Characterizing zoo-housed Bactrian camel (Camelus bactrianus) reproduction using gonadal steroid metabolite analysis in feces.

Millions of people globally depend on camelids, which demands an increased knowledge of their reproduction. We used zoo-housed Bactrian camels (Camelus bactrianus) to better understand camelid reproductive physiology. Our specific objectives were to: 1) validate the use of fecal hormone metabolite analysis to characterize camel reproductive physiology during sexual maturity and pregnancy; and 2) determine the influence of season on male and female reproduction.

RECOMBITEK CANINE DISTEMPER VACCINE AS AN ALTERNATIVE FOR PUREVAX DISTEMPER VACCINE IN ENDANGERED BLACK-FOOTED FERRETS ().

Black-footed ferrets () are an endangered species in North America that are highly sensitive to canine distemper virus (CDV) infections and any exposure could be devastating to species recovery. The U.S.

Using dermal glucocorticoids to determine the effects of disease and environment on the critically endangered Wyoming toad.

Amphibian populations are declining worldwide, and increased exposure to environmental stressors, including global climate change and pathogens like (), may be contributing to this decline. Our goal was to use a novel dermal swabbing method to measure glucocorticoid (GC) hormones and investigate the relationship among disease and environmental conditions in the critically endangered Wyoming toad (). Our objectives were to (i) validate the use of dermal swabs to measure GCs using an adrenocorticotropic hormone (ACTH) challenge on eight captive toads (4 ACTH: 2 M, 2F and 4 saline as a control: 2 M, 2F), (ii) investigate stress physiology and disease status of toads across six reintroduction sites and (iii) compare dermal cortisol between reintroduced and captive toads.

The Population Genetics, Virulence, and Public Health Concerns of Collected From Rats Within an Urban Environment.

The co-existence of rats and humans in urban environments has long been a cause for concern regarding human health because of the potential for rats to harbor and transmit disease-causing pathogens. Here, we analyze whole-genome sequence (WGS) data from 41 isolates collected from rat feces from 12 locations within the city of Chicago, IL, United States to determine the potential for rats to serve as a reservoir for pathogenic and describe its population structure. We identified 25 different serotypes, none of which were isolated from strains containing significant virulence markers indicating the presence of Shiga toxin-producing and other disease-causing .

Determining the effectiveness of using dermal swabs to evaluate the stress physiology of laboratory cephalopods: A preliminary investigation.

To better comprehend the physiology of cephalopods, we used a minimal invasive technique of skin mucus swabs to measure immunoreactive corticosteroids in three cephalopod species commonly kept in captivity and promoted as new model organisms: Euprymna berryi, Sepia bandensis, and Octopus chierchiae. We compared results between sexes and age classes and then evaluated their stress responses during acclimation to a new habitat. To better understand glucocorticoid production, we conducted an adrenocorticotropic hormone, using Cosyntropin (an adrenocorticotropin (ACTH) analogue) challenge with a saline control and swabbed their mantles at 15-minute intervals for 2 h.

Free-Living Aquatic Turtles as Sentinels of spp. for Water Bodies.

Reptile-associated human salmonellosis cases have increased recently in the United States. It is not uncommon to find healthy chelonians shedding . The rate and frequency of bacterial shedding are not fully understood, and most studies have focused on captive vs.

Group structure, but not dominance rank, predicts fecal androgen metabolite concentrations of wild male mountain gorillas (Gorilla beringei beringei).

Androgens are important mediators of male-male competition in many primate species. Male gorillas' morphology is consistent with a reproductive strategy that relies heavily on androgen-dependent traits (e.g.

The Gombe Ecosystem Health Project: 16 years of program evolution and lessons learned.

Infectious disease outbreaks pose a significant threat to the conservation of chimpanzees (Pan troglodytes) and all threatened nonhuman primates. Characterizing and mitigating these threats to support the sustainability and welfare of wild populations is of the highest priority. In an attempt to understand and mitigate the risk of disease for the chimpanzees of Gombe National Park, Tanzania, we initiated a long-term health-monitoring program in 2004.

Validating the use of fecal glucocorticoid metabolite analysis to assess the adrenocortical activity of the zoo-housed Sichuan takin (Budorcas taxicolor tibetana).

With limited information known about the zoo-housed Sichuan takin (Budorcas taxicolor tibetana), there is a need to gain more knowledge about their basic physiology to be able to better assess their well-being. Our goal was to develop noninvasive methods to evaluate stress physiology in the Sichuan takins housed under human care. The objectives were: (1) validate the use of fecal glucocorticoid metabolite (FGM) analysis to monitor adrenocortical activity and (2) determine the relationship between FGM concentrations and changes in various factors including environmental conditions, reproductive hormones, and social factors.

Factors affecting reproductive traits in male snow leopards ().

Abstract: The population of snow leopards () maintained in US zoos is no longer sustainable due to poor reproductive success. Our objective was to assess reproductive traits in male snow leopards and identify factors (markers of oxidative stress in seminal fluid, surveys of husbandry practices, gonadal and adrenocortical activity, dietary intake of various nutrients, and genetics) that may affect ejaculate traits and subsequent fertility. Ejaculates (2.

Social and ecological factors alter stress physiology of Virunga mountain gorillas ().

Living in a rapidly changing environment can alter stress physiology at the population level, with negative impacts on health, reproductive rates, and mortality that may ultimately result in species decline. Small, isolated animal populations where genetic diversity is low are at particular risks, such as endangered Virunga mountain gorillas (). Along with climate change-associated environmental shifts that are affecting the entire population, subpopulations of the Virunga gorillas have recently experienced extreme changes in their social environment.

Investigation of the utility of feces and hair as non-invasive measures of glucocorticoids in wild black-tailed prairie dogs (Cynomys ludovicianus).

Non-invasive measures of glucocorticoid (GC) hormones and their metabolites, particularly in feces and hair, are gaining popularity as wildlife management tools, but species-specific validations of these tools remain rare. We report the results of a validation on black-tailed prairie dogs (Cynomys ludovicianus), a highly social engineer of the grasslands ecosystem that has experienced recent population declines. We captured adult female prairie dogs and brought them into temporary captivity to conduct an adrenocorticotropic hormone (ACTH) stimulation test, assessing the relationship between plasma GC and fecal glucocorticoid metabolite (FGM) levels following a single injection of a low (4 IU/kg) or high dose (12 IU/kg) of ACTH, compared to a single injection of saline.

Caring for infants is associated with increased reproductive success for male mountain gorillas.

Socioecological theory predicts that male parenting among mammals should be rare due to the large payoffs of prioritizing mating effort over parenting. Although these predictions are generally met, in some promiscuous primate species males overcome this by identifying their offspring, and providing benefits such as protection and resource access. Mountain gorillas, which often organize into multi-male groups, are an intriguing exception.

With a little help from her friends (and family) part II: Non-maternal caregiving behavior and physiology in mammals.

The diversity of competing frameworks for explaining the evolution of non-maternal care in mammals (Part I, this issue) reflects the vast range of behaviors and associated outcomes these theories attempt to subsume. Caretaking comprises a wide variety of behavioral domains, and is mediated by an equally large range of physiological systems. In Part II, we provide an overview of how non-maternal care in mammals is expressed, the ways in which it is regulated, and the many effects such care has on both recipients and caretakers.

With a little help from her friends (and family) part I: the ecology and evolution of non-maternal care in mammals.

In the class Mammalia, most young are cared for exclusively by their mothers. In species where mothers receive help, however, non-maternal caregivers may play a crucial role in development and life history trajectories. In turn, recipients of such care may have important impacts on caregivers of all types.

Atypical experiences of captive chimpanzees () are associated with higher hair cortisol concentrations as adults.

Experiences during early development are influential on the lives of human and non-human primates into adulthood. The population of captive chimpanzees in the USA can provide insight into this relationship, as collectively they have experienced a wide range of exposure to both conspecifics (those raised in natal groups) and humans (those raised as personal pets or performers). Our study investigated chimpanzee exposure to humans using a continuous measure of categorization, the chimpanzee-human interaction index, and the relationship between this experience and cortisol concentrations in adulthood.

Observations of severe and lethal coalitionary attacks in wild mountain gorillas.

In humans and chimpanzees, most intraspecific killing occurs during coalitionary intergroup conflict. In the closely related genus Gorilla, such behavior has not been described. We report three cases of multi-male, multi-female wild mountain gorilla (G.

Maternal effects on offspring stress physiology in wild chimpanzees.

Early life experiences are known to influence hypothalamic-pituitary-adrenal (HPA) axis development, which can impact health outcomes through the individual's ability to mount appropriate physiological reactions to stressors. In primates, these early experiences are most often mediated through the mother and can include the physiological environment experienced during gestation. Here, we investigate stress physiology of dependent offspring in wild chimpanzees for the first time and examine whether differences in maternal stress physiology are related to differences in offspring stress physiology.

The black-footed ferret: on the brink of recovery?

In an attempt to save the species from extinction, the last remaining 18 black-footed ferrets (Mustela nigripes) were trapped up from the wild to initiate a captive breeding program. Nearly 30 years later more than 8,000 black-footed ferrets have been produced in captivity and approximately 4,100 animals have been reintroduced into 20 sites in eight US states (Arizona, New Mexico, Utah, Colorado, Kansas, Wyoming, South Dakota and Montana), Mexico and Canada. However, full recovery of the species has yet to be achieved, mainly due to limited viable habitat, disease and reduced fecundity.

Fecal endocrine monitoring of reproduction in female snow leopards (Uncia uncia).

Although the snow leopard (Uncia uncia) is a common endangered felid species in zoos, little is known about the complex endocrine interactions controlling ovarian function and conception in this species. The goal of this work was to characterize ovarian activity throughout the estrous cycle, nonpregnant luteal phase (pseudopregnancy), and gestation in female snow leopards. This goal was accomplished using an enzyme immunoassay to measure fecal concentrations of estrogen metabolites (E) and progesterone metabolites (P).

Using odor cues to elicit a behavioral and hormonal response in zoo-housed African wild dogs.

Olfactory enrichment, like odor cues, can positively affect behavior, reproductive success, and stress physiology in zoo-housed species. Our goal was to determine if odor cues were enriching to the African wild dog (AWD; Lycaon pictus), a species with a complex social structure and a highly developed sense of smell. Our objectives were to: (1) examine changes in activity levels and stress hormone physiology in response to fecal odor cues from natural competitor and natural/unnatural prey species; and (2) determine whether these odor cues could function as effective enrichment for zoo-housed AWDs.

Disease risk analysis: a tool for primate conservation planning and decision making.

Concern about emerging and re-emerging diseases plays an increasing role in conservation and management of both captive and free-ranging nonhuman primates (NHPs). Managers and policy makers must formulate conservation plans in an arena plagued by uncertainty, complexity, emotion, and politics. The risk analysis paradigm provides a framework that brings together scientists and policy experts to make better decisions for both people and animals.