Prospective Head-to-Head Comparison of F-PSMA PET/CT and F-NaF PET/CT for Assessing Bone Metastases in 160 Patients with Newly Diagnosed High-Risk Prostate Cancer.

Prostate-specific membrane antigen (PSMA) PET/CT is increasingly used for primary staging in prostate cancer (PC), mainly because of its improved accuracy in detecting lymph node metastases compared with conventional imaging. However, the diagnostic benefit of PSMA PET/CT for detecting bone metastases is less well established. This study compares the diagnostic accuracy of F-PSMA PET/CT and F-NaF PET/CT for detecting bone metastases in patients newly diagnosed with PC.

Efficacy and safety of the PHIL embolic agent in the treatment of intracranial dural arteriovenous fistulas: results of the PHIL-dAVF study.

Background And Purpose: Embolization is the first-line treatment for dural arteriovenous fistulas (dAVF). The precipitating hydrophobic injectable liquid (PHIL) embolic agent is a non-adhesive copolymer with specific features and endovascular behavior. This study assessed its safety and efficacy in a prospective real-life cohort.

Modeling the effects of heat stress on production and enteric methane emission in high-yielding dairy herds.

Given global warming and the growing dairy population, heat stress in dairy herds is of increasing concern. During heat stress, dairy cows suffer from compromised productivity and animal welfare in terms of reduced feed intake and milk production, decreased reproductive performance, and generally increased risk of health problems. These effects and their interactions are complex and are usually quantified separately, and thereby a comprehensive understanding of the herd-level performance is missing.

Lack of correlation between school reopening and trends in adult COVID-19 hospitalisations and death rates during the Delta and early Omicron periods: an ecological analysis of five countries.

Objectives: In this ecological study, we describe SARS-CoV-2 case incidence for school age and adult populations, COVID-19 hospitalisation and death rates during Delta and the early Omicron periods, before and after schools reopened in five countries.

Methods: Data were extracted from government websites. Cases and COVID-19 hospitalisation and death incidence rates were calculated during the Delta and early Omicron periods in Australia, Canada, Denmark, Finland and the United Kingdom, for two weeks preceding and six weeks after schools reopened.

The microalgal sector in Europe: towards a sustainable bioeconomy.

Microalgae are a diverse group of photosynthetic microorganisms that can be exploited to produce sustainable food and feed products, alleviate environmental pollution, or sequester CO to mitigate climate change, among other uses. To optimize resource use and integrate industrial waste streams, it is essential to consider factors such as the biology and cultivation parameters of the microalgal species and strains, as well as the cultivation system and processing technologies employed. This paper reviews the main commercial applications of microalgae (including cyanobacteria) and examines the biological and biotechnological aspects critical to the sustainable processing of microalgal biomass and its derived compounds.

Clinical characteristics, management, and malignancy rate of oral lichen planus: a European multicenter study.

Introduction: Oral lichen planus (OLP) is a chronic inflammatory mucocutaneous condition that includes a spectrum of oral clinical manifestations ranging from mild painless white lesions to painful erosions and ulcers. The purpose of this European multicenter study is to describe the general characteristics of OLP lesions, the clinical and histopathological diagnosis, and the management of OLP at different European Oral Medicine and Maxillofacial Surgery centers, in order to minimize selections biases and provide information about the current trends in the treatment of OLP across Europe.

Materials And Methods: Data and histopathological records of patients with OLP were retrospectives revised and only those patients that fulfilled the diagnostic criteria from the 2016 position paper by American Academy of Oral and Maxillofacial Pathology were included.

CIFR (Clone-Integrate-Flip-out-Repeat): a toolset for iterative genome and pathway engineering of Gram-negative bacteria.

Advanced genome engineering enables precise and customizable modifications of bacterial species, and toolsets that exhibit broad-host compatibility are particularly valued owing to their portability. Tn5 transposon vectors have been widely used to establish random integrations of desired DNA sequences into bacterial genomes. However, the iteration of the procedure remains challenging because of the limited availability and reusability of selection markers.

Prognostic value of CD163 macrophages in solid tumor malignancies: A scoping review.

Tumor-associated macrophages (TAMs) play crucial roles in development and progression of malignant diseases. Notably, CD163 TAMs likely perform specific pro-tumorigenic functions, suggesting that this subset may serve as both prognostic biomarkers and targets for future anti-cancer therapy. We conducted a scoping review to map the current knowledge on the prognostic role of CD163 TAMs in the five most lethal cancers worldwide: Lung, colorectal, gastric, liver, and breast cancer.

Insulin amyloid morphology is encoded in H-bonds and electrostatics interactions ruling protein phase separation.

Ion-protein interactions regulate biological processes and are the basis of key strategies of modulating protein phase diagrams and stability in drug development. Here, we report the mechanisms by which H-bonds and electrostatic interactions in ion-protein systems determine phase separation and amyloid formation. Using microscopy, small-angle X-ray scattering, circular dichroism and atomistic molecular dynamics (MD) simulations, we found that anions specifically interacting with insulin induced phase separation by neutralising the protein charge and forming H-bond bridges between insulin molecules.

What does it mean to be a 'foster parent'? -exploring Foster parent narratives using ideal-type analysis.

Objective: Foster care services in Denmark, as in many other countries, face challenges with recruitment, assessment, and retention of foster families. It is essential to understand how foster parents understand their role and how this might relate to child outcomes. This paper develops a typology of foster parent types through an ideal-type analysis of interviews with foster parents.

Reduced healthcare costs for patients with screen-detected colorectal cancer: A Danish nationwide cohort study.

Objectives: This Danish nationwide retrospective register-based cohort study investigated healthcare costs for patients with screen-detected colorectal cancer (SD-CRC) compared to non-screen-detected CRC (NSD-CRC).

Study Design: Nationwide cohort study.

Methods: Quarterly healthcare costs including costs of hospital care, out-of-hospital medication, and primary sector contacts were compared between the two groups from two years before diagnosis of CRC until two years after.

Metabolizable energy and digestible nutrient values of protease-supplemented field pea for broiler chicken.

A study was conducted to determine the effects of protease supplementation of field pea (in comparison with soybean meal; SBM) for broilers on apparent metabolizable energy (AMEn) and standardized ileal digestibility (SID) of amino acids (AA). One hundred and forty broiler chicks were divided into 35 groups of 4 birds/group and fed 5 diets in a completely randomized design (7 groups/diet) from 14 to 21 d of age. The diets were cornstarch-based containing SBM or field pea as the sole protein source without or with protease (ProSparity 250; CBS Bio Platforms, Calgary, AB, Canada) in 2 × 2 factorial arrangement, and N-free diet.

Artificially tagging tumors with nano-aluminum adjuvant-tethered antigen mRNA recruits and activates antigen-specific cytotoxic T cells for enhanced cancer immunotherapy.

T cell therapy for solid tumors faces significant challenges due to the immune off-target attack caused by the loss of tumor surface antigens and inactivation in acidic tumor microenvironment (TME). Herein, we developed a bifunctional immunomodulator (MO@NAL) by loading ovalbumin (OVA; model antigen) mRNA (mOVA) onto lysozyme-coated layered double hydroxide nano-aluminum adjuvant (NA). The NA's inherent alkalinity effectively neutralizes the excess acid within the TME and suppresses regulatory T cells, creating a favorable microenvironment to enhance cytotoxic T cell infiltration and activation in tumors.

Protein-Polymer Conjugates as Biocompatible and Recyclable ATRP Catalysts.

Atom transfer radical polymerization (ATRP) is a leading method for creating polymers with precise control over molecular weight, yet its reliance on metal catalysts limits its application in metal-sensitive and environmental contexts. Addressing these limitations, we have developed a recyclable, biocompatible, robust, and tunable ATRP catalyst composed of a protein-polymer-copper conjugate, synthesized by polymerizing an -proline-based monomer onto bovine serum albumin and complexing with Cu(II). The use of this conjugate catalyst maintains ATRP's precision while ensuring biocompatibility with both and HEK 293 cells, and its high molecular weight allows for easy recycling through dialysis.

PepFuNN: Novo Nordisk Open-Source Toolkit to Enable Peptide in Silico Analysis.

We present PepFuNN, a new open-source version of the PepFun package with functions to study the chemical space of peptide libraries and perform structure-activity relationship analyses. PepFuNN is a Python package comprising five modules to study peptides with natural amino acids and, in some cases, sequences with non-natural amino acids based on the availability of a public monomer dictionary. The modules allow calculating physicochemical properties, performing similarity analysis using different peptide representations, clustering peptides using molecular fingerprints or calculated descriptors, designing peptide libraries based on specific requirements, and a module dedicated to extracting matched pairs from experimental campaigns to guide the selection of the most relevant mutations in design new rounds.

Evaluation of Health Education Based on Pictograms and Video Sequences in the Prevention of Hand Eczema Among Hospital Cleaners: A Cluster-Randomised Controlled Trial With 24 Weeks Follow-Up.

Background: Hand eczema (HE) is common among hospital cleaners, yet no specific prevention programme exists for this group in Denmark.

Objectives: To evaluate the effectiveness of visual aids (pictograms and video scenes) based on evidence-based skin care and protection recommendations on HE outcomes, focusing on disease severity, point prevalence and skin care and protection knowledge.

Methods: A cluster-randomised controlled trial was conducted in professional cleaners from three Danish hospitals.

Comparing the OurRelationship Program and Bibliotherapy for Parents Experiencing Couple Distress: A Randomized Pilot.

The OurRelationship Program is an 8-h web-based program for distressed couples adapted from Integrative Behavioral Couple Therapy. In this pilot randomized controlled trial, we aimed to replicate results from the founders and test feasibility criteria for program implementation across cultures. We randomized 39 Danish, opposite-sex, distressed, parenting couples to either the OurRelationship program or self-guided bibliotherapy (a self-help book with a reading guide).

Prophylactic Peanut Allergen Ara h 6 Sublingual Immunotherapy Drives Expansion of FoxP3Helios Regulatory T Cells in the Absence of Allergen-Specific IgA.

Insights into the underlying immunological mechanisms of prophylactic sublingual immunotherapy (SLIT) may support the development of new strategies for improved prevention and treatment of food allergy. Here, we investigated the humoral, regulatory and sublingual tissue immune response to prophylactic SLIT administration of a single purified peanut allergen in Brown Norway (BN) rats. BN rats received daily sublingual administration of peanut allergen Ara h 6 for three weeks.

Establishing reference curves for vital tissue perfusion using quantitative near-infrared fluorescence imaging with indocyanine green.

Purpose: Assessment of tissue perfusion using near-infrared fluorescence (NIR) with indocyanine green (ICG) is gaining popularity, however reliable and objective interpretation remains a challenge. Therefore, this study aimed to establish reference curves for vital tissue perfusion across target tissues using this imaging modality.

Methods: Data from five prospective study cohorts conducted in three Dutch academic medical centres between December 2018 and June 2023 was included.

Clinical experience with denosumab discontinuation.

Unlabelled: In patients receiving long-term treatment with denosumab, denosumab discontinuation via sequential treatment with zoledronate, resulted in a minor decrease in bone mass density (BMD) of 0-2.5% within the first year and stabile BMD in the second year, thus showing that repeated treatments with zoledronate limit the loss of BMD, when discontinuing denosumab.

Purpose: Discontinuing denosumab (DMAb) rapidly decreases bone mineral density (BMD) and increases the risk of multiple vertebral fractures.