5-Methylcytosine-modified circRNA-CCNL2 regulates vascular remdeling in hypoxic pulmonary hypertension through binding to FXR2.

Pulmonary hypertension (PH) is a malignant cardiovascular disease with a complex etiology. 5-Methylcytosine (m5C) is a post-transcriptional RNA modification identified in both stable and highly abundant RNAs, with a lower frequency of occurrence in circular RNAs (circRNAs). Nevertheless, the function of m5C-modified circRNAs in the pathogenesis of PH remains uncertain.

Comparative genomics analysis of the reason for C heavy-ion irradiation in improving FeO nanoparticle yield of Acidithiobacillus ferrooxidans.

The FeO nanoparticle synthesized by Acidithiobacillus ferrooxidans have a broad practical value, while the low yield limits their commercial application. Herein, we employed a C heavy-ion beam to induce mutagenesis of A. ferrooxidans BYM and successfully screened a mutant BYMT-200 with a 1.

Integrating Metabolomics and Transcriptomics to Analyse and Reveal the Regulatory Mechanisms of Mung Bean Polyphenols on Intestinal Cell Damage Under Different Heat Stress Temperatures.

Background/objectives: Polyphenols represent a new strategy of dietary intervention for heat stress regulation.

Methods: The metabolic and genetic effects of three heat stress-regulated mung bean polyphenols on mouse small intestinal epithelial Mode-k cells were investigated by metabolomics-transcriptomics correlation analysis at different heat stress levels.

Results: Lipid metabolism, energy metabolism, and nervous system pathways were the key metabolic regulatory pathways.

Discriminating Mung Bean Origins Using Pattern Recognition Methods: A Comparative Study of Raman and NIR Spectroscopy.

The feasibility of the two methodologies was confirmed to compare the results of determining mung bean origins using Raman and Near-Infrared (NIR) spectroscopy. Spectra from mung beans collected in Baicheng City, Jilin Province; Dorbod Mongol Autonomous, Tailai County, Heilongjiang Province; and Sishui County, Shandong Province, China, were analyzed. We established a traceability model using Principal Component Analysis combined with the K-nearest neighbor method to compare the efficacy of these methods in discriminating the origins of the mung beans.

The major roles of intestinal microbiota and TRAF6/NF-κB signaling pathway in acute intestinal inflammation in mice, and the improvement effect by Hippophae rhamnoides polysaccharide.

Acute enteritis, an intestinal disease with intestinal inflammation and injury as the main pathological manifestations. Inhibiting the inflammatory response is critical to the treatment of acute enteritis. Previous studies have shown that the Hippophae rhamnoides polysaccharide (HRP) has strong immune-enhancing effects.

Effectiveness of Differentiating Mold Levels in Soybeans with Electronic Nose Detection Technology.

This study employed electronic nose technology to assess the mold levels in soybeans, conducting analyses on artificially inoculated soybeans with five strains of fungi and distinguishing them from naturally moldy soybeans. Principal component analysis (PCA) and linear discriminant analysis (LDA) were used to evaluate inoculated and naturally moldy samples. The results revealed that the most influential sensor was W2W, which is sensitive to organic sulfur compounds, followed by W1W (primarily responsive to inorganic sulfur compounds), W5S (sensitive to small molecular nitrogen oxides), W1S (responsive to short-chain alkanes such as methane), and W2S (sensitive to alcohols, ethers, aldehydes, and ketones).

Characterization of the Mitochondrial Genome of (Astacidea: Cambaridae) and Its Phylogenetic Implications.

Background: is an endangered freshwater crayfish in China, belonging to the genus Cambaroides, that can act as a complementary host for paragonimus. The objective of this study was to examine the complete mitochondrial genome characteristics and their evolutionary relationships within the Astacidea.

Methods: The analysis of gene rearrangements and evolutionary relationships was conducted through the sequencing of the mitochondrial genome of .

The impact of dietary metabolizable energy levels on the performance of medium-sized geese: A systematic review.

This study aimed to investigate the effects of dietary metabolizable energy levels on various performance indicators of medium-sized geese, with the goal of predicting the optimal range of these energy levels. A comprehensive literature search was conducted across several databases, including Chinese National Knowledge Infrastructure, Wanfang, China Science and Technology Journal Database, PubMed, Web of Science, and ScienceDirect, covering the period from January 1, 2000, to July 1, 2024. The gathered studies focused on the impact of dietary metabolizable energy levels on the production performance of medium-sized geese.

Chromosome-level genome assembly of Monolepta hieroglyphica, two-spotted leaf beetle (Coleoptera: Chrysomelidae).

Monolepta hieroglyphica, in view of its wide-ranging host and highly polyphagous characteristics, has become an important agricultural pest in East and Southeast Asian countries. To better understand its biology and develop control strategies, we present a high-quality chromosome-level genome assembly of M. hieroglyphica, with contig N50 of 18.

MTFAP: a comprehensive platform for predicting and analyzing master transcription factors.

Master transcription factors (MTFs) activate gene expression in pluripotent embryonic stem cells (ESCs) by binding to enhancers and super-enhancers, which precisely control ESC fate. Compelling evidence reveals a strong correlation between the operation of MTFs and the initiation and progression of cancer. Nevertheless, the challenge of identifying MTFs imposes a barrier for researchers.

Luteolin inhibits BHV-1 replication and alleviates virus-induced inflammatory responses by regulating PI3K/AKT pathway.

Bovine herpesvirus type 1 (BHV-1) seriously affects the production safety of the cattle industry and leads to epidemics worldwide. Luteolin (Lut), a flavonoid substance, can be found in vegetables, fruits, and herbs and possesses various biological properties. Here, we found that Lut can dose-dependently and significantly inhibit the cytopathic effects of BHV-1, decrease the viral titer, and suppress the BHV-1 gB gene and VP8 protein levels on bovine nasal turbinate osteoblasts (BT) and bovine kidney epithelial cells (MDBK).

CICADA: a circRNA effort toward the ghost proteome.

Recent studies have confirmed that certain circRNAs encode proteins that are integral to various biological functions. In this study, we present CICADA, an algorithm specifically designed to assess the protein-coding potential and coding products of circRNAs at high throughput, which enables the identification of previously unknown circRNA-encoded proteins. By harnessing the potential of this algorithm, we identified a variety of functional, protein-coding circRNAs in esophageal squamous cell carcinoma and established circRNA translation profiles for diverse types of cancer.

Intrinsic capacity and its association with predictors among Chinese empty nest older adults in communities: a latent class analysis.

Objectives: This study aimed to examine Intrinsic Capacity (IC) subgroups and the association of IC subgroups with IC predictors in Chinese urban empty nesters.

Methods: A convenient sample of 385 older adults aged 60 and above in Community Health Service Center was recruited from Hei Longjiang Province, China, between June 2023 and December 2023. Latent class Analysis (LCA) was conducted to explore IC subgroups using the sensory, cognition, locomotion, psychological, and vitality domains of IC as input variables.

Adsorption properties and competitive adsorption mechanism exhibited by carbon-nanotube-modified biochar for removal of crude oil and Ni(II) pollutants from water.

Carbon-nanotube-modified biochar (CNT3-CBC) with a nanostructured surface was prepared by using cattle manure as the raw material via the impregnation method. This modified biochar was then used to adsorb petroleum and Ni(II) from aqueous solutions. Various physicochemical characterization techniques were employed, including SEM, BET analysis, FTIR, and XPS.

Therapeutic Efficacy of a Synthetic Brain-Targeted HS Donor Cross-Linked Nanomicelle in Autism Spectrum Disorder Rats through Aerobic Glycolysis.

Autism spectrum disorder (ASD) is characterized by cognitive inflexibility and social deficits, with a notably limited range of brain-targeted medications, particularly in the field of nanomedicine. Herein, we introduce the brain-targeted HS donor cross-linked nanomicelle, named mannose-PEG600-lipoic acid (Man-LA). Man-LA demonstrates enhanced stability and precise brain delivery by interacting with glucose transporter 1 (GLUT1) in astrocytes, facilitating a gradual release of HS that is modulated by glutathione (GSH).

, a Small Family of ABA Up-Regulated EAR Motif-Containing Proteins Regulate ABA Responses in Arabidopsis.

The abscisic acid (ABA) signaling pathway is crucial for regulating downstream ABA-responsive genes, which influence plant responses to ABA and abiotic stresses. However, many ABA-responsive genes remain poorly characterized. This study reports on the identification and characterization of ABA up-regulated EAR motif-containing proteins (), a novel family of EAR motif-containing proteins in .

Negative regulation of SREBP-1/FAS signaling molecules activates the RIG-1/TBK1-mediated IFN-I pathway to inhibit BVDV replication.

For many viruses, controlling the process of infection is largely dependent on the enzymes of the fatty acid synthesis (FAS) pathway. An appealing therapeutic target in antiviral research is fatty acid synthetase (FASN), a crucial enzyme in the FAS pathway. Bovine viral diarrhea, caused by the Bovine viral diarrhea virus (BVDV), is a significant viral infectious disease posing a substantial threat to global animal husbandry.

Metabolomics of black beans ( L.) during atmospheric pressure steaming and in vitro simulated digestion.

In the paper, metabolomics techniques based on UHPLC-QE-MS were used to study raw black beans, steaming black beans, and their in vitro digestion products. The results show that the three groups of raw black beans, atmospheric pressure-steamed black beans, and their in vitro digests comprised 922, 945, and 878 characteristic metabolites, respectively, dominated by amino acids, organic acids, polyphenols, and sugars. After screening the differential metabolites, content comparison, the content of amino acids, sugars, and phenolics in black beans was found to be increased after atmospheric steaming.

Effect of γ-irradiation combined with enzymatic modification on the physicochemical properties of defatted rice bran dietary fiber.

This study comprehensively examines how combining γ-irradiation and enzymatic modification influences the microstructure and physicochemical properties of dietary fiber (DF) obtained from defatted rice bran. The resulting yields of soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were measured at 13.38 ± 0.