6 results match your criteria: "Cyprus Cancer Research Institute (CCRI)[Affiliation]"
Tumor associated macrophages in breast cancer progression: implications and clinical relevance.
Front Immunol
July 2024
Department of Translational Research and Precision Medicine, Cyprus Cancer Research Institute (CCRI), Nicosia, Cyprus.
Article Synopsis
- Macrophages are immune cells that can adapt their functions based on their environment, playing complex roles in cancer, particularly in breast cancer development and progression.
- Tumor-associated macrophages (TAMs) in the tumor microenvironment (TME) are linked to worse clinical outcomes, especially in aggressive breast cancer types like triple-negative breast cancer (TNBC).
- The review highlights how TAMs promote tumor development through mechanisms like enhancing cancer cell features and suggests understanding their roles may improve treatment strategies.
Protocol for mapping physiological DSBs using in-suspension break labeling in situ and sequencing.
STAR Protoc
June 2024
The Concern Foundation Laboratories, The Lautenberg Center for Immunology and Cancer Research, Department of Immunology and Cancer Research-IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel; Cyprus Cancer Research Institute (CCRI), Nicosia, Cyprus. Electronic address:
Physiological double-stranded breaks (DSBs) are a major source of genomic instability. Here, we present a protocol for mapping physiological DSBs by in-suspension break labeling in situ and sequencing (sBLISS) in a single-nucleotide resolution. We describe steps for cell fixation, labeling of DSBs, DNA isolation followed by in vitro transcription (IVT), reverse transcription, and library preparation.
View Article and Find Full Text PDFUnveiling the relationship between WWOX and BRCA1 in mammary tumorigenicity and in DNA repair pathway selection.
Cell Death Discov
March 2024
The Concern Foundation Laboratories, The Lautenberg Center for Immunology and Cancer Research, Department of Immunology and Cancer Research-IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Breast cancer is the leading cause of cancer-related deaths in women worldwide, with the basal-like or triple-negative breast cancer (TNBC) subtype being particularly aggressive and challenging to treat. Understanding the molecular mechanisms driving the development and progression of TNBC is essential. We previously showed that WW domain-containing oxidoreductase (WWOX) is commonly inactivated in TNBC and is implicated in the DNA damage response (DDR) through ATM and ATR activation.
View Article and Find Full Text PDFTOP1 and R-loops facilitate transcriptional DSBs at hypertranscribed cancer driver genes.
iScience
March 2024
The Concern Foundation Laboratories, The Lautenberg Center for Immunology and Cancer Research, Department of Immunology and Cancer Research-IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
DNA double-stranded breaks (DSBs) pose a significant threat to genomic integrity, and their generation during essential cellular processes like transcription remains poorly understood. In this study, we employ several techniques to map DSBs, R-loops, and topoisomerase 1 cleavage complex (TOP1cc) to comprehensively investigate the interplay between transcription, DSBs, topoisomerase 1 (TOP1), and R-loops. Our findings reveal the presence of DSBs at highly expressed genes enriched with TOP1 and R-loops.
View Article and Find Full Text PDFCorrection: WWOX promotes osteosarcoma development via upregulation of Myc.
Cell Death Dis
February 2024
The Concern Foundation Laboratories, The Lautenberg Center for Immunology and Cancer Research, Department of Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
WWOX promotes osteosarcoma development via upregulation of Myc.
Cell Death Dis
January 2024
The Concern Foundation Laboratories, The Lautenberg Center for Immunology and Cancer Research, Department of Immunology and Cancer Research, IMRIC, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
Article Synopsis
- Osteosarcoma is a rare and aggressive bone cancer that primarily affects younger populations, with poor outcomes and a tendency to spread to the lungs.
- The study utilized a mouse model to investigate the molecular mechanisms behind osteosarcoma by creating single and double knockouts of specific genes (Trp53 and Wwox) and tracking early tumor cell markers.
- Findings indicated that double-knockout cells showed tumor-like characteristics and upregulation of the Myc gene, suggesting it plays a role in the cancer's development, while inhibiting one of its targets (MCM7) reduced cell growth in these double-knockout young bone marrow cells.