Development of antibody to virulence factor flagellin and its evaluation in screening Ralstonia pseudosolanacearum.

The bacterial wilt disease caused by Ralstonia pseudosolanacearum presents a notable economic risk to a variety of crucial crops worldwide. During preliminary isolation of this phytopathogen, several colonies of other saprophytic bacteria may be mistaken with it. So, the present study aims to address this issue by proposing the application of immunogenic proteins, particularly flagellin (FliC), to enable a rapid and early identification of bacterial wilt.

Recent advances in immuno-based methods for the detection of Ralstonia solanacearum.

Ralstonia solanacearum (RS) is a widely recognized phytopathogenic bacterium which is responsible for causing devastating losses in a wide range of economically significant crops. Timely and accurate detection of this pathogen is pivotal to implementing effective disease management strategies and preventing crop losses. This review provides a comprehensive overview of recent advances in immuno-based detection methods for RS.

The potential of radiation sterilized and banked tissue allografts for management of nuclear casualties.

Processed and radiation sterilized allograft tissues that can be banked for use on demand are a precious therapeutic resource for the repair or reconstruction of damaged or injured tissues. Skin dressings or skin substitutes like allograft skin, amniotic membrane and bioengineered skin can be used for the treatment of thermal burns and radiation induced skin injuries. Bone grafts can be employed for repairing fracture defects, filling in destroyed regions of bone, and treatment of spinal and joint injuries.

L. rhizobacteria exhibit diversified cellulase and pectinase activities.

L. provides an enormous range of medicinal and nutritional benefits. The significant abilities of this plant to survive in Himalayan high altitudes enticed our study to investigate its rhizosphere.

Canine babesiosis among working dogs of organised kennels in India: A comprehensive haematological, biochemical, clinicopathological and molecular epidemiological multiregional study.

Canine babesiosis is a serious disease among tick-borne haemoprotozoan diseases, globally. The present study was envisaged for carrying out thorough investigation of the disease among working dogs of organised kennels situated in different agro-climatic zones of India as comprehensive understanding of the disease from this country was pertinently lacking. During the study period of three years (2012-2014), 330 dogs suspected for babesiosis were examined for clinicopathology by their physical examination, haematological and biochemical parameters estimation, while the detection of apicomplexan parasites was confirmed by using various diagnostic techniques i.

Systems biology of seeds: decoding the secret of biochemical seed factories for nutritional security.

Seeds serve as biochemical factories of nutrition, processing, bio-energy and storage related important bio-molecules and act as a delivery system to transmit the genetic information to the next generation. The research pertaining towards delineating the complex system of regulation of genes and pathways related to seed biology and nutrient partitioning is still under infancy. To understand these, it is important to know the genes and pathway(s) involved in the homeostasis of bio-molecules.

Bioactive potential of Indian stinging plants leaf extract against pathogenic fungi.

Background & methods We investigated the in vitro antioxidant and antifungal activity by agar disc diffusion assay of leaf extract of some stinging plants namely, Urtica dioica L., Tragia involucrate L., Carduus nutans L.

Abortions in an organized dairy farm from North India reveal the possibility of breed susceptibility to Bovine Brucellosis.

The present study was undertaken over a three year period (2012-2014) in an organized dairy farm located in North India to ascertain as the putative cause of abortion. The dairy farm maintained cattle of Frieswal, Crossbred and Sahiwal breeds and followed calf-hood vaccination with Strain 19 live vaccine in all the heifers. Even with the recommended vaccination schedule and good managemental practices in place, 88 cases of abortions clinically suspected of bovine brucellosis (40 from Frieswal breed, 17 from Crossbred cattle and 31 from Sahiwal breed) were reported from this farm.

Canine Monocytic Ehrlichiosis among working dogs of organised kennels in India: A comprehensive analyses of clinico-pathology, serological and molecular epidemiological approach.

Canine Monocytic Ehrlichiosis (CME) is a serious tick-borne rickettsial disease affecting canine populations globally. Besides few reports from stray and pet dogs from localised geographical regions (cities/towns/small states), a comprehensive study on prevalence of Ehrlichia canis (E. canis) among working dogs from different geo-climatic zones of India was pertinently lacking.

Enhancement of Neuromuscular Activity by Natural Specimens and Cultured Mycelia of Cordyceps sinensis in Mice.

The present study was aimed to evaluate the effect of natural specimen and laboratory cultured mycelia of Cordyceps sinensis on neuromuscular activity in mice. The powder of natural specimen and laboratory cultured Cordyceps sinensis was orally administered at the dose rate of 100, 300 and 500 mg/kg for 30 days. Natural specimen and in vitro propagated Cordyceps sinensis showed significant (P<0.

Female plants of Hippophae salicifolia D. Don are more responsive to cold stress than male plants.

Hippophae salicifolia D. Don (Seabuckthorn) grows in stressful environment of high altitude under conditions of low temperature and low availability of water. We have studied gender based differences in physiochemical response to cold stress in male and female plants of Seabuckthorn.

Cloning and characterization of GPAT gene from Lepidium latifolium L.: a step towards translational research in agri-genomics for food and fuel.

Glycerol-3-phosphate acyltransferase (GPAT) catalyzes first and the rate limiting step in glycerolipid synthesis pathway, which in turn contribute to stabilization of plasma membrane structure and oil lipid synthesis in plant cells. Here, we report cloning and characterization of GPAT gene from Lepidium latifolium (LlaGPAT). The cDNA sequence (1,615 bp) of LlaGPAT gene consisted of 1,113 bp ORF encoding a protein of 370 aa residues, with deduced mass of 41.

DRE-binding transcription factor gene (LlaDREB1b) is regulated by various abiotic stresses in Lepidium latifolium L.

We have isolated and in silico characterized a cold-induced LlaDREB1b encoding a putative DRE-binding transcription factor from Lepidium latifolium. Its cDNA (JN214345) sequence (998 bp) consisted of a 642 bp ORF, 168 and 188 bp of 5' and 3' UTR regions, respectively, encoding a protein of 213 aa with deduced molecular mass 23.85 kDa and pI of 4.

Evaluation of antioxidant activity of two important memory enhancing medicinal plants Baccopa monnieri and Centella asiatica.

Background: Free radicals or highly reactive oxygen species are capable of inducing oxidative damage to human body. Antioxidants are the compounds which terminate the attack of reactive species and reduce the risk of diseases. Both Baccopa monnieri and Centella asiatica are used in treatment of brain disorders in humans and have almost similar effects.

Breaking seed dormancy in Hippophae salicifolia, a high value medicinal plant.

Seed dormancy is an important limiting factor in exploitation of an economically important species to its fullest. Hippophae salicifolia D. Don (seabuckthorn), a rich source of medicinal metabolites shows both exogenous and endogenous dormancy.