by increasing hydrogen peroxide level and triggering nuclear translocation of Msn2.
View Article and Find Full Text PDFComput Struct Biotechnol J
May 2021
Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology, Dr. Aiguader 88, 08003 Barcelona, Spain.
Human serine hydroxymethyltransferase (SHMT) regulates the serine-glycine one carbon metabolism and plays a role in cancer metabolic reprogramming. Two SHMT isozymes are acting in the cell: SHMT1 encoding the cytoplasmic isozyme, and SHMT2 encoding the mitochondrial one. Here we present a molecular model built on experimental data reporting the interaction between SHMT1 protein and SHMT2 mRNA, recently discovered in lung cancer cells.
View Article and Find Full Text PDFChromosoma
September 2021
Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, Rome, Italy.
Heterochromatin protein 1 (HP1) is a non-histone chromosomal protein first identified in Drosophila as a major component of constitutive heterochromatin, required for stable epigenetic gene silencing in many species including humans. Over the years, several studies have highlighted additional roles of HP1 in different cellular processes including telomere maintenance, DNA replication and repair, chromosome segregation and, surprisingly, positive regulation of gene expression. In this review, we briefly summarize past research and recent results supporting the unexpected and emerging role of HP1 in activating gene expression.
View Article and Find Full Text PDFSci Total Environ
August 2021
Department of Chemistry, Sapienza University of Rome, 00185 Rome, Italy.
An intensive study, applied to a site characterized by multiple sources of microorganisms, was aimed at understanding the best approach to study bioaerosol. Culture-based, molecular biological, and chemical methods were applied to Particulate Matter (PM) samples collected in a livestock facility, during spring and autumn seasons, in two different outdoor areas. The first one was close to a place where feed was stored and handled and the second next to an open cowshed.
View Article and Find Full Text PDFSci Adv
June 2021
Institute of Molecular Biology and Pathology (IBPM), National Research Council (CNR) of Italy c/o Department of Biology and Biotechnology "C. Darwin, " Sapienza University, 00185 Rome, Italy.
H3K9 methylation maintains cell identity orchestrating stable silencing and anchoring of alternate fate genes within the heterochromatic compartment underneath the nuclear lamina (NL). However, how cell type-specific genomic regions are specifically targeted to the NL is still elusive. Using fibro-adipogenic progenitors (FAPs) as a model, we identified Prdm16 as a nuclear envelope protein that anchors H3K9-methylated chromatin in a cell-specific manner.
View Article and Find Full Text PDFPlant Cell Environ
September 2021
Department of Health, Life and Environmental Sciences, University of L'Aquila, L'Aquila, Italy.
Early signalling events in response to elicitation include reversible protein phosphorylation and re-localization of plasma membrane (PM) proteins. Oligogalacturonides (OGs) are a class of damage-associated molecular patterns (DAMPs) that act as endogenous signals to activate the plant immune response. Previous data on early phosphoproteome changes in Arabidopsis thaliana upon OG perception uncovered the immune-related phospho-regulation of several membrane proteins, among which PCaP1, a PM-anchored protein with actin filament-severing activity, was chosen for its potential involvement in OG- and flagellin-triggered responses.
View Article and Find Full Text PDFNat Rev Genet
September 2021
Department of Biology and Biotechnology "C. Darwin" and IIT CLN2S@Sapienza, Sapienza University of Rome, Rome, Italy.
Front Cell Dev Biol
April 2021
Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay.
Int J Environ Res Public Health
March 2021
Department of Biological and Environmental Sciences and Technologies, University of Salento, Via Prov.le Lecce-Monteroni, 73100 Lecce, Italy.
In recent years, lipid metabolism has gained greater attention in several diseases including cancer. Dysregulation of fatty acid metabolism is a key component in breast cancer malignant transformation. In particular, de novo lipogenesis provides the substrate required by the proliferating tumor cells to maintain their membrane composition and energetic functions during enhanced growth.
View Article and Find Full Text PDFSci Transl Med
March 2021
Department of Developmental Neurobiology, Graduate School of Life Sciences, Kumamoto University, 1-1-1 Honjo, Chuo-ku, Kumamoto 860-8556, Japan.
The lateral ventricle (LV) is flanked by the subventricular zone (SVZ), a neural stem cell (NSC) niche rich in extrinsic growth factors regulating NSC maintenance, proliferation, and neuronal differentiation. Dysregulation of the SVZ niche causes LV expansion, a condition known as hydrocephalus; however, the underlying pathological mechanisms are unclear. We show that deficiency of the proteoglycan Tsukushi (TSK) in ependymal cells at the LV surface and in the cerebrospinal fluid results in hydrocephalus with neurodevelopmental disorder-like symptoms in mice.
View Article and Find Full Text PDFAutophagy
January 2021
Hong Kong Baptist University, School of Chinese Medicine, Hong Kong, China.
Sci Data
December 2020
Department of Ecological and Biological Sciences (DEB), University of Tuscia, Largo Università snc, 01100, Viterbo, Italy.
Stressful experiences are part of everyday life and animals have evolved physiological and behavioral responses aimed at coping with stress and maintaining homeostasis. However, repeated or intense stress can induce maladaptive reactions leading to behavioral disorders. Adaptations in the brain, mediated by changes in gene expression, have a crucial role in the stress response.
View Article and Find Full Text PDFCurr Biol
February 2021
Institute of Molecular Biology and Pathology, National Research Council of Italy, c/o Department of Biology and Biotechnology, Sapienza University of Rome, Via degli Apuli 4, 00185 Rome, Italy. Electronic address:
Mitotic spindle orientation is a crucial process that defines the axis of cell division, contributing to daughter cell positioning and fate, and hence to tissue morphogenesis and homeostasis. The trimeric NuMA/LGN/Gαi complex, the major determinant of spindle orientation, exerts pulling forces on the spindle poles by anchoring astral microtubules (MTs) and dynein motors to the cell cortex. Mitotic kinases contribute to correct spindle orientation by regulating nuclear mitotic apparatus protein (NuMA) localization, among which the Aurora-A centrosomal kinase regulates NuMA targeting to the cell cortex in metaphase.
View Article and Find Full Text PDFInt J Mol Sci
November 2020
Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, 00185 Rome, Italy.
Extracellular vesicles (EVs) are widely investigated in glioblastoma multiforme (GBM) for their involvement in regulating GBM pathobiology as well as for their use as potential biomarkers. EVs, through cell-to-cell communication, can deliver proteins, nucleic acids, and lipids that are able to reprogram tumor-associated macrophages (TAMs). This research is aimed to concentrate, characterize, and identify molecular markers of EVs subtypes released by temozolomide (TMZ)-treated and non TMZ-treated four diverse GBM cells.
View Article and Find Full Text PDFCurr Opin Plant Biol
October 2020
Laboratory of Functional Genomics and Proteomics of Model Systems, Department of Biology and Biotechnology "C. Darwin", "La Sapienza" University of Rome, Via dei Sardi 70, Rome, Italy. Electronic address:
In multicellular organisms, growth is defined by those processes that allow an organ to increase in mass, namely cell proliferation - that increases the number of cells - and cell expansion - that increases their volume. For an organ to achieve a functional shape and a characteristic final size both these processes need to be tightly coordinated. In roots, these processes stand behind root primary growth, which results in lengthening of the root along its longitudinal axis, and secondary growth, which results in an increase of the root thickness.
View Article and Find Full Text PDFACS Cent Sci
September 2020
Department of Chemical Sciences, University of Naples Federico II, 80126 Naples, Italy.
The gut microbiota guide the development of the host immune system by setting a systemic threshold for immune activation. Lipopolysaccharides (LPSs) from gut bacteria are able to trigger systemic and local proinflammatory and immunomodulatory responses, and this capability strongly relies on their fine structures. Up to now, only a few LPS structures from gut commensals have been elucidated; therefore, the molecular motifs that may be important for LPS-mammalian cell interactions at the gut level are still obscure.
View Article and Find Full Text PDFInt J Mol Sci
September 2020
Blond McIndoe Laboratories, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester M13 9PT, UK.
Functional characterization of muscarinic cholinergic receptors in myelinating glial cells has been well described both in central and peripheral nervous system. Rat Schwann cells (SCs) express different muscarinic receptor subtypes with the prevalence of the M2 subtype. The selective stimulation of this receptor subtype inhibits SC proliferation, improving their differentiation towards myelinating phenotype.
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