R2DT: A COMPREHENSIVE PLATFORM FOR VISUALISING RNA SECONDARY STRUCTURE.

RNA secondary (2D) structure visualisation is an essential tool for understanding RNA function. R2DT is a software package designed to visualise RNA 2D structures in consistent, recognisable, and reproducible layouts. The latest release, R2DT 2.

Comprehensive cost-of-illness analysis of pressure ulcer treatment: A real-world study at a Czech university hospital.

Pressure ulcers (PUs) impose a significant economic burden on healthcare systems, affecting patient quality of life and leading to substantial treatment costs. This study presents a cost-of-illness analysis of PU treatment in hospitalized patients in the Czech Republic, based on real-world clinical data. The analysis was conducted using a comprehensive methodology at a Czech university hospital, involving 304 hospitalizations.

Disrupting SARS-CoV-2 Spike Protein Activity: A Virtual Screening and Binding Assay Study.

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a respiratory virus that emerged in late 2019 and rapidly spread worldwide, causing the COVID-19 pandemic. The spike glycoprotein (S protein) plays a crucial role in viral target recognition and entry by interacting with angiotensin, converting enzyme 2 (ACE2), the functional receptor for the virus, via its receptor binding domain (RBD). The RBD availability for this interaction can be influenced by external factors, such as fatty acids.

Pyroxsulam Resistance in : An Emerging Challenge in Crop Protection.

, a prevalent weed in Czech winter wheat fields, has developed resistance to ALS-inhibiting herbicides due to their frequent use. This study reports a biotype of resistant to pyroxsulam, with cross and multiple resistance to iodosulfuron, propoxycarbazone, pinoxaden, and chlortoluron. Dose-response experiments revealed high resistance of both R1 and R2 biotypes to pyroxsulam, with resistance factors (RF) of 6.

Bio-concentration of hazardous metals in migrant shorebirds in a key conservation reserve and adjoining areas on the west coast of India.

Heavy metal pollution is a growing environmental concern as it causes the degradation of wetlands by affecting the organisms at different trophic levels. Shorebirds typically feed on benthic invertebrates including polychaete worms, crustaceans and molluscs. Thus, the assessment of bioconcentration of heavy metals in shorebirds provides an insight into the extent of bioaccumulation of these hazardous metals in the upper trophic levels.

Nuclear and mitochondrial genome assemblies for the endangered wood-decaying fungus Somion occarium.

Somion occarium is a wood-decaying bracket fungus belonging to an order known to be rich in useful chemical compounds. Despite its widespread distribution, S. occarium has been assessed as endangered on at least one national Red List, presumably due to loss of old-growth forest habitat.

A collaborative network analysis for the interpretation of transcriptomics data in Huntington's disease.

Rare diseases may affect the quality of life of patients and be life-threatening. Therapeutic opportunities are often limited, in part because of the lack of understanding of the molecular mechanisms underlying these diseases. This can be ascribed to the low prevalence of rare diseases and therefore the lower sample sizes available for research.

Virtual reality-based neuroproprioceptive physiotherapy in multiple sclerosis: a protocol for a double-arm randomised assessor-blinded controlled trial on upper extremity function, postural function and quality of life, with molecular and functional MRI assessment.

Introduction: Upper limb (UL) impairment is common in people with multiple sclerosis (pwMS), and functional recovery of the UL is a key rehabilitation goal. Technology-based approaches, like virtual reality (VR), are increasingly promising. While most VR environments are task-oriented, our clinical approach integrates neuroproprioceptive 'facilitation and inhibition' (NFI) principles.

Tropical forest clearance impacts biodiversity and function, whereas logging changes structure.

The impacts of degradation and deforestation on tropical forests are poorly understood, particularly at landscape scales. We present an extensive ecosystem analysis of the impacts of logging and conversion of tropical forest to oil palm from a large-scale study in Borneo, synthesizing responses from 82 variables categorized into four ecological levels spanning a broad suite of ecosystem properties: (i) structure and environment, (ii) species traits, (iii) biodiversity, and (iv) ecosystem functions. Responses were highly heterogeneous and often complex and nonlinear.

Geochemical insights into plant uptake of Technology-critical elements: A case study on lettuce from European soils.

While vegetable uptake of traditional metal contaminants is a well-studied pathway to human exposure and risk, a paucity of information exists on the uptake of emerging metal contaminants. This study evaluated the uptake of the Technology-critical elements (TCEs) gallium (Ga), germanium (Ge), niobium (Nb), tantalum (Ta), thallium (Tl), and rare earth elements (REEs) into lettuce cultivated in 21 European urban soils. For comparison, the uptake of cadmium (Cd) was also analysed.

Characterization of Hemoglobin Variants by Capillary Electrophoresis, UV-Vis, and FTIR Spectroscopy.

Hemoglobinopathies, hereditary disorders affecting the structure or production of hemoglobin, were detected by routine HbA measurements by capillary electrophoresis (CE) at the University Hospital Motol, Prague. The potential of ultraviolet-visible (UV-Vis) and Fourier-transform infrared (FTIR) spectroscopy for the detection and characterization of hemoglobinopathies was investigated. FTIR spectra were recorded with a very high resolution (0.

Maintenance therapy with a P2Y12 receptor inhibitor after cangrelor in patients with acute coronary syndrome. The ELECTRA-SIRIO 2 investigators' viewpoint.

According to the ESC guidelines, cangrelor may be considered in P2Y12-inhibitor-naïve acute coronary syndrome (ACS) patients undergoing percutaneous coronary intervention (PCI). The aim of this review is to summarize available evidence on the optimal maintenance therapy with P2Y12 receptor inhibitor after cangrelor. Transitioning from cangrelor to a thienopyridine, but not ticagrelor, can be associated with a drug-drug interaction (DDI); therefore, a ticagrelor loading dose (LD) can be given any time before, during, or at the end of a cangrelor infusion, while a LD of clopidogrel or prasugrel should be administered at the time the infusion of cangrelor ends or within 30 minutes before the end of infusion in the case of a LD of prasugrel.

Complex immunohistochemical and molecular study on 5 cases of ovarian juvenile granulosa cell tumors reveals a consistent alteration in the PI3K/AKT/mTOR signaling pathway.

Background: Juvenile granulosa cell tumor (JGCT) of the ovary is a rare tumor with distinct clinicopathological and hormonal features primarily affecting young women and children. We conducted a complex clinicopathological, immunohistochemical, and molecular analysis of five cases of JGCT.

Methods: The immunohistochemical examination was performed with 32 markers, including markers that have not been previously investigated.

Clinical management of female patients with Fabry disease based on expert consensus.

Fabry disease is an X-linked lysosomal storage disorder that causes accumulation of glycosphingolipids in body tissues and fluids, leading to progressive organ damage and life-threatening complications. It can affect both males and females and can be classified into classic or later-onset phenotypes. The disease severity in females ranges from asymptomatic to the more severe, classic phenotype.

Evaluation of Chemical Composition and Anti-Staphylococcal Activity of Essential Oils from Leaves of Two Indigenous Plant Species, and .

Many indigenous plants of the Philippines, including essential oil-bearing species, remain phytochemically and pharmacologically unexplored. In this study, the chemical composition of leaf essential oils (EOs) hydrodistilled from (Lauraceae) and (Piperaceae) was determined using dual-column (HP-5MS/DB-WAX)/dual-detector gas chromatography and mass spectrometry analysis. Caryophyllene oxide (15.

Traditional Knowledge, Use, and Management of Among the Mijikenda Community in Kilifi, Kenya.

Although Lam. (Moringaceae) is a multipurpose tree with remarkable nutritional and therapeutic benefits, it is undervalued and neglected in Kenya, as the local people associate it with famine and poverty. The present study aims to assess and document the traditional knowledge on use and management as well as production constraints of the species among the Mijikenda community in Kilifi County, Kenya.

Changes in Secondary Metabolites Content and Antioxidant Enzymes Activity in Leaves of Two L. Genotypes During Various Phenological Phases.

In addition to its fruit, the sweet cherry ( L.) has other parts that can be used as a source of compounds with beneficial biological activity. The content of these metabolites is affected by different inner and outer factors, often as a response to plant defense against various stresses.

Could Milkability Parameters Serve as a Reliable Tool to Predict the Morphology of Teat Structures and Their Milking-Induced Changes?

The study aimed to explore if milkability parameters could reliably predict the dimensions of teat structures and their milking-induced changes. Ultrasonography repeatedly measured the teat structures of 48 Holstein cows from mid to late lactation. We found that milking-induced changes in each structure are affected by different milkability parameters.

In Vitro and In Vivo Evaluation of the Fertilization Capacity of Frozen/Thawed Rooster Spermatozoa Supplemented with Different Concentrations of Trehalose.

The objective of this study was to evaluate the impact of the supplementation of varying concentrations of the impermeable disaccharide trehalose on the in vitro and in vivo fertilization capacity of cryopreserved rooster spermatozoa in the original Czech Golden Spotted Hen breed. The control trehalose concentration was 0 mM, while TRE50 (50 mM), TRE100 (100 mM), and TRE200 (200 mM) were used as experimental trehalose concentrations. The kinematic and functional parameters of frozen/thawed spermatozoa were evaluated in vitro using mobile computer-assisted sperm analysis and a flow cytometer.

Drivers of Seasonal Change of Avian Communities in Urban Parks and Cemeteries of Latin America.

Urban parks and cemeteries constitute hot spots of bird diversity in urban areas. However, the seasonal dynamics of their bird communities have been scarcely explored at large scales. This study aims to analyze the drivers of urban bird assemblage seasonality in urban parks and cemeteries comparing assemblages during breeding and non-breeding seasons in the Neotropical Region.

Rapid climate action is needed: comparing heat vs. COVID-19-related mortality.

The impacts of climate change on human health are often underestimated or perceived to be in a distant future. Here, we present the projected impacts of climate change in the context of COVID-19, a recent human health catastrophe. We compared projected heat mortality with COVID-19 deaths in 38 cities worldwide and found that in half of these cities, heat-related deaths could exceed annual COVID-19 deaths in less than ten years (at + 3.

Ensuring food sovereignty and nutritional sustainability in Egypt.

Understanding Egypt's dependence on wheat imports is crucial for enhancing food security and economic stability. This study aims to identify the extent of Egypt's wheat import dependency and recommend measures for increasing food self-sufficiency. We employed index analysis and an econometric model to analyze data sourced from the Food and Agriculture Organization (FAO), Observatory of Economic Complexity (OEC), Chicago Mercantile Exchange (CME), World Bank (WB), and Organisation for Economic Co-operation and Development (OECD).

Insight into the changes of European agriculture during the age of Baroque and enlightenment: Interdisciplinary survey of manor farmyard Švamberk (Czech Republic).

Following European exploration of the Americas in the late 15th century, new plants rapidly spread across Europe. Simultaneously, plants from Asia and Africa arrived. Initially, they were grown in ornamental gardens but later became integral to major production centres, significantly transforming European agriculture.