Case Report: Hybrid closed-loop insulin pump can significantly improve awareness of hypoglycemia.

Impaired awareness of hypoglycemia remains an issue even in the era of modern technologies, as patients with type 1 diabetes (T1DM) face stricter requirements for glycemic targets. The evaluation of hypoglycemia awareness can be accomplished using questionnaires (Clarke and Gold scores) in combination with clinical appearance and sensor data. A 45-year-old man with T1DM was referred to our clinic in July 2019 due to impaired hypoglycemia awareness and repeated severe hypoglycemic episodes resulting in unconsciousness.

Oligomerization Function of the Native Exon 5 Sequence of Ameloblastin Fused with Calmodulin.

The evolution of proteins is primarily driven by the combinatorial assembly of a limited set of pre-existing modules known as protein domains. This modular architecture not only supports the diversity of natural proteins but also provides a robust strategy for protein engineering, enabling the design of artificial proteins with enhanced or novel functions for various industrial applications. Among these functions, oligomerization plays a crucial role in enhancing protein activity, such as by increasing the binding capacity of antibodies.

Highly Stable, Flexible, Anticorrosive Coating of Metalized Nonwoven Textiles for Durable EMI Shielding and Thermal Properties.

In the present research, an advanced silane-bonded polydopamine (PDA) coating through a simple, low-cost, and highly effective technique was employed to enhance the stability of copper-coated electromagnetic shielding fabrics. Coating the metalized nonwoven PET fabric with PDA can protect it from oxidation, mechanical forces, and extreme chemical conditions such as acid and alkali corrosion. The coated nonwoven fabric retained its excellent electromagnetic shielding effect even after machine- and handwashing cycles, showing average shielding effectiveness (SE) values above 41 dB for PDA@MEFTEX and Si-QAC/PDA@MEFTEX samples, and the average SE remained consistently above 39 dB under acidic and alkaline conditions.

From Foreshock 30-Second Waves to Magnetospheric Pc3 Waves.

Ultra-low frequency waves, with periods between 1-1000 s, are ubiquitous in the near-Earth plasma environment and play an important role in magnetospheric dynamics and in the transfer of electromagnetic energy from the solar wind to the magnetosphere. A class of those waves, often referred to as Pc3 waves when they are recorded from the ground, with periods between 10 and 45 s, are routinely observed in the dayside magnetosphere. They originate from the ion foreshock, a region of geospace extending upstream of the quasi-parallel portion of Earth's bow shock.

Selenium nanoparticles: influence of reducing agents on particle stability and antibacterial activity at biogenic concentrations.

Selenium nanoparticles (SeNPs) have recently attracted attention for their antimicrobial and anticancer activities. Nevertheless, their use remains limited due to stability issues. The objective of this study is to investigate the impact of different reaction conditions (including the reducing and stabilizing agents, as well as reaction temperature) on the water dispersion characteristics, stability, and biological activity of SeNPs.

Validating the Genus Pocheina (Acrasidae, Heterolobosea, Discoba) Leads to the Recognition of Three Major Lineages Within Acrasidae.

Pocheina and Acrasis are two genera of heterolobosean sorocarpic amoebae within Acrasidae that have historically been considered close relatives. The two genera were differentiated based on their differing fruiting body morphologies. The validity of this taxonomic distinction was challenged when a SSU rRNA phylogenetic study placed an isolate morphologically identified as "Pocheina" rosea within a clade of Acrasis rosea isolates.

Bacterial genera in the fluids from apical periodontitis-related radicular cysts: An observational study.

Aim: This study aimed to evaluate bacteriome profiles (diversity, composition and relative abundances of bacterial genera) of the fluids from apical periodontitis (AP)-related radicular cysts (RCs).

Methodology: This observational study included 29 patients with AP and RC with complete sample triplets (supragingival plaque, cryopulverized tooth and cystic fluid). The bacteriome profiles of each matrix as well as of negative controls (NCs) were examined using 16S rRNA amplicon sequencing.

Altitudinal impacts on phytochemical composition and mycorrhizal diversity of Reinwardtia indica Dumort, a medicinally valuable herb.

Background: Reinwardtia indica, a highly valued ethnomedicinal plant, has been traditionally used to treat various ailments due to its rich phytochemical composition. However, the impact of environmental factors, particularly altitude, on its medicinal properties remains unexplored. This study investigates the effects of altitudinal variation on phytochemicals, mycorrhizal diversity, and soil physico-chemical parameters of R.

Use of the vessel sealer in paraaortic lymphadenectomy in the robotic assisted approach in endometrial cancer.

Minimally invasive surgery is the method of choice in endometrial cancer. Experience in procedures assisted by a robotic system is growing rapidly. One of the new bipolar ones is a Vessel Sealer, with sealing and cutting function.

Effectiveness of the XBB.1.5 COVID-19 Vaccines Against SARS-CoV-2 Hospitalisation Among Adults Aged ≥ 65 Years During the BA.2.86/JN.1 Predominant Period, VEBIS Hospital Study, Europe, November 2023 to May 2024.

We estimated the effectiveness of the adapted monovalent XBB.1.5 COVID-19 vaccines against PCR-confirmed SARS-CoV-2 hospitalisation during the BA.

The synthesis and influence of the novel bispyridinium compound LB1 on the effectiveness of the standard antidotal treatment of organophosphorus nerve agent intoxicated mice and some structure-activity considerations.

The design of MB327, a bispyridinium compound that ameliorates the nicotinic effects of acute organophosphorus nerve agent (NA) intoxication, followed an observation made by the German pharmacologist Klaus Schoene in the 1970s, who noted therapeutic activity in bispyridinium molecules missing the usual oxime group, CH=NOH. Some of these compounds protected mice against soman. One structurally related to obidoxime called HY10 had this action.

Evolution of circadian clock and light-input pathway genes in Hemiptera.

Circadian clocks are timekeeping mechanisms that help organisms anticipate periodic alterations of day and night. These clocks are widespread, and in the case of animals, they rely on genetically related components. At the molecular level, the animal circadian clock consists of several interconnected transcription-translation feedback loops.

Effect of combined and intensive rehabilitation on cognitive function in patients with Alzheimer's disease evaluated through a randomized controlled trial.

This study investigates the impact of combined special education and occupational therapy intervention on cognitive functions in Alzheimer's patients. Specifically, it evaluates changes measured by the Addenbrooke's Cognitive Examination (ACE-R) after six months compared to a control group receiving standard care. A longitudinal, controlled experiment was conducted with random assignment to experimental and control groups.

Reduced removal of waste products from energy metabolism takes center stage in human brain aging.

Despite extensive research on neuroimaging correlates of human brain aging, there is little mechanistic insight into how they are linked to loss of brain function. Previous studies on the role of cerebral blood flow (CBF) in supporting brain function have focused on delivery of nutrients, namely oxygen and glucose. However, CBF is required also to clear the byproducts of energy metabolism, namely CO and protons.

Zwitterionic DNA: enzymatic synthesis of hypermodified DNA bearing four different cationic substituents at all four nucleobases.

We designed and synthesized a set of four 2'-deoxyribonucleoside 5'-O-triphosphates (dNTPs) bearing cationic substituents (protonated amino, methylamino, dimethylamino and trimethylammonium groups) attached to position 5 of pyrimidines or position 7 of 7-deazapurines through hex-1-ynyl or propargyl linker. These cationic dNTPs were studied as substrates in enzymatic synthesis of modified and hypermodified DNA using KOD XL DNA polymerase. In primer extension (PEX), we successfully obtained DNA containing one, two, three, or (all) four modified nucleotides, each bearing a different cationic modification.

Environment-like exposure of free-living stages of Haemonchus contortus to albendazole: The intra- and intergeneration stability of altered expression of UDP-glycosyl transferases and ABC-transporters.

Anthelmintic drugs are widespread environmental contaminants, but their impact is still poorly understood. Although contact of parasitic nematode Haemonchus contortus with traces of anthelmintic drug albendazole (ABZ) altered the expression and activity of several UDP-glycosyl transferases (UGTs) and P-glycoproteins (Pgps, belonging to ABC-transporters), key enzymes in endogenous and xenobiotic metabolism, it is not known whether these changes will last during the life cycle and pass to the next generations. In the present study simulating the environmental-like exposure, free-living stages of H.

The impact of noise on green open space value.

I investigate the effect of noise on the amenity value of urban green open spaces in Prague, Czech Republic. First, I use standard hedonic pricing model exploiting cross-sectional and quasi-experimental variation in the apartment price data and then I analyse green open spaces quality inferred from a quantitative spatial model. Results show that increasing size of quiet green open spaces (with noise below 60 dB) by 10% increases local apartment prices by 0.

Bioaccumulation of PCBs and OCPs in Antarctic phytoplankton and zooplankton: Insights into bioconcentration and biomagnification in Fildes Bay.

This study was conducted at Fildes Bay, King George Island, Antarctica, from November 30th, 2019, to January 30th, 2020. We determined the concentrations of Organochlorine Pesticides (OCPs) and Polychlorinated Biphenyls (PCBs) in phytoplankton, and zooplankton. The most abundant compounds found were hexachlorobenzene (HCB) in phytoplankton and γ-HCH in zooplankton.

Rectocervical Fistula Repair After Laparoscopic Supracervical Hysterectomy and Laparoscopic Sacrocervicopexy.

Introduction And Hypothesis: Rectocervical fistula after a urogynecological surgery has never been described. In this video article, we present occurrence of this complication after laparoscopic supracervical hysterectomy and laparoscopic sacrocervicopexy (LSCH + LSC) and how it was treated.

Methods: This was a case of a 47-year-old patient who had a laparoscopic supracervical hysterectomy and laparoscopic sacrocervicopexy (LSCH + LSC) for stage two pelvic organ prolapse.

Contexts, affective and physical states and their variations during physical activity in older adults: an intensive longitudinal study with sensor-triggered event-based ecological momentary assessments.

Background: To design effective tailored interventions to promote physical activity (PA) among older adults, insights are needed into the contexts in which older adults engage in PA and their affective and physical experiences. Sensor-triggered event-based ecological momentary assessment (EMA) is an innovative method for capturing real-life contexts, as well as affective and physical states, during or immediately after specific events, such as PA. This study aimed to (1) describe the physical and social contexts, and the affective and physical states during PA among older adults, (2) evaluate how these constructs fluctuate during PA episodes, and (3) describe affective states during PA according to the context.

Methods and Models in Mammary Gland Biology and Breast Cancer Research.

The collection on Methods and Models in Mammary Gland Biology and Breast Cancer Research showcases recent advances in tools and models that enhance our understanding of mammary gland development and breast cancer. This collection includes sixteen articles, collectively addressing approaches to investigate key aspects of mammary gland biology and tumorigenesis, including hormonal signaling, tissue architecture, tumor microenvironment, and species-specific mammary development. The issue highlights innovations such as optimized progesterone receptor reporters, improved menopause models, and 3D-printed mammary epithelial structures.

DIAGNOSTIC EVALUATION AND CLINICAL FINDINGS IN CHILDREN WITH PERSISTENT TACHYPNEA OF INFANCY/NEUROENDOCRINE CELL HYPERPLASIA OF INFANCY: A EUROPEAN MULTICENTER RETROSPECTIVE STUDY.

Background: Persistent tachypnea of infancy (PTI)/neuroendocrine cell hyperplasia of infancy (NEHI) is a form of childhood interstitial lung disease (chILD) that predominantly affects young children. Although it is one of the most common chILDs, there is no unified diagnostic approach specific to this condition.

Research Question: Is there a difference in the clinical presentation and the diagnostic approach in PTI/NEHI patients between the European countries?

Study Design And Methods: This was a European multicenter, retrospective, observational study.

Occurrence of Current-Use Pesticides in Sediment Cores from Lakes and Peatlands in Pristine Mountain Areas of Brazilian National Parks.

This study assessed the occurrence of current-use pesticides in sediment cores from six lakes and peatlands in high-altitude (1,952-2,374 meters) pristine areas within two southeastern Brazilian National Parks: Itatiaia National Park (IT-NP) and Serra dos Órgãos National Park (SO-NP). We sampled three sediment cores from lakes at IT-NP and three from peatlands at SO-NP, totaling 60 subsamples. Among the 38 current-use pesticides assessed, 17 were found in at least one sample, with 14 - including herbicides, insecticides, fungicides, and acaricides - identified in both parks.

Benefits of Glucagon-like Peptide-1 Receptor Agonists after Kidney Transplantation.

Objectives: Benefits of glucagon-like peptide-1 receptor agonists (GLP-1 RA) in kidney transplant recipients have not been adequately studied.

Methods: We retrospectively examined the effects of GLP-1RA on mortality, kidney outcomes and metabolic parameters in kidney transplant (KT) recipients with T2DM treated versus not treated with GLP-1 RA. A reference group of KT recipients not treated with GLP-1RA was used for comparison.