Molecular identification and genetic-polymorphism analysis of Fasciola flukes in Dali Prefecture, Yunnan Province, China.

This study aimed to identify species of Fasciola flukes in Dali Prefecture (Yunnan Province, China) and analyze their genetic diversity. Fasciola flukes (n = 122) were collected from cattle livers in a farmers' market in Xiaguan Town, Dali Prefecture. Nucleotide sequences of ribosomal internal transcribed spacer (ITS) as well as nicotinamide adenine dinucleotide dehydrogenase subunit 1 (ND1) and mitochondrial cytochrome c oxidase subunit 1 (CO1) were amplified, sequenced, and subjected to homology analysis.

[Assessment of schistosomiasis transmission risk in Nanjian County of Yunnan Province in 2019].

Objective: To assess the schistosomiasis transmission risk in Nanjing County, so as to provide the scientific evidence for the development of the schistosomiasis control strategy and consolidation of the control achievements.

Methods: On May 2019, the Deqiu Village in Deqiu Town and Baiyun Village in Nanjing Town of Yunnan Province, where schistosomiasis were historically relatively highly endemic, were selected to assess the risk of transmission of schistosomiasis by means of a retrospective review of the data pertaining to the historical endemic situation and schistosomiasis control, combined with a cross-sectional survey of snail and wild feces distribution.

Results: During the period between 2017 and 2018, the mean sero-prevalence rates of infections were 6.

[Molluscicidal activity of 25% wettable powder of pyriclobenzuron sulphate against ].

Objective: To evaluate the molluscicidal activity of a novel molluscicide pyriclobenzuron against in the mountain regions of Yunan Province, and test its toxicity to fish, so as to provide scientific evidence for the extensive application of this molluscicide in schistosomiasis-endemic foci of Yunan Province.

Methods: In the laboratory and snail-breeding field of Dali Prefecture, Yunnan Province, the molluscicidal activity of 5% wettable powder of pyriclobenzuron sulphate (25% PBU) against was assessed by using the immersion and spraying method, and the acute toxicity of 25% PBU to carp fries was tested, while 25% wettable powder of niclosamide ethanolamine salt (50% WPNES) served as a control.

Results: The 1-, 2- and 3-day 25% PBU LC50 and LC90 values were 0.

[Effect of comprehensive control and management of schistosomiasis at plateaus regions in Yunnan Province].

Objective: To evaluate the effect of comprehensive control and management of schistosomiasis at plateaus regions in Yunnan Province.

Methods: Xiaolian and Kangfu villages at plateaus regions where schistosomiasis was endemic were selected as study areas from 2006 to 2011, the schistosomiasis comprehensive control measures were conducted, and these measures included the health education, chemotherapy, stool and water management, grazing forbidden, Oncomelania snail control, etc. combined with the infrastructure of farmland water conservancy, agricultural comprehensive development, adjustment of industry structure, returning farmland to forest, ditch hardening, and aquaculture.

[Effect of snail control of niclosamide by soil mixing and spraying methods in high dam of terrace].

The snail control efffect of niclosamide by soil mixing and spraying method were compared, and the results showed that the effects of the two methods were similar. Though there was difficulties in getting soil and mixing durg with soil, and the cost was high, the effect of soil mixing method in some special environment such as stone ditches and terraces.

[Investigation on outbreak of angiostrongyliasis cantonensis due to consumption of snail food in Dali City].

Objective: To investigate the potential cause of an outbreak of eosinophilic meningitis in Dali City in March, 2011.

Methods: A retrospective investigation was performed among 18 consumers who had meal together. The clinical documents of inpatients were analyzed and the diagnoses were checked.

[Studies on man-cattle-man infection cycle of Sarcocystis hominis in Yunnan].

The present paper reports on the results of an experimental study on man-cattle-man infection of Sarcocystis hominis, found in Yunnan Province. About ten thousand sporocysts collected from the feces of persons naturally infected with Sarcocystis hominis were fed to a calf, which was dissected 150 days later. Numerous cysts of Sarcocystis hominis were found in the cardiac and skeletal muscles.