DMSO tolerant NAD(P)H recycler enzyme from a pathogenic bacterium, PC543: effect of N-/C-terminal His Tag extension on protein solubility and activity.

NAD(P) dependent formate dehydrogenase (FDH) is an oxidoreductase used as a biocatalyst to regenerate NAD(P)H in reductase-mediated chiral synthesis reactions. Solvent stability and the need to reduce NADP to NADPH, due to the high cost of NADPH, are required features in the industrial usage of FDHs. Therefore, we aimed to identify a novel, robust NADP dependent FDH and evaluate the effect of N- and C- terminus His tag extensions on protein solubility and activity.

Comparative interactomics for virus-human protein-protein interactions: DNA viruses versus RNA viruses.

Viruses are obligatory intracellular pathogens and completely depend on their hosts for survival and reproduction. The strategies adopted by viruses to exploit host cell processes and to evade host immune systems during infections may differ largely with the type of the viral genetic material. An improved understanding of these viral infection mechanisms is only possible through a better understanding of the pathogen-host interactions (PHIs) that enable viruses to enter into the host cells and manipulate the cellular mechanisms to their own advantage.