Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response.

Waterlogging leads to major crop losses globally, particularly for waterlogging-sensitive crops such as barley. Waterlogging reduces oxygen availability and results in additional stresses, leading to the activation of hypoxia and stress response pathways that promote plant survival. Although certain barley varieties have been shown to be more tolerant to waterlogging than others and some tolerance-related quantitative trait loci have been identified, the molecular mechanisms underlying this trait are mostly unknown.

A simple and efficient -mediated transient expression system to dissect molecular processes in and .

Unlabelled: The family Brassicaceae is a source of important crop species, including (oilseed rape), , and , that is used globally for oil production or as a food source ( pak choi or turnip). However, despite advances in recent years, including genome sequencing, a lack of established tools tailored to the study of crop species has impeded efforts to understand their molecular processes in greater detail. Here, we describe the use of a simple -mediated transient expression system adapted to and that could facilitate study of molecular and biochemical events in these species.