The study investigates the effectiveness of DNA barcoding for identifying crayfish species, revealing that while local barcoding gaps exist, only a few genera show global barcoding gaps meeting the typical threshold for species discovery.
Analysis of mitochondrial COI sequence data from 81 crayfish species indicates that global barcoding gaps are below the previously suggested 10× threshold, leading researchers to propose a new ~5× threshold for better species identification.
The findings suggest that the existing taxonomy of most crayfish species may be insufficient, indicating a need for taxonomic revisions even for species with identifiable local barcoding gaps.