Top-down curing to construct self-retaining and moisture-pumping double-layered dressing with enhanced antibacterial, hemostatic, and wound healing performances.

Continuous microenvironment modulation is an ongoing challenge in wound dressing, which includes excessive exudate absorption, oxygen delivery, bacterial inhibition and angiogenesis. Herein, we developed an construction strategy to fabricate a self-retaining double-layered wound dressing, where the top layer precursor was composed of Ca-containing polyvinyl butyral (PVB) solution dispersed with hydroxypropyl methylcellulose (HPMC) particles, and the bottom one consisted of sodium alginate (Alg) solution blended with Ag-doped mesoporous bioactive glass powders (Ag-MBG). When in use, both precursors were simultaneously squeezed out from the twin nozzles connected to the individual chambers of a twin-chambered syringe, whereby Ca in the top layer rapidly migrated downwards to crosslink Alg in the bottom layer, leading to the formation of an Alg/Ag-MBG (AA) functional hydrogel for filling an irregular wound.

Operation of spinal sensorimotor circuits controlling phase durations during tied-belt and split-belt locomotion after a lateral thoracic hemisection.

Locomotion is controlled by spinal circuits that interact with supraspinal drives and sensory feedback from the limbs. These sensorimotor interactions are disrupted following spinal cord injury. The thoracic lateral hemisection represents an experimental model of an incomplete spinal cord injury, where connections between the brain and spinal cord are abolished on one side of the cord.

A narrative review on alcohol use in women: insight into the telescoping hypothesis from a biopsychosocial perspective.

There has been a dramatic rise in alcohol consumption and alcohol use disorder (AUD) among women. Recently, the field has made substantial progress toward better understanding sex and gender differences in AUD. This research has suggested accelerated progression to AUD and associated health consequences in women, a phenomenon referred to as "telescoping.

Proteomic Analysis Is Needed to Understand the Vulnerability of Sea Anemones to Climate Change.

Sea anemones play a crucial role in marine ecosystems. Recent studies have highlighted their physiological and ecological responses to thermal stress. Therefore, our objective was to perform a proteomic analysis of sea anemones in the Gulf of Mexico, subjected to thermal stress, to understand whether these organisms activate specific processes to resist increased temperature.

CircPRKD3-loaded exosomes concomitantly elicit tumor growth inhibition and glioblastoma microenvironment remodeling via inhibiting STAT3 signaling.

Background: Glioblastoma stem cells (GSCs) and their exosomes (exos) are involved in shaping the immune microenvironment, which is important for tumor invasion and recurrence. However, studies involving GSC-derived exosomal circular RNAs (GDE-circRNAs) in regulating tumor microenvironment (TME) remain unknown. Here, we comprehensively evaluated the significance of a novel immune-related GDE-circRNA in glioma microenvironment.

Draft genome assemblies of 35 bacteria isolated from hibernating arctic ground squirrels.

Arctic ground squirrels () hibernate for several months without eating or drinking yet suffer no disuse atrophy. We are investigating the potential contributions of gut microorganisms to host nitrogen homeostasis, and here, we describe the genome assemblies of 35 isolated bacteria collected from gastrointestinal material and sequenced using Nanopore technology.

Nanoencapsulated Optical Fiber-Based PEC Microelectrode: Highly Sensitive and Specific Detection of NT-proBNP and Its Implantable Performance.

Microelectrodes offer exceptional sensitivity, rapid response, and versatility, making them ideal for real-time detection and monitoring applications. Photoelectrochemical (PEC) sensors have shown great value in many fields due to their high sensitivity, fast response, and ease of operation. Nevertheless, conventional PEC sensing relies on cumbersome external light sources and bulky electrodes, hindering its miniaturization and implantation, thereby limiting its application in real-time disease monitoring.

Fullerene (C & C)-Meso-Tris-4-Carboxyphenyl Porphyrin Dyads Inhibit Entry of Wild-Type and Drug-Resistant HIV-1 Clades B and C.

The biological applications of noncationic porphyrin-fullerene (P-F) dyads as anti-HIV agents have been limited despite the established use of several cationic P-F dyads as anti-cancer photodynamic therapy (PDT) agents. This article explores the potential of amphiphilic non-cationic porphyrin-fullerene dyads as HIV-1 inhibitors under both PDT (light-treated) and non-PDT (dark) conditions. The amphiphilic P-F dyads, PBC and PBC, demonstrated enhanced efficacy in inhibiting the entry and production of HIV-1 (subtypes B and C).

Exploring the role of T cells in Alzheimer's and other neurodegenerative diseases: Emerging therapeutic insights from the T Cells in the Brain symposium.

This proceedings article summarizes the inaugural "T Cells in the Brain" symposium held at Columbia University. Experts gathered to explore the role of T cells in neurodegenerative diseases. Key topics included characterization of antigen-specific immune responses, T cell receptor (TCR) repertoire, microbial etiology in Alzheimer's disease (AD), and microglia-T cell crosstalk, with a focus on how T cells affect neuroinflammation and AD biomarkers like amyloid beta and tau.

Flow virometry: recent advancements, best practices, and future frontiers.

The imperative for developing robust tools to detect, analyze, and characterize viruses has become increasingly evident as they continue to threaten human health. In this review, we focus on recent advancements in studying human viruses with flow virometry (FV), an emerging technique that has gained considerable momentum over the past 5 years. These advancements include the application of FV in viral surface phenotyping, viral protein functionality, virus sorting, vaccine development, and diagnostics.

Investigating the threat to Sitka spruce from Ips typographus: discrimination and colonization of Britain's principal commercial conifer by a damaging forest pest.

Background: Ips typographus (L.), the eight-toothed spruce bark beetle (Coleoptera: Scolytinae), has devastated European Norway spruce (Picea abies) forests in recent years. For the first time, I.

A flagella-dependent jumbo phage controls rice seedling rot and steers toward reduced virulence in rice seedlings.

Bacteriophages (phages) are being investigated as potential biocontrol agents for the suppression of bacterial diseases in cultivated crops. Jumbo bacteriophages, which possess genomic DNA larger than 200 kbp, generally have a broader host range than other phages and therefore would be useful as biocontrol agents against a wide range of bacterial strains. Thus, the characterization of novel jumbo phages specific for agricultural pathogens would be of importance for the development of phage biocontrol strategies.

A Clinical Metaproteomics Workflow Implemented within Galaxy Bioinformatics Platform to Analyze Host-Microbiome Interactions Underlying Human Disease.

Clinical metaproteomics reveals host-microbiome interactions underlying diseases. However, challenges to this approach exist. In particular, the characterization of microbial proteins present in low abundance relative to host proteins is difficult.

Ameliorating salt stress in tomato by a top-down approach of acclimatizing the rhizosphere microbiome.

Soil salinization adversely impacts plant and soil health. While amendment with chemicals is not sustainable, the application of bioinoculants suffers from competition with indigenous microbes. Hence, microbiome-based rhizosphere engineering, focussing on acclimatization of rhizosphere microbiome under selection pressure to facilitate plant growth, exhibits promise.

Water Quality and Land Use Shape Bacterial Communities Across 621 Canadian Lakes.

Human activities such as agriculture and urban development are linked to water quality degradation. Canada represents a large and heterogeneous landscape of freshwater lakes, where variations in climate, geography and geology interact with land cover alteration to influence water quality differently across regions. In this study, we investigated the influence of water quality and land use on bacterial communities across 12 ecozones.

Integrated physiological, transcriptomic and metabolomic analyses reveal potential mechanisms of potato tuber dormancy release.

Regulating potato tuber dormancy is crucial for crop productivity and food security. We conducted the first comprehensive physiological, transcriptomic, and metabolomic investigations of two varieties of long and short dormant potato tubers in order to clarify the mechanisms of dormancy release. In the current study, three different dormant stages of UGT (ungerminated tubers), MGT (minimally germinated tubers), and GT (germinated tubers) were obtained by treatment with the germination promoter gibberellin A and the germination inhibitor chlorpropham.

Predicting the impact of targeted fence removal on connectivity in a migratory ecosystem.

Fencing is one of the most widely utilized tools for reducing human-wildlife conflict in agricultural landscapes. However, the increasing global footprint of fencing exceeds millions of kilometers and has unintended consequences for wildlife, including habitat fragmentation, movement restriction, entanglement, and mortality. Here, we present a novel and quantitative approach to prioritize fence removal within historic migratory pathways of white-bearded wildebeest (Connochaetes taurinus) across Kenya's Greater Masai Mara Ecosystem.

HOTAIR expression as a biomarker in glioblastoma multiforme: a comprehensive meta-analysis of current evidence.

Purpose: Glioblastoma multiforme (GBM) is an aggressive brain tumor. This meta-analysis investigates the association between HOTAIR expression levels and GBM.

Methods: We searched the literature for studies on HOTAIR expression in GBM patients.

Comparative pericarp biomechanics and germination physiology of Raphanus raphanistrum and Raphanus pugioniformis indehicent fruits.

The biomechanical, morphological and ecophysiological properties of plant seed/fruit structures are adaptations that support survival in unpredictable environments. High phenotypic variability of noxious and invasive weed species such as Raphanus raphanistrum (wild radish) allow diversification into new environmental niches. Dry indehiscent fruits (thick and lignified pericarp [fruit coat] enclosing seeds) have evolved many times independently.

Contrasting habitat associations and ecophysiological adaptations drive interspecific growth differences among Himalayan high-mountain plants.

Background And Aims: Understanding interspecific differences in plant growth rates and their internal and external drivers is key to predicting species responses to ongoing environmental changes. Annual growth rates vary among plants based on their ecological preferences, growth forms, ecophysiological adaptations, and evolutionary history. However, the relative importance of these factors remains unclear, particularly in high-mountain ecosystems experiencing rapid changes.

methylGrapher: genome-graph-based processing of DNA methylation data from whole genome bisulfite sequencing.

Genome graphs, including the recently released draft human pangenome graph, can represent the breadth of genetic diversity and thus transcend the limits of traditional linear reference genomes. However, there are no genome-graph-compatible tools for analyzing whole genome bisulfite sequencing (WGBS) data. To close this gap, we introduce methylGrapher, a tool tailored for accurate DNA methylation analysis by mapping WGBS data to a genome graph.

Genomic clustering tendency of transcription factors reflects phase-separated transcriptional condensates at super-enhancers.

Many transcription factors (TFs) have been shown to bind to super-enhancers, forming transcriptional condensates to activate transcription in various cellular systems. However, the genomic and epigenomic determinants of phase-separated transcriptional condensate formation remain poorly understood. Questions regarding which TFs tend to associate with transcriptional condensates and what factors influence their association are largely unanswered.

Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells.

Extracellular vesicles (EVs) from brain-seeking breast cancer cells (Br-EVs) breach the blood-brain barrier (BBB) via transcytosis and promote brain metastasis. Here, we defined the mechanisms by which Br-EVs modulate brain endothelial cell (BEC) dynamics to facilitate their BBB transcytosis. BEC treated with Br-EVs show significant downregulation of Rab11fip2, known to promote vesicle recycling to the plasma membrane and significant upregulation of Rab11fip3 and Rab11fip5, which support structural stability of the endosomal compartment and facilitate vesicle recycling and transcytosis, respectively.

Pathogenicity and phylogeny of Labyrinthula spp. isolated in Washington and Oregon, USA.

The class Labyrinthulomycetes constitutes a multitude of species found ubiquitously in the environment, and includes pathogens of corals, hard clams, turfgrasses, and seagrasses. Labyrinthula zosterae, the causative agent of seagrass wasting disease, has been associated with declines in seagrass coverage since the 1930s. However, pathogenic and nonpathogenic Labyrinthula spp.

Utilization of rice pasta by-products as the substrate for vinegar fermentation.

Unlabelled: The aim of this study was to utilize four rice pasta by-products (RPB), including white rice pasta, black jasmine rice pasta, red jasmine rice pasta and brown rice pasta, to ferment into four vinegars. The dynamic variations during fermentation and their quality were evaluated. During alcoholic fermentation, the saccharified substrates were fermented with for 12 days, resulting in an alcohol yield of approximately 10% (v/v) after 4 days of fermentation.