RBBP6 anchors pre-mRNA 3' end processing to nuclear speckles for efficient gene expression.

Pre-mRNA 3' processing is an integral step in mRNA biogenesis. However, where this process occurs in the nucleus remains unknown. Here, we demonstrate that nuclear speckles (NSs), membraneless organelles enriched with splicing factors, are major sites for pre-mRNA 3' processing in human cells.

DNA-binding affinity and specificity determine the phenotypic diversity in BCL11B-related disorders.

BCL11B is a Cys2-His2 zinc-finger (C2H2-ZnF) domain-containing, DNA-binding, transcription factor with established roles in the development of various organs and tissues, primarily the immune and nervous systems. BCL11B germline variants have been associated with a variety of developmental syndromes. However, genotype-phenotype correlations along with pathophysiologic mechanisms of selected variants mostly remain elusive.

A three-dimensional mouse liver organoid platform for assessing EDCs metabolites simulating liver metabolism.

Hepatic metabolism is an important process for evaluate the potential activity and toxicity of endocrine disrupting chemicals (EDCs) metabolites. Organization for Economic Co-operation and Development (OECD) has advocated the development of in vitro assays that mimic in vivo hepatic metabolism to eventually replace classical animal tests. In response to this need, we established a 3D mouse liver organoid (mLO) platform that mimics the animal model and is distinct from existing models.

Alterations of subcortical structural volume in pediatric bipolar disorder patients with and without psychotic symptoms.

Background: Pediatric bipolar disorder (PBD) with psychotic symptoms may predict more severe impairment in social functioning, but the underlying biological mechanisms remain unclear. The aim of this study was to investigate alterations in subcortical structural volume in PBD with and without psychotic symptoms.

Methods: We recruited 24 psychotic PBD (P-PBD) patients, 24 non-psychotic PBD (NP-PBD) patients, and 18 healthy controls (HCs).

Omics approaches: Role in acute myeloid leukemia biomarker discovery and therapy.

Acute myeloid leukemia (AML) is the most common acute leukemia in adults and has the highest fatality rate. Patients aged 65 and above exhibit the poorest prognosis, with a mere 30 % survival rate within one year. One important issue in optimizing outcomes for AML patients is their limited ability to predict responses to specific therapies, response duration, and likelihood of relapse.

Tetrabromobisphenol A, but not bisphenol A, disrupts plasma membrane homeostasis in myeloid cell models - A novel threat from an established persistent organic pollutant.

Bisphenol A (BPA, a plastic polymer component) and tetrabromobisphenol A (TBBPA, a brominated flame retardant) are industrial compounds and representative persistent organic pollutants (POPs) with similar chemical structure. We studied their impact on biological membrane dynamics, which is an emerging and understudied target for environmental contaminants, using a set of state-of-the-art methods. We found that exposure to TBBPA, but not to BPA, leads to disruption of biophysical homeostasis of the plasma membrane in myeloid cell lines HL-60, THP-1 and Mono Mac 6.

Chiral recognition of CIAC001 isomers in regulating pyruvate kinase M2 and mitigating neuroinflammation.

Chiral recognition plays a critical role in drug efficacy within biological systems. CIAC001, a cannabidiol (CBD) derivative that targets pyruvate kinase M2 (PKM2), has shown strong anti-neuroinflammatory and anti-morphine addiction effects. However, the chiral recognition of CIAC001, which contains multiple chiral centers, remains poorly understood.

Unlocking the phosphorus circularity potential of corn belt watersheds with biorefinery phosphorus recovery incentives.

As global phosphorus (P) stores rapidly decline, P fed algal blooms continue to threaten critical freshwater resources across the globe. In the Midwestern United States (US), particularly the Corn Belt, biorefineries could play a key role in addressing this issue. By recovering P from the byproducts of ethanol production these facilities could reduce the P content of distillers grain feed, thereby reducing P excreted in manures.

Nano-TiO impairs the health of crabs Charybdis japonica under warming conditions through waterborne and dietary exposures.

The widespread use of nano titanium dioxide (nano-TiO₂) poses ecological risks to marine ecosystems, especially when combined with ocean warming. However, most previous studies have only examined water-related exposures, leaving a gap in research on the impact of food transfer on organisms. In this work, the harmful impacts of nano-TiO on the Japanese swimming crab Charybdis japonica were studied through three scenarios: direct exposure (DE) of the crabs to warming and nano-TiO, indirect exposure (IE) via consumption of thick-shelled mussels Mytilus coruscus exposed to the same conditions, and combined exposure (CE), where crabs were directly subjected to warming and nano-TiO while feeding affected mussels.

A Lu-nucleotide coordination polymer-incorporated thermosensitive hydrogel with anti-inflammatory and chondroprotective capabilities for osteoarthritis treatment.

Osteoarthritis (OA) is a prevalent and debilitating condition characterized by cartilage destruction and inflammation. Traditional pharmacotherapies for OA are limited by their short-term efficacy and systemic side effects. Radiosynoviorthesis (RSO), involving intra-articular injection of radiopharmaceuticals, has shown promise for OA treatment but is hindered by the toxicity and rapid clearance of radioisotopes.

β-Catenin/c-Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations.

Ammonia a by-product of nitrogen containing molecules is detoxified by liver into non-toxic urea and glutamine. Impaired ammonia detoxification leads to hyperammonemia. Ammonia has a dual role on autophagy, it acts as inducer at low concentrations and as inhibitor at high concentrations.

Protocol to study neuronal membrane proteasome function in mouse peripheral sensory neurons.

Neuronal membrane proteasomes (NMPs) are expressed on a subset of somatosensory dorsal root ganglion (DRG) neurons and influence mechanical and pain sensitivity. Here, we present a protocol for studying NMP function in mouse peripheral sensory neurons. We describe steps for procuring and culturing primary DRG neurons.

Deciphering the colostral-immunity transfer: from mammary gland to neonates small intestine.

Colostrum, the initial mammary secretion produced by various mammals following birth, is a conduit for maternal immunity transfer in diverse mammalian species. Concurrently, many cellular processes are occurring in the neonatal small intestine to prepare it to receive molecular signals from a superfood essential for the neonate's health and development. During the prepartum colostrum secretion, the newborn intestine undergoes transient alterations in the intestinal barrier, primarily regulating immunoglobulin absorption.

Extracellular vesicles: essential agents in critical bone defect repair and therapeutic enhancement.

Bone serves as a fundamental structural component in the body, playing pivotal roles in support, protection, mineral supply, and hormonal regulation. However, critical-sized bone injuries have become increasingly prevalent, necessitating extensive medical interventions due to limitations in the body's capacity for self-repair. Traditional approaches, such as autografts, allografts, and xenografts, have yielded unsatisfactory results.

Dietary purslane (Portulaca oleracea L.) leaf powder maintains growth and intestinal health in Oreochromis niloticus under chronic water-borne cadmium exposure by strengthening the gut barriers, modulating the intestinal nutrient transporters, and relieving oxidative stress.

High cadmium (Cd) concentrations pose a threat to aquatic life globally. This study examined the efficiency of adding purslane (Portulaca oleracea L.) leaf powder (PLP) to Oreochromis niloticus diets on Cd's negative effects.

Human Brucellosis in the Rural and Urban Population of Pakistan: Seroprevalence, Risk Factors, and Clinical Manifestations.

Brucella spp. is the bacterium responsible for brucellosis, a zoonotic infection that affects humans. This disease poses significant health challenges and contributes to poverty, particularly in developing countries.

NFKB1 as a key player in Tumor biology: from mechanisms to therapeutic implications.

NFKB1, a core transcription factor critical in various biological process (BP), is increasingly studied for its role in tumors. This research combines literature reviews, meta-analyses, and bioinformatics to systematically explore NFKB1's involvement in tumor initiation and progression. A unique focus is placed on the NFKB1-94 ATTG promoter polymorphism, highlighting its association with cancer risk across diverse genetic models and ethnic groups, alongside comprehensive analysis of pan-cancer expression patterns and drug sensitivity.

Diagnostic value of glypican-1; a new marker differentiating pulmonary squamous cell carcinoma from adenocarcinoma: immunohistochemical study on Egyptian series.

Lung cancer is one of the major causes of cancer morbidity and mortality. Subtyping of non-small cell lung cancer is necessary owing to different treatment options. This study is to evaluate the value of immunohistochemical expression of glypican-1 in the diagnosis of lung squamous cell carcinoma (SCC).

The cold tolerance of the terrestrial slug, Ambigolimax valentianus.

Terrestrial molluscs living in temperate and polar environments must contend with cold winter temperatures. However, the physiological mechanisms underlying the survival of terrestrial molluscs in cold environments and the strategies employed by them are poorly understood. Here we investigated the cold tolerance of Ambigolimax valentianus, an invasive, terrestrial slug that has established populations in Japan, Canada, and Europe.

Harnessing IL-27: challenges and potential in cancer immunotherapy.

IL-27 is structurally an immune-enhancing and pleiotropic two-chain cytokine associated with IL-12 and IL-6 families. IL-27 contains two subunits, namely IL-27p28 and EBI3. A heterodimer receptor of IL-27, composed of IL27Rα (WSX1) and IL6ST (gp130) chains, mediates the IL-27 function following the activation of STAT1 and STAT3 signaling pathways.

The Society of Thoracic Surgeons (STS) Clinical Practice Guideline on Surgical Management of Oligometastatic Non-small Cell Lung Cancer.

Background: The use of local consolidative therapy (LCT) in patients with oligometastatic non-small cell lung cancer (NSCLC) is rapidly evolving, with a preponderance of data supporting the benefits of such therapeutic approaches incorporating pulmonary resection for appropriately selected candidates. However, practices vary widely institutionally and regionally, and evidence-based guidelines are lacking.

Methods: The Society of Thoracic Surgeons assembled a panel of thoracic surgical oncologists to evaluate and synthesize the available evidence regarding the role of pulmonary resection as LCT.

Identification and Characterization of a New Thermophilic κ-Carrageenan Sulfatase.

Carrageenans are sulfated polysaccharides found in the cell wall of certain red seaweeds. They are widely used in the food industry for their gelling and stabilizing properties. In nature, carrageenans undergo enzymatic modification and degradation by marine organisms.

GDBr: genomic signature interpretation tool for DNA double-strand break repair mechanisms.

Large genetic variants can be generated via homologous recombination (HR), such as polymerase theta-mediated end joining (TMEJ) or single-strand annealing (SSA). Given that these HR-based mechanisms leave specific genomic signatures, we developed GDBr, a genomic signature interpretation tool for DNA double-strand break repair mechanisms using high-quality genome assemblies. We applied GDBr to a draft human pangenome reference.

inDrops-2: a flexible, versatile and cost-efficient droplet microfluidic approach for high-throughput scRNA-seq of fresh and preserved clinical samples.

The expansion of single-cell analytical techniques has empowered the exploration of diverse biological questions at the individual cells. Droplet-based single-cell RNA sequencing (scRNA-seq) methods have been particularly widely used due to their high-throughput capabilities and small reaction volumes. While commercial systems have contributed to the widespread adoption of droplet-based scRNA-seq, their relatively high cost limits the ability to profile large numbers of cells and samples.

Livestock shifts lepidopteran herbivore community due to intraguild elimination in Mediterranean agroforestry systems.

Livestock grazing and trampling have been shown to reduce arthropod populations. Among arthropods, defoliating lepidopterans are particularly important for their impact on trees, the keystone structures of agroforestry systems. This study investigates the impact of livestock on the community of defoliating lepidopterans in agroforestry systems.