Isolation, Identification, and Antimicrobial Susceptibilities of Bacteria from the Conjunctival Sacs of Dogs with Bacterial Conjunctivitis in Different Regions of Wuhan, China.

In order to investigate the bacterial species present in the conjunctival sacs of dogs with bacterial conjunctivitis in Wuhan (Hongshan District, Wuchang District, Jiangxia District, and Huangpi District) and their resistance to aminoglycoside antibiotics, samples of conjunctival sac secretions were collected from 56 dogs with bacterial conjunctivitis in various regions of Wuhan. Drug susceptibility testing for aminoglycoside antibiotics was performed on the most commonly isolated gram-positive and gram-negative bacteria. The expression of two aminoglycoside modifying enzyme genes, and , and three 16S rRNA methyltransferase genes, , and , were analyzed by PCR.

Effects of Long-Term Serum Starvation on Autophagy, Metabolism, and Differentiation of Porcine Skeletal Muscle Satellite Cells.

This study investigated the effects of long-term serum starvation on autophagy, metabolism, and differentiation of porcine skeletal muscle satellite cells (SMSCs) and elucidated the role of autophagy in skeletal muscle development. Our findings provide a theoretical basis for improving meat production in domestic pigs. The SMSCs isolated and preserved in our laboratory were revived and divided into six groups based on the culture medium serum concentration to simulate varying levels of serum starvation: 20% serum (control group), 15% serum (mild serum starvation group), 5% serum (severe serum starvation group), and their autophagy inhibition groups supplemented with 3-methyladenine.

Identification of Health Needs in Ukrainian Refugees Seen in a Primary Care Facility in Tenerife, Spain.

Background: Ukrainian refugees fleeing the conflict between Russia and Ukraine may face significant challenges to their physical, psycho-emotional, social, and spiritual wellbeing.

Aim: To identify the health needs of Ukrainian refugees seen in primary care facilities in Tenerife, Canary Islands, Spain.

Methods: A mixed-methods design was employed.

Correlation Between Infection and the Nasal Mycobiota in a Population of Free-Ranging Koalas () in New South Wales, Australia.

Cryptococcosis is a fungal disease in humans and animals, caused by the and species complexes. Clinical cryptococcosis primarily manifests as upper respiratory tract disease; however, dissemination to other organs, particularly the brain, can occur. Nasal colonisation and subclinical cryptococcosis are common in koalas () due to their shared environmental niche with : trees.

Ex Situ Conservation, DNA Barcoding and Enzymatic Potential Evaluation of Macrofungi (Basidiomycota, Ascomycota) from Vietnam.

The diversity and resource potential of macroscopic fungi in tropical regions remain understudied. Vietnam, being in a biodiversity hotspot, has a large number of new fungal species that are of interest for biotechnology and medicine. The presence of a large number of protected areas in Vietnam creates favorable opportunities for the study and ex situ conservation of tropical biodiversity.

Toward an Efficient Differentiation of Two Strains Through Mass Spectrometry for Fungal Biotyping.

Considering that fungi display a great morphological, ecological, metabolic, and phylogenetic diversity, their taxonomic identification is extremely important because it helps us establish important information about each species and its possible biochemical and ecological roles. Traditionally, the identification of fungi at the species level has been carried out with molecular tools such as DNA sequencing, but it still represents a huge challenge today due to the heterogeneity of the fungal kingdom, making the task of identification a complex and difficult process. Biotyping, a type of chemotaxonomy, has been developed in the field of the identification/differentiation and classification of micro-fungi through tools such as mass spectrometry (MS).

Assessing the Measurement Properties of the Test of Gross Motor Development-3 Using the COSMIN Methodology-A Systematic Review.

This study aimed to systematically review the measurement properties of the Test of Gross Motor Development-3 (TGMD-3) using the COSMIN methodology. A search of four databases (PubMed, EMBASE, Web of Science, CINAHL) identified 23 relevant studies. The methodological quality of the studies was assessed using the COSMIN risk of bias checklist; the measurement properties of the TGMD-3 were evaluated by the COSMIN quality criteria; and the quality of the evidence was rated using a modified GRADE approach.

Relationship Between Participative Decision-Making Within an Organization and Employees' Cognitive Flexibility, Creativity, and Voice Behavior.

The existing literature predominantly examines the direct effects of participative decision-making, often overlooking the mechanisms and processes that mediate or moderate its outcomes. This study addresses this gap by investigating the impact of participative decision-making on employees' cognitive flexibility, creativity, and voice behavior. Specific contradictions and gaps in prior research are highlighted, particularly the limited understanding of how these variables interact.

Effects of Failure Acceptance, Entrepreneurial Orientation, and Social Safety Net on Entrepreneurial Intention: A Moderated Mediation Analysis of Korean Employees.

This study aims to examine innovation using an entrepreneurial event model by exploring the effects of failure acceptance, entrepreneurial orientation, and social safety nets on entrepreneurial intention. A survey was conducted with employees in South Korea to collect data, which were analyzed statistically using SPSS version 27.0 and Macro 4.

"Direct Me or Leave Me": The Effect of Leadership Style on Stress and Self-Efficacy of Healthcare Professionals.

This study aims to investigate the influence of leadership on the self-efficacy of healthcare professionals. Additionally, it seeks to explore whether stress mediates the relationship between leadership and self-efficacy. Specifically, our study is focused on both transactional leadership and laissez-faire leadership, which are commonly practiced by healthcare professionals due to the settings of healthcare environments.

Behavioral Emotion Regulation Strategies and Symptoms of Psychological Distress Among Turkish University Students.

The purpose of this study was to examine the association between behavioral emotion regulation strategies and symptoms of depression, anxiety, and stress among Turkish university students. Participants consisted of 633 students continuing their university education in two different universities in Türkiye. Participants completed a data collection tool comprising a Sociodemographic Information Form, the Behavioral Emotion Regulation Questionnaire, and the Depression Anxiety Stress Scales-21.

Barriers and Factors Affecting Nursing Communication When Providing Patient Care in Jeddah.

Effective communication between nurses and patients plays a crucial role in the delivery of quality healthcare services, especially when caring for patients from diverse cultural backgrounds. It fosters trust, understanding, and collaboration and contributes to better health outcomes and satisfactory nurse-patient relationships. The aim of the study is to assess the factors and barriers affecting nurses' communication when providing care for patients from diverse cultural backgrounds in Jeddah, Saudi Arabia.

Decoding Urinary Tract Infection Trends: A 5-Year Snapshot from Central Portugal.

Introduction: This study analyzes urinary tract infections (UTIs) in a hospital in Central Portugal over a five-year period, focusing on bacterial prevalence, patient demographics, and antibiotic resistance patterns. This investigation aims to provide insights that can guide improved infection control and treatment strategies.

Methods: A total of 6161 positive urine cultures collected over five years were examined, with particular emphasis on 2019 due to a peak in infection rates.

Bone Marrow Stromal Cells Generate a Pro-Healing Inflammasome When Cultured on Titanium-Aluminum-Vanadium Surfaces with Microscale/Nanoscale Structural Features.

The surface topography and chemistry of titanium-aluminum-vanadium (Ti6Al4V) implants play critical roles in the osteoblast differentiation of human bone marrow stromal cells (MSCs) and the creation of an osteogenic microenvironment. To assess the effects of a microscale/nanoscale (MN) topography, this study compared the effects of MN-modified, anodized, and smooth Ti6Al4V surfaces on MSC response, and for the first time, directly contrasted MN-induced osteoblast differentiation with culture on tissue culture polystyrene (TCPS) in osteogenic medium (OM). Surface characterization revealed distinct differences in microroughness, composition, and topography among the Ti6Al4V substrates.

Introducing ActiveInference.jl: A Julia Library for Simulation and Parameter Estimation with Active Inference Models.

We introduce a new software package for the Julia programming language, the library ActiveInference.jl. To make active inference agents with Partially Observable Markov Decision Process (POMDP) generative models available to the growing research community using Julia, we re-implemented the pymdp library for Python.

miR-223 and Chromogranin A Affect Inflammatory Immune Cell Activation in Liver Metastasis of Neuroendocrine Neoplasms.

Neuroendocrine neoplasms (NENs) are a diverse group originating from endocrine cells/their precursors in pancreas, small intestine, or lung. The key serum marker is chromogranin A (CgA). While commonly elevated in patients with NEN, its prognostic value is still under discussion.

Fluorescence Lifetime Imaging of NAD(P)H in Patients' Lymphocytes: Evaluation of Efficacy of Immunotherapy.

Background: The wide variability in clinical responses to anti-tumor immunotherapy drives the search for personalized strategies. One of the promising approaches is drug screening using patient-derived models composed of tumor and immune cells. In this regard, the selection of an appropriate in vitro model and the choice of cellular response assay are critical for reliable predictions.

The Potential Clinical Utility of the Customized Large Language Model in Gastroenterology: A Pilot Study.

The large language model (LLM) has the potential to be applied to clinical practice. However, there has been scarce study on this in the field of gastroenterology. Aim: This study explores the potential clinical utility of two LLMs in the field of gastroenterology: a customized GPT model and a conventional GPT-4o, an advanced LLM capable of retrieval-augmented generation (RAG).

Antibiotic exposure enriches streptococci carrying resistance genes in periodontitis plaque biofilms.

Background: Periodontitis is not always satisfactorily treated with conventional scaling and root planing, and adjunctive use of antibiotics is required in clinical practice. Therefore, it is important for clinicians to understand the diversity and the antibiotic resistance of subgingival microbiota when exposed to different antibiotics.

Materials And Methods: In this study, subgingival plaques were collected from 10 periodontitis patients and 11 periodontally healthy volunteers, and their microbiota response to selective pressure of four antibiotics (amoxicillin, metronidazole, clindamycin, and tetracycline) were evaluated through 16S rRNA gene amplicon and metagenomic sequencing analysis.

Phenotypic and genetic extended spectrum beta lactamase profiles of bacterial isolates from ICU in tertiary level hospital in Kenya.

Background: Bacterial infections in the Intensive Care Units are a threat to the lives of critically ill patients. Their vulnerable immunity predisposes them to developing bacteria-associated sepsis, deteriorating their already fragile health. In the face of increasing antibiotics resistance, the problem of bacterial infection in ICU is worsening.

Boy's love fans versus non-fans in the sexual identity and neural response in the digital age's young females.

With the omnipresence of online social media, Boys' Love (BL) culture has found a burgeoning audience among young females. However, we know very little about the audience of this online cultural phenomena, also the potential implications of BL culture to female remain under-explored. Study 1 conducted a survey to investigate the BL audience's demography data and attitudes to homosexual ect.

Therapeutic efficacy of intra-articular injection of human adipose-derived mesenchymal stem cells in a sheep model of knee osteoarthritis.

Background: Mesenchymal stem cells have great potential for repairing articular cartilage and treating knee osteoarthritis (KOA). Nonetheless, little is known about the efficacy of human adipose-derived mesenchymal stem cells (haMSCs) for KOA in large animal models.

Methods: This study evaluated the therapeutic efficacy of haMSCs in knee articular cartilage repair in a sheep model of KOA.

Understanding malaria treatment patronage from informal healthcare providers in Nigerian urban settlements: insights from community members and providers.

Background: Informal Healthcare Providers (IHCPs), including Proprietary Patent Medicine Vendors (PPMVs), drug peddlers, traditional healers, and herbal drug sellers are often the first choice for malaria treatment, especially in urban slums. Unplanned urbanization significantly impacts malaria transmission by creating cities with inadequate safety nets and healthcare access, increasing reliance on IHCPs. While the World Health Organization recognizes IHCP's crucial role and emphasizes integrating them into formal healthcare for improved malaria care, they lack requisite training in malaria management and operate outside official regulations, raising concerns about the quality of care they provide.

Effect of selected Indonesian plants on Giardia intestinalis in an experimental in vitro model.

Background: This study evaluated in vitro antigiardial activity in four Indonesian plants (Archidendron fagifolium, Diospyros sumatrana, Piper betle and Shorea sumatrana) extracted in methanol, methanol-tetrahydrofuran, and water. These plants exhibiting promising anti-parasitic activity were selected on the basis of collected behavioral data and their ability to decrease parasite load in Sumatran orangutans. Extracts of Arabidopsis thaliana, a plant routinely used as a laboratory model in research, were used as a negative control.

Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China.

Background: Diabetic foot ulcers (DFUs) are characterized by dynamic wound microbiome, the timely and accurate identification of pathogens in the clinic is required to initiate precise and individualized treatment. Metagenomic next-generation sequencing (mNGS) has been a useful supplement to routine culture method for the etiological diagnosis of DFUs. In this study, we utilized a routine culture method and mNGS to analyze the same DFU wound samples and the results were compared.