Nestin Forms a Flexible Cytoskeleton by Means of a Huge Tail Domain That Is Reversibly Stretched and Contracted by Weak Forces.

Nestin is a type VI intermediate filament protein and a well-known neural stem cell marker. It is also expressed in high-grade cancer cells, forming copolymerized filaments with vimentin. We previously showed that nestin inhibits the binding of vimentin's tail domain to actin filaments (AFs) by steric hindrance through its large nestin tail domain (NTD), thereby increasing three-dimensional cytoskeleton network mobility, enhancing cell flexibility, and promoting cancer progression.

Release Profile and Antibacterial Activity of Essential Oil-Incorporated, Optimally Stabilized Type I Collagen Hydrogels.

Antimicrobial resistance is one of the drastically increasing major global health threats due to the misuse and overuse of antibiotics as traditional antimicrobial agents, which render urgent the need for alternative and safer antimicrobial agents, such as essential oils (EOs). Although the strong antimicrobial activity of various EOs has already been studied and revealed, their characteristic high sensitivity and volatility drives the need towards a more efficient drug administration method via a biomaterial system. Herein, the potential of EO incorporated in functionalized antibacterial collagen hydrogels was investigated.

Genetic variation in zona pellucida-3 (ZP3) gene and its association with litter size variation in Kari sheep.

Variation in litter size (LS) in sheep is linked to genetic factors, including the Zona pellucida-3 (ZP3) gene, which plays a role in ovine reproductive processes. This study examined the association between ZP3 gene variations and LS in Kari sheep. Two groups of 160 Kari ewes were analysed: one consistently producing singletons and another producing twins, with occasional triplets.

A chromosome-scale genome assembly of the pioneer plant Stylosanthes angustifolia: insights into genome evolution and drought adaptation.

Background: Drought is a major limiting factor for plant survival and crop productivity. Stylosanthes angustifolia, a pioneer plant, exhibits remarkable drought tolerance, yet the molecular mechanisms driving its drought resistance remain largely unexplored.

Results: We present a chromosome-scale reference genome of S.

Effects of Banana Flour on Some Physicochemical, Textural, Bioactive, and Sensory Properties of Gluten-Free Cookie.

The present study aimed to investigate the effects of banana flours obtained from different banana cultivars (Grand Nain (GN), Azman (AZ), and Erdemli (ER)) on some basic physicochemical, bioactive, textural, and sensory properties of the gluten-free cookie samples by the simplex lattice mixture design approach. Incorporating banana powder into cookie samples resulted in significant changes in the parameters studied. The banana flours' total dietary fiber and total starch levels ranged between 1.

Investigating the Effect of Syringe Infiltration on (Tobacco).

Plant infiltration techniques, particularly agroinfiltration, have transformed plant science and biotechnology by enabling transient gene expression for genetic engineering of plants or genomic studies. Recently, the use of infiltration has expanded to introduce nanomaterials and polymers in plants to enable nonnative functionalities. Despite its wide use, the impact of the infiltration process on plant physiology needs to be better understood.

Odyssey of environmental and microbial interventions in maize crop improvement.

Maize () is India's third-largest grain crop, serving as a primary food source for at least 30% of the population and sustaining 900 million impoverished people globally. The growing human population has led to an increasing demand for maize grains. However, maize cultivation faces significant challenges due to a variety of environmental factors, including both biotic and abiotic stresses.

Genome-wide analysis of sugar transporter gene family in and , expression profiling and identification of transcription factors.

Sugar, the primary product of photosynthesis, is a vital requirement for cell activities. Allocation of sugar from source to sink tissues is facilitated by sugar transporters (ST). These STs belong to the Major Facilitator Superfamily (MFS), the largest family of STs in plants.

Novel silver nanoparticle-based biomaterials for combating biofilms.

Background: is a significant nosocomial pathogen that has developed resistance to multiple antibiotics, often forming biofilms that enhance its virulence. This study investigated the efficacy of a novel nanoformulation, AgNPs@chitosan-NaF, in combating biofilms.

Methods: Antimicrobial susceptibility testing was performed to assess the antibiotic resistance profile of isolates.

Preparation and evaluation of MSR-1 bioinoculant on the growth and productivity of (L.) R. Wilczek.

Unlabelled: Recently, there has been a growing interest in the application of beneficial microorganisms to enhance crop performance.  (MSR-1) are spiral-shaped, gram-negative bacteria that exhibit magnetotaxis with the help of magnetosomes (iron oxide or iron sulphide). They have exhibited biomedical and environmental applications; however, the agricultural utilization of these strains is yet to be explored.

Protective Potential of Strains and Their Mixture on Inflammatory Bowel Disease via Regulating Gut Microbiota in Mice.

The aim of this study is to investigate the protective potential of IM57, IR51, and IR62 strains, isolated from infant feces, and their mixture against inflammatory bowel disease (IBD). The strains exhibited robust antioxidant activities and anti-inflammatory properties in RAW 264.7 cells.

Inhibitory Effects of Compounds Isolated from L. (Noni) Seeds against Particulate Matter-Induced Injury.

L. (noni) is native to the tropical and semitropical areas and has been commercially available in health food stores and chain grocery stores specializing in natural foods, recently. Noni seeds are discarded as waste products through the industrial production of noni juice even though their bioactivity components might be a potential source of functional foods.

Comprehensive Analysis of the Fourteen Complete Genome Sequences of Isolated from Species.

Endosymbionts are important for insect species as they provide essential substances to the host. Due to the technical advance of NGS technology and assemblers, many endosymbionts bacterial genomes are available now. Here, we analysed fourteen endosymbiont bacterial genomes of genius, one of notorious pest species.

Bibliometric Analysis of Probiotic Bacillus in Food Science: Evolution of Research Trends and Systematic Evaluation.

With the in-depth and comprehensive research on probiotic Bacillus, it has become a hot topic in food science. However, the current status of research using bibliometric analysis to assess the application of probiotic Bacillus in food science has not been comprehensively reviewed. The Web of Science (WOS) database was used in this review's bibliometric analysis to determine the hotspots for research as well as the extent of completed experiments.

Integrative bioinformatics approaches reveal key hub genes in cyanobacteria: insights from Synechocystis sp. PCC 6803 and Geminocystis sp. NIES-3708 under abiotic stress conditions.

Background: Cyanobacteria, particularly Synechocystis sp. PCC 6803, serve as model organisms for studying acclimation strategies that enable adaptation to various environmental stresses. Understanding the molecular mechanisms underlying these adaptations provides insight into how cells adjust gene expression in response to challenging conditions.

Genetic analysis and heterosis breeding of seed yield and yieldattributing traits in Indian mustard (Brassica juncea (L.) Czern & Coss.).

This study aimed to assess the genetic basis and combining ability of 10 morphological traits in Indian mustard. The experiment involved eight parent lines and 28 crosses derived from a half-diallel mating design. Combining ability analysis is vital for identifying parents and hybrids with favorable genetic effects to enhance breeding efficiency.

An exploration of RSM, ANN, and ANFIS models for methylene blue dye adsorption using Oryza sativa straw biomass: a comparative approach.

This study focused on simulating the adsorption-based separation of Methylene Blue (MB) dye utilising Oryza sativa straw biomass (OSSB). Three distinct modelling approaches were employed: artificial neural networks (ANN), adaptive neuro-fuzzy inference systems (ANFIS), and response surface methodology (RSM). To evaluate the adsorbent's potential, assessments were conducted using Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM).

Urea enhances the yield and quality of bio-oil produced from corncob through pyrolysis.

As global demand for fossil fuels rises amidst depleting reserves and environmental concerns, exploring sustainable and renewable energy sources has become imperative. This study investigated the pyrolysis of corncob, a widely available agricultural waste, using urea as a catalyst to enhance bio-oil production. The aim was to determine the optimum urea concentration and pyrolysis temperature for bio-oil yield from corncob.

Enhancement of the production of terpenoid and flavonoid secondary metabolites in the ground and aerial parts of licorice composite plant in a hydroponic system.

Hairy roots mediated by Agrobacterium rhizogenes can be obtained from the composite plants (plants with hairy roots and untransformed aerial parts) by ex vitro method. Composite plants can produce higher amounts of secondary metabolites by merging hydroponic systems. This provides a stable condition for composite plants, in which various metabolites are produced in different parts.

Hyperplex PCR enables highly multiplexed analysis of point mutations in wastewater: Long-term SARS-CoV-2 variant surveillance in Sweden as a case study.

Wastewater-based surveillance (WBS) allows the analysis of pathogens, chemicals or other biomarkers in wastewater to derive unbiased epidemiological information at population scale. After re-gaining attention during the SARS-CoV-2 pandemic, the field holds promise as a surveillance and early warning system by tracking emerging pathogens with pandemic potential. Expanding the current toolbox of analytical techniques for wastewater analysis, we explored the use of Hyperplex PCR (hpPCR) to analyse SARS-CoV-2 mutations in wastewater samples collected weekly in up to 22 sites across Sweden between October 2022 and December 2023.

Unelongated Stems are an Active Nitrogen-Fixing Site in Rice Stems Supported by Both Sugar and Methane Under Low Nitrogen Conditions.

Enhancing nitrogen (N) fixation in rice plants can reduce N fertilizer application and contribute to sustainable rice production, particularly under low-N conditions. However, detailed microbial and metabolic characterization of N fixation in rice stems, unlike in the well-studied roots, has not been investigated. Therefore, the aim of this study was to determine the active N-fixing sites, their diazotroph communities, and the usability of possible carbon sources in stems compared with roots.

Unveiling remarkable bacterial diversity trapped by cowpea (Vigna unguiculata) nodules inoculated with soils from indigenous lands in Central-Western Brazil.

Cowpea (Vigna unguiculata) is recognized as a promiscuous legume in its symbiotic relationships with rhizobia, capable of forming associations with a wide range of bacterial species. Our study focused on assessing the diversity of bacterial strains present in cowpea nodules when inoculated with soils from six indigenous lands of Mato Grosso do Sul state, Central-Western Brazil, comprising the Cerrado and the Pantanal biomes, which are known for their rich diversity. The DNA profiles (BOX-PCR) of 89 strains indicated great genetic diversity, with 20 groups and 23 strains occupying single positions, and all strains grouped at a final similarity level of only 25%.

A Cost-Effective Nonaqueous Reversed-Phase High-Performance Liquid Chromatography Method to Measure Vitamin D3 in Hen's Egg Yolk.

The objective of this study is to develop an HPLC-UV method for the cost-effective and quantitative determination of vitamin D3 in food, even in the presence of vitamin D2, with a specific focus on egg yolk. During method development, the performance of three stationary phases in resolving the peak of vitamin D2 from that of vitamin D3 was investigated. The physicochemical properties of these phases differed particularly in the extent of hydrophobicity and silanophilic activity, including a GraceSmart RP C18 column without silanol endcapping, a Robusta RP C18 column with silanol endcapping, and a Waters Xbridge RP C18 column with ethylene-bridged hybrid (BEH) particle technology.

Genotypic variability in stress responses of Sorghum bicolor under drought and salinity conditions.

Introduction: Sorghum bicolor: widely cultivated in Asia and Africa, faces increasing challenges from climate change, specifically from abiotic stresses like drought and salinity. This study evaluates how different sorghum genotypes respond to separate and combined stresses of drought and salinity.

Methods: Carried out with three replications using a randomized complete block design, the experiment measured biochemical and physiological parameters, including stomatal conductance, chlorophyll content, and antioxidant enzyme activities.