Design, synthesis, and activity evaluation of C-8 arylated luteolin derivatives as influenza endonuclease inhibitors.

The polymerase acidic (PA) subunit of the influenza virus, an endonuclease of the RNA-dependent RNA polymerase, represents a viable target for anti-influenza therapies, as evidenced by the efficacy of the FDA-approved drug Xofluza. A characteristic feature of endonuclease inhibitors is their ability to chelate Mg or Mn ions within the enzyme's catalytic site. Previously, our studies identified luteolin and its C-8-glucoside orientin as potent endonuclease inhibitors.

Comparable outcomes in single versus multiple septal branches alcohol ablation for obstructive hypertrophic cardiomyopathy.

Background: The optimal number of septal branches to target during initial alcohol septal ablation (ASA) for hypertrophic obstructive cardiomyopathy (HOCM) remains a subject of debate. It is unclear whether to proceed with ASA of additional septal branches if a satisfactory hemodynamic effect has not been achieved following ablation of the first branch.

Methods: Using propensity score matching analysis, we compared patients who achieved satisfactory outcomes after ASA of a single septal branch with those in whom additional branches were ablated.

[Diphtheria - a 21st century zoonosis?].

Besides Corynebacterium diphtheriae, C. ulcerans can cause diphtheria in humans, and its incidence has been increasing alarmingly in recent years. The source and reservoir of these infections are a wide range of domestic and wild animals, in which C.

[Alternative treatment options for multidrug-resistant HIV-1 infection - first use of lenacapavir in the Czech Republic].

In this short communication we describe the phenomenon of multidrug-resistant HIV-1 infection, possible causes and treatment op-tions in these cases. The case report presents the successful use of a drug lenacapavir in a non-adherent patient with HIV infection trea-ted at the HIV center of the Clinic of Infectious Diseases, University Hospital Bulovka, Prague.

Acute respiratory failure in influenza as a cause of out-of-hospital cardiac arrest.

Influenza is an infectious disease caused by influenza A, B, and sometimes C viruses. The disease is preventable in all age groups thanks to annual seasonal vaccination. Population groups at high risk of severe illness with complications include seniors, pregnant women, and young children.

[Role of aztreonam/avibactam in antibiotic therapy].

The article reviews current options for the treatment of infections caused by carbapenemase-producing Enterobacterales, including the role of aztreonam/avibactam.

[Epidemiological situation regarding SARS-CoV-2 in the Havlíčkův Brod region from April 2020 to September 2022].

Objective: In early March 2020, the first cases of the disease caused by SARS-CoV-2 were confirmed in the Czech Republic. From April 2020 to the end of September 2022, more than 80,000 samples were examined in the microbiology laboratory of the Havlíčkův Brod Hospital. The article summarizes data obtained during the COVID-19 pandemic.

Beliefs in Misinformation About COVID-19 and the Russian Invasion of Ukraine Are Linked: Evidence From a Nationally Representative Survey Study.

Background: Detrimental effects of misinformation were observed during the COVID-19 pandemic. Presently, amid Russia's military aggression in Ukraine, another wave of misinformation is spreading on the web and impacting our daily lives, with many citizens and politicians embracing Russian propaganda narratives. Despite the lack of an objective connection between these 2 societal issues, anecdotal observations suggest that supporters of misinformation regarding COVID-19 (BM-C) have also adopted misinformation about the war in Ukraine (BM-U) while sharing similar media use patterns and political attitudes.

The clade in the Holarctic region: existing names evaluated and seven new species described.

is a commonly reported species, characterized by a yellow pileus, yellow colors on the stipe, pleurocystidia provided with digitiform excrescences, and a pileipellis composed of long, fusiform elements. Several species related to have been described from the temperate and boreal areas of the Northern Hemisphere, and previous phylogenetic studies have shown that more than one species can be recognized around , but it was unclear how many, or which names, would be correct for them. We studied 141 holarctic collections in the /leoninus clade and available type collections of species in this group.

Interfacing with the Brain: How Nanotechnology Can Contribute.

Interfacing artificial devices with the human brain is the central goal of neurotechnology. Yet, our imaginations are often limited by currently available paradigms and technologies. Suggestions for brain-machine interfaces have changed over time, along with the available technology.

A monomer-dimer switch modulates the activity of plant adenosine kinase.

Adenosine undergoes ATP-dependent phosphorylation catalyzed by adenosine kinase (ADK). In plants, ADK also phosphorylates cytokinin ribosides, transport forms of the hormone. Here, we investigated the substrate preferences, oligomeric states and structures of ADKs from moss (Physcomitrella patens) and maize (Zea mays) alongside metabolomic and phenotypic analyses.

Pre-industrial Use of Bauxite by Late Gothic Goldsmith Masters: Analytical Evidence and Experimental Study.

The turn of the 15th and the 16th century was marked by turmoil including invasion of the Turks in the Balkans. Consequently, trade routes to the Mediterranean were disrupted, resulting in sudden demand for alternative sources of imported materials. For example, direct import of potassium alum had been gradually replaced by its local production from alunite, alum schists or grey pyritic bauxite.

The HOX code of human adult fibroblasts reflects their ectomesenchymal or mesodermal origin.

Fibroblasts, the most abundant cell type in the human body, play crucial roles in biological processes such as inflammation and cancer progression. They originate from the mesoderm or neural-crest-derived ectomesenchyme. Ectomesenchyme-derived fibroblasts contribute to facial formation and do not express HOX genes during development.

Morphological spore-based characterisation and molecular approaches reveal comparable patterns in glomeromycotan communities.

Traditionally, characterisation and comparison of AMF communities has been carried out by morphological identification of asexual spores in soil. In recent decades, molecular methods such as soil metabarcoding have become more popular than morphological identification of spores, but direct comparisons of the efficiency of both approaches have been rare. In this study, we compared AMF communities in soil samples from vegetable farms using both morphological and molecular methods (internal transcribed spacer, ITS, markers).

High temperature interactions of lead palmitate with linseed oil.

This study investigates the high-temperature interactions between lead palmitate (Pb(C16)) and linseed oil, revealing complex phase transformations and structural changes critical for understanding metal soap formation in oil paintings. Through complementary analytical techniques including DSC, XRPD, FTIR, and solid-state NMR, we identified two significant phenomena: the formation of mixed lead palmitate-stearate compounds and the emergence of a high-temperature lead palmitate polymorph. When heated to 112 °C, lead palmitate in linseed oil undergoes partial conversion to mixed lead palmitate-stearate complexes, likely due to interactions with stearic acid present in the oil either in a free form or as a part of triglycerides.

Anaerobic corrosion of steel wire by under alkaline autotrophic conditions.

Microbially induced corrosion (MIC), caused by iron-cycling microorganisms that directly uptake electrons from metallic iron, is a serious economic and environmental problem. Iron corrosion is inhibited at pH above 9.0 in the presence of carbonate by the formation of a passivating film, but the possibility of direct oxidation of metallic iron by anaerobic alkaliphiles has not been thoroughly investigated.

Phosphorylation at the Helm: Kinase-Mediated Regulation of Primary Cilia Assembly and Disassembly.

The primary cilium serves as an antenna of most vertebrate cells and is important for conveying cues from several signaling pathways into appropriate cellular responses during development and homeostasis. Cilia assembly and disassembly processes are thought to be strictly controlled; however, the precise nature of molecular events underlying this control still awaits full resolution. Through their enzymatic activity, kinases function as flexible yet highly controllable regulators of a vast variety of cellular processes.

Antecedents of perceived teacher work ability: a comprehensive model across work and non-work domains.

Introduction: This study investigates the antecedents of perceived teacher work ability, a critical construct for addressing challenges associated with extending working lives and maintaining sustained professional engagement in the teaching profession. Grounded in the job demands-resources (JD-R) model, this cross-sectional study investigates the relationships between job demands (quantitative, cognitive, and emotional), job resources (supervisor support, coworker support, and autonomy), burnout, and work-life conflict in shaping perceived teacher work ability.

Methods: Data were obtained from 841 Czech primary and lower secondary school teachers (86.

Fine structure in the decay of Hg and Au.

The -decay fine structure of Hg and Au was studied by means of decay spectroscopy. Two experiments were performed at the Accelerator Laboratory of the University of Jyväskylä (JYFL), Finland, utilizing the recoil separator RITU and a digital data acquisition system. The heavy-ion induced fusion-evaporation reactions Kr +  Ru and Kr +  Mo were used to produce the Hg and Au nuclei, respectively.

Optical genome and epigenome mapping of clear cell renal cell carcinoma.

Cancer cells display complex genomic aberrations that include large-scale genetic rearrangements and epigenetic modulation that are not easily captured by short-read sequencing. This study presents a novel approach for simultaneous profiling of long-range genetic and epigenetic changes in matched cancer samples, focusing on clear cell renal cell carcinoma (ccRCC). ccRCC is a common kidney cancer subtype frequently characterized by a 3p deletion and the inactivation of the von Hippel-Lindau () gene.

Case Report: Hybrid closed-loop insulin pump can significantly improve awareness of hypoglycemia.

Impaired awareness of hypoglycemia remains an issue even in the era of modern technologies, as patients with type 1 diabetes (T1DM) face stricter requirements for glycemic targets. The evaluation of hypoglycemia awareness can be accomplished using questionnaires (Clarke and Gold scores) in combination with clinical appearance and sensor data. A 45-year-old man with T1DM was referred to our clinic in July 2019 due to impaired hypoglycemia awareness and repeated severe hypoglycemic episodes resulting in unconsciousness.

Oligomerization Function of the Native Exon 5 Sequence of Ameloblastin Fused with Calmodulin.

The evolution of proteins is primarily driven by the combinatorial assembly of a limited set of pre-existing modules known as protein domains. This modular architecture not only supports the diversity of natural proteins but also provides a robust strategy for protein engineering, enabling the design of artificial proteins with enhanced or novel functions for various industrial applications. Among these functions, oligomerization plays a crucial role in enhancing protein activity, such as by increasing the binding capacity of antibodies.

Highly Stable, Flexible, Anticorrosive Coating of Metalized Nonwoven Textiles for Durable EMI Shielding and Thermal Properties.

In the present research, an advanced silane-bonded polydopamine (PDA) coating through a simple, low-cost, and highly effective technique was employed to enhance the stability of copper-coated electromagnetic shielding fabrics. Coating the metalized nonwoven PET fabric with PDA can protect it from oxidation, mechanical forces, and extreme chemical conditions such as acid and alkali corrosion. The coated nonwoven fabric retained its excellent electromagnetic shielding effect even after machine- and handwashing cycles, showing average shielding effectiveness (SE) values above 41 dB for PDA@MEFTEX and Si-QAC/PDA@MEFTEX samples, and the average SE remained consistently above 39 dB under acidic and alkaline conditions.

From Foreshock 30-Second Waves to Magnetospheric Pc3 Waves.

Ultra-low frequency waves, with periods between 1-1000 s, are ubiquitous in the near-Earth plasma environment and play an important role in magnetospheric dynamics and in the transfer of electromagnetic energy from the solar wind to the magnetosphere. A class of those waves, often referred to as Pc3 waves when they are recorded from the ground, with periods between 10 and 45 s, are routinely observed in the dayside magnetosphere. They originate from the ion foreshock, a region of geospace extending upstream of the quasi-parallel portion of Earth's bow shock.

Selenium nanoparticles: influence of reducing agents on particle stability and antibacterial activity at biogenic concentrations.

Selenium nanoparticles (SeNPs) have recently attracted attention for their antimicrobial and anticancer activities. Nevertheless, their use remains limited due to stability issues. The objective of this study is to investigate the impact of different reaction conditions (including the reducing and stabilizing agents, as well as reaction temperature) on the water dispersion characteristics, stability, and biological activity of SeNPs.