Flow cytometry-based diagnostic approach for inborn errors of immunity: experience from Algeria.

Purpose: In this study, we retrospectively reviewed the use of flow cytometry (FCM) in the diagnosis of inborn errors of immunity (IEIs) at a single center in Algeria. Sharing insights into our practical experience, we present FCM based diagnostic approaches adapted to different clinical scenarios.

Methods: Between May 2017 and February 2024, pediatric and adult patients presenting with clinical features suggestive of immunodeficiency were subjected to FCM evaluation, including lymphocyte subset analysis, detection of specific surface or intracellular proteins, and functional analysis of immune cells.

Assessing hand and global disability in a cohort of Algerian patients with systemic sclerosis: Construct validities of the Arab Hand Function Index and the Arabic Health Assessment Questionnaire.

Objective: The study aimed to assess the construct validity of the Arab Hand Function Index and the Arabic Health Assessment Questionnaire in Algerian patients with systemic sclerosis.

Methods: Consecutive Algerian patients who fulfilled the American College of Rheumatology/European League Against Rheumatism criteria for systemic sclerosis were included. In addition to disease characteristics, global disability and hand disability were assessed using the Arabic Health Assessment Questionnaire and the Arab Hand Function Index, respectively.

Neoadjuvant endocrine therapy with or without palbociclib in low-risk patients: a phase III randomized double-blind SAFIA trial.

Background: The most prevalent subtype of breast cancer (BC) is luminal hormonal-positive breast cancer. The neoadjuvant chemotherapy regimens have side effects, emphasizing the need to identify new startegies.

Objective: Analyze the complete pathologic response (pCR) rate and overall response in a low-risk hormone-positive subset of patients receiving neoadjuvant hormone treatment (NAHT) with or without Palbociclib (a CDK4/CDK6 inhibitor) to boost NAHT effectiveness.

Stereological Evidence of Non-Selective Hippocampal Neurodegeneration, IGF-1 Depletion, and Behavioral Deficit following Short Term Bilateral Adrenalectomy in Wistar Rats.

The development of animal models to study cell death in the brain is a delicate task. One of the models, that was discovered in the late eighties, is the induction of neurodegeneration through glucocorticoid withdrawal by adrenalectomy in albino rats. Such a model is one of the few noninvasive models for studying neurodegeneration.

Air Pollution and Health Risk: Intelligent Mapping.

Air pollution by chemical or bacteriological pollutants is not limited to a geographical area at the discrete border. Also, factors taken into account in the risk analysis such as patient age, seasons, socio-economic background or race are far from being necessary as discrete values. Often used analysis tools are limited to statistical analyzes.

Preparation, DFT calculations, docking studies, antioxidant, and anticancer properties of new pyrazole and pyridine derivatives.

Seven novel pyrazole derivatives (4a-g) and four novel starting compounds incorporating substituted pyridine moieties were synthesized successfully. Cell viability assay for the tested compounds was performed, and the inhibitory concentrationlogarithmic 50 (LogIC ) values of the compounds were calculated after a 24-h treatment. Four of the examined compounds (3d, 3g, 4f, and 4g) showed comparable cytotoxic activity against CaCo-2 compared to the standard drug docetaxel at 0.

Distribution of ABO alleles in the Northeast Algerian population.

Objectives: The study of ABO polymorphism has contributed to the understanding of human genetic diversity. The objective of our work is to determine the phenotypic and allelic frequencies of the ABO system in the population of the Algerian Northeast, and to make a comparative study with other national and world populations.

Methods: The study included 10,208 blood donors, originating from the Northeast of Algeria, the ABO grouping was carried out by serological techniques on plate and gel card, the comparison of the ABO allelic frequencies, and the genetic distances between the populations, were carried out respectively by using the R software, and the SPSS version 20 software.

Response to Induction Neoadjuvant Hormonal Therapy Using Upfront 21-Gene Breast Recurrence Score Assay-Results From the SAFIA Phase III Trial.

Purpose: Luminal, human epidermal growth factor receptor 2-negative breast cancer represents the most common subtype of breast malignancies. Neoadjuvant strategies of operable breast cancer are mostly based on chemotherapy, whereas it is not completely understood which patients might benefit from neoadjuvant hormone therapy (NAHT).

Materials And Methods: The SAFIA trial is a prospective multicenter, international, double-blind, neoadjuvant phase III trial, using upfront 21-gene Oncotype DX Breast Recurrence Score assay (recurrence score [RS] < 31) to select operable luminal human epidermal growth factor receptor 2-negative patients, for induction hormonal therapy HT (fulvestrant 500 mg with or without goserelin) before randomly assigning responding patients to fulvestrant 500 mg (with or without goserelin) plus either palbociclib (cyclin-dependent kinase 4/6 inhibitor) or placebo.

Synthesis and anticancer properties of novel hydrazone derivatives incorporating pyridine and isatin moieties.

Nine novel hydrazone derivatives (4a-i) incorporating pyridine and isatin moieties were synthesized through one-pot, four-component heterocyclic condensation reactions. The structures of all new compounds (2a-e, 3a, 3c-e, and 4a-e) were identified by H nuclear magnetic resonance (NMR), C NMR, and Fourier-transform infrared spectroscopic techniques and elemental analysis. Cell viability assays for the tested hydrazone derivatives were performed and the log IC values of the compounds were calculated after a 24-h treatment.

Combining photocatalytic process and biological treatment for Reactive Green 12 degradation: optimization, mineralization, and phytotoxicity with seed germination.

In this study, we show that the combination of a photocatalytic process (as a pretreatment step) combined with the conventional biological treatment of wastewaters can improve the process and achieve satisfactory efficiency. In this context, Reactive Green 12 (RG-12) solutions were photocatalytically pretreated using TiO-impregnated polyester as supported catalyst under UV light in batch reactor. Photocatalysis as pretreatment (during 4 and 8 h of irradiation) was combined with 7 days of aerobic biological treatment using activated sludge.

Box-Wilson design modeling of photocatalytic degradation of industrial dye and wastewater in semi-pilot solar photoreactor.

This work focuses on the treatment of a dye solution, C.I. Basic Blue 41 (BB41), and industrial wastewater by UV/TiO photocatalytic process using aqueous catalyst suspensions of titanium dioxide (TiO), Degussa P25.

A simplified table using validated diagnostic criteria is effective to improve characterization of colorectal polyps: the CONECCT teaching program.

Accurate real-time endoscopic characterization of colorectal polyps is key to choosing the most appropriate treatment. Mastering the currently available classifications is challenging. We used validated criteria for these classifications to create a single table, named CONECCT, and evaluated the impact of a teaching program based on this tool.

Synthesis, molecular docking studies, and biological evaluation of novel alkyl bis(4-amino-5-cyanopyrimidine) derivatives.

A series of bis(4-amino-5-cyano-pyrimidines) was synthesized and evaluated as dual inhibitors of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). To further explore the multifunctional properties of the new derivatives, their antioxidant and antibacterial activities were also tested. The results showed that most of these compounds could effectively inhibit AChE and BChE.

Report on spp. (Diptera, Gasterophilidae) of Horses in Algeria: Prevalence, Intensity, and Monthly Variations.

The composition, prevalence, and seasonal dynamic of species were studied at the slaughterhouse of Constantine region (East Algeria) in 128 horses over an 18 months period. Our survey revealed that 124 (96.9%) horses were infected with a mean intensity of 161 larvae of spp.

Assessment of water quality at the largest dam in Algeria (Beni Haroun Dam) and effects of irrigation on soil characteristics of agricultural lands.

Beni Haroun Dam is a vital water resource for both potable water and irrigation in eastern Algeria. This study assessed water quality at the dam and investigated the impacts of this water on some soil characteristics of irrigated lands. The physicochemical parameters of the water were measured monthly at eight sites and related to soil characteristics sampled from six irrigated areas, which were compared to non-irrigated soils.

The multi-ethnic global lung initiative 2012 (GLI-2012) norms reflect contemporary adult's Algerian spirometry.

Background: The validation of the multi-ethnic GLI-2012 spirometric norms has been debated in several countries. However, its applicability in Algeria has not been verified.

Aim: To ascertain how well the GLI-2012 norms fit contemporary adult Algerian spirometric data.

A cascade synthesis, in vitro cholinesterases inhibitory activity and docking studies of novel Tacrine-pyranopyrazole derivatives.

In this work, we describe the preparation of some new Tacrine analogues modified with a pyranopyrazole moiety. A one-pot multicomponent reaction of 3-methyl-1H-pyrazol-5(4H)-one, aryl(or hetero)aldehydes, malononitrile and cyclohexanone involving a Friedländer condensation led to the title compounds. The synthesized heterocyclic analogues of this molecule were evaluated in vitro for their AChE and BChE inhibitory activities in search for potent cholinesterase enzyme inhibitors.

Influence of waist circumference on blood pressure status in non-obese adolescents.

Objectives To check whether excess in abdominal adiposity and metabolic factors were associated with blood pressure abnormalities in non-obese adolescents. Methods We randomly selected 1100 adolescent students, aged 12-18 years, from schools and classrooms in the city of Constantine, Algeria. Among them 179 were overweight and 51 were obese (IOTF criteria).

Intravenous thrombolysis for patients with in-hospital stroke onset: propensity-matched analysis from the Safe Implementation of Treatments in Stroke-East registry.

Background And Purpose: Recent cross-sectional study data suggest that intravenous thrombolysis (IVT) in patients with in-hospital stroke (IHS) onset is associated with unfavorable functional outcomes at hospital discharge and in-hospital mortality compared to patients with out-of-hospital stroke (OHS) onset treated with IVT. We sought to compare outcomes between IVT-treated patients with IHS and OHS by analysing propensity-score-matched data from the Safe Implementation of Treatments in Stroke-East registry.

Methods: We compared the following outcomes for all propensity-score-matched patients: (i) symptomatic intracranial hemorrhage defined with the safe implementation of thrombolysis in stroke-monitoring study criteria, (ii) favorable functional outcome defined as a modified Rankin Scale (mRS) score of 0-1 at 3 months, (iii) functional independence defined as an mRS score of 0-2 at 3 months and (iv) 3-month mortality.

IpiRId: Integrative approach for piRNA prediction using genomic and epigenomic data.

Many computational tools have been proposed during the two last decades for predicting piRNAs, which are molecules with important role in post-transcriptional gene regulation. However, these tools are mostly based on only one feature that is generally related to the sequence. Discoveries in the domain of piRNAs are still in their beginning stages, and recent publications have shown many new properties.

Usefulness of techniques based on real time PCR for the identification of onychomycosis-causing species.

Onychomycosis (OM) is a nail infection caused mainly by dermatophyte species but other species of yeast and moulds are frequently involved as well. Classical diagnosis has limitations thus empirical treatment is common. The usefulness of different real time PCR (RT-PCR) assays for identifying species causing OM was assessed in samples from seventy patients and fifteen controls.

In Silico Prediction of RNA Secondary Structure.

The secondary structure of an RNA molecule represents the base-pairing interactions within the molecule and fundamentally determines its overall structure. In this chapter, we overview the main approaches and existing tools for predicting RNA secondary structures, as well as methods for identifying noncoding RNAs from genomic sequences or RNA sequencing data. We then focus on the identification of a well-known class of small noncoding RNAs, namely microRNAs, which play very important roles in many biological processes through regulating post-transcriptionally the expression of genes and which dysregulation has been shown to be involved in several human diseases.

Arrested development of abomasal trichostrongylid nematodes in lambs in a steppe environment (North-Eastern Algeria).

Arrested development of abomasal trichostrongylid nematodes was studied in 30 permanent grazing lambs on a large farm in the North-East of Algeria. The steppe climate has cold winters and hot and dry summers. The lambs were monitored monthly for gastrointestinal nematodes using nematode faecal egg counts, from February 2008 to February 2009.

Thermal analysis of Al + 0.1% CNT ribbon.

The objective of this work is a dilatometric study of Al + 0.1% of multiwall carbon nanotubes nanocomposite material (NCM) in three directions: X - parallel to the rolling direction; Y - perpendicular to the rolling direction and (Z) perpendicular to the ribbon plane. NCM specimens were made in the form of a 0.