A higher spectral range of beetle bioluminescence with infraluciferin.

Coleopteran bioluminescence is unique in that beetle luciferases emit colors ranging between green (ca.550 nm) and red (ca.600 nm), including intermediate colors such as yellow and orange, allowing up to 3 simultaneous parameters to be resolved with natural luciferin (-LH).

Outsider at home: Reading Babasaheb Ambedkar as a radical, decolonial psychologist.

Objectives: Known primarily as the architect of the constitution of India, Babasaheb Ambedkar was also a human rights lawyer, an economist, a social justice advocate, and a polymath. Yet his story is often overlooked in favor of national leaders such as Gandhi. This study highlights Ambedkar as a visionary who called for a radical and new psychology of self that was anchored in ideas of social justice, equity and full participation.

Seating in Doctors' Waiting Rooms: Has COVID-19 Changed Our Choices?

Objective: This study investigates preference for five different seating arrangements (e.g., rows, clusters) in a doctor's office waiting room, whether these reduce stress and improve judgments of medical care, and how such choices may have changed over the pandemic (2013 vs.

AlphaFold2 and RoseTTAFold predict posttranslational modifications. Chromophore formation in GFP-like proteins.

AlphaFold2 and RoseTTAfold are able to predict, based solely on their sequence whether GFP-like proteins will post-translationally form a chromophore (the part of the protein responsible for fluorescence) or not. Their training has not only taught them protein structure and folding, but also chemistry. The structures of 21 sequences of GFP-like fluorescent proteins that will post-translationally form a chromophore and of 23 GFP-like non-fluorescent proteins that do not have the residues required to form a chromophore were determined by AlphaFold2 and RoseTTAfold.

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Outbreak at a College With High Coronavirus Disease 2019 (COVID-19) Vaccination Coverage-Connecticut, August 2021-September 2021.

Background: During August 2021-September 2021, a Connecticut college experienced a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Delta variant outbreak despite high (99%) vaccination coverage, indoor masking policies, and twice-weekly testing. The Connecticut Department of Public Health investigated characteristics associated with infection and phylogenetic relationships among cases.

Methods: A case was a SARS-CoV-2 infection diagnosed by a viral test during August 2021-September 2021 in a student.

Teaching to Empower: Leveraging the Neuroscience of Now to Help Students Become Self-Regulated Learners.

In his book Descartes' Error, neurologist Antonio Damasio argues that humans do not make decisions by relying exclusively on the rational or reason-oriented parts of their brain (2008). Evidence from patients with brain damage reveal that our abilities to reason and make decisions are greatly influenced by our emotions (Damasio et al., 1990; Saver and Damasio, 1991).

Context and the Perceived Fairness of Price Increases Coming out of COVID-19.

Objective: As the U.S. economy responds to prolonged COVID-19 disruptions, it is important to understand what factors affect the perceived fairness of pandemic-driven price increases.

The downsizing of gigantic scales and large cells in the genus Mallomonas (Synurales, Chrysophyceae).

Mallomonas is the largest and most speciose genus within the Synurales, a monophyletic clade of siliceous scale-bearing organisms within the class Chrysophyceae. The genus consists of unicellular, motile, photosynthetic organisms found in freshwater localities worldwide. Mallomonas diverged from other synurophytes during the lower Cretaceous at approximately 130 Ma.

Systematic Comparison of Beetle Luciferase-Luciferin Pairs as Sources of Near-Infrared Light for In Vitro and In Vivo Applications.

Luciferases catalyze light-emitting reactions that produce a rainbow of colors from their substrates (luciferins), molecular oxygen, and often additional cofactors. These bioluminescence (BL) systems have afforded an incredible variety of basic research and medical applications. Driven by the importance of BL-based non-invasive animal imaging (BLI) applications, especially in support of cancer research, new BL systems have been developed by engineering beetle luciferase (Luc) variants and synthetic substrate combinations to produce red to near-infrared (nIR) light to improve imaging sensitivity and resolution.

Prospective analyses of sex/gender-related publication decisions in general medical journals: editorial rejection of population-based women's reproductive physiology.

Objective: To assess whether editorial desk rejection at general medical journals (without peer review) of two clinical research manuscripts may relate to author gender or women's physiology topics. Given evidence for bias related to women in science and medicine, and editorial board attitudes, our hypothesis was that submissions by women authors, on women's reproductive, non-disease topics received differential editorial assessment.

Design: A prospective investigation of publications, author gender and topics in general medical journals in two issues following the editorial rejections of two clinical research manuscripts by five major English-language general medical journals.

The GLT-1 enhancer clavulanic acid suppresses cocaine place preference behavior and reduces GCPII activity and protein levels in the rat nucleus accumbens.

The β-lactam antibiotic ceftriaxone (CTX) is a glutamate transporter subtype 1 (GLT-1) enhancer that reduces cocaine reinforcing efficacy and relapse in rats, but pharmacokinetic liabilities limit translational utility. An attractive alternative is clavulanic acid (CLAV), a structurally related β-lactamase inhibitor and component of FDA-approved Augmentin. CLAV retains the GLT-1 enhancing effects of CTX but displays greater oral bioavailability, brain penetrability and negligible antibacterial activity.

A first account of the heterotrophic eukaryote Rabdiophrys Rainer from the fossil record and description of a new species from an ancient Eocene Arctic freshwater lake.

Rotosphaerids are unicellular, heterotrophic, eukayotic protists that have filopodia, an exterior covering consisting of highly ornamented siliceous scales, and are classified in the Rotosphaerida within the opistokont lineage. Given their appearance as relatively large spherical cells with protruding filopodia and a silica scale covering, they are often mistaken for centrohelid heliozoans. Even though these organisms are widely distributed in both marine and freshwater environments, many species are rarely reported, and none have been reported from the fossil record.

Why Are Gly31, Gly33, and Gly35 Highly Conserved in All Fluorescent Proteins?

Green fluorescent protein (GFP)-like fluorescent proteins have been found in more than 120 species. Although the proteins have little sequence identity, Gly31, 33, and 35 are 87, 100, and 95% conserved across all species, respectively. All GFP-like proteins have a β-barrel structure composed of 11 β-sheets, and the 3 conserved glycines are located in the second β-sheet.

Variability in environmental persistence but not per capita transmission rates of the amphibian chytrid fungus leads to differences in host infection prevalence.

Heterogeneities in infections among host populations may arise through differences in environmental conditions through two mechanisms. First, environmental conditions may alter host exposure to pathogens via effects on survival. Second, environmental conditions may alter host susceptibility, making infection more or less likely if contact between a host and pathogen occurs.

Self-defining memories-Narrative features in relation to adaptive and maladaptive personality traits (replication and extension of Blagov & Singer, 2004).

Objective: Self-defining memories (SDMs) are units of life-story analysis, whose features resemble elements from narrative identity's factorial structure. To bridge narrative-identity and personality-trait domains, we conducted a replication and extension of prior research.

Method: We linked four SDM features - affect, specificity, meaning making, and content - to the Big Three trait domains of personality and psychopathology in a small sample that was well-powered for multilevel modeling (133 participants, 1330 SDMs).

Attentional capture in goal-directed action during childhood, adolescence, and early adulthood.

Attentional capture occurs when salient but task-irrelevant information disrupts our ability to respond to task-relevant information. Although attentional capture costs have been found to decrease between childhood and adulthood, it is currently unclear the extent to which such age-related changes reflect an improved ability to recover from attentional capture or to avoid attentional capture. In addition, recent research using hand-tracking techniques with adults indicates that attentional capture by a distractor can generate response activations corresponding to the distractor's location, consistent with action-centered models of attention.

A Mobile Health Breast Cancer Educational and Screening Intervention Tailored for Low-Income, Uninsured Latina Immigrants.

To investigate the efficacy of mobile health (mHealth) intervention strategies that delivered either personalized, culturally, and linguistically tailored cell phone voice messages or text messages related to breast cancer and prevention, compared to the control group, to determine which strategy is more likely to increase breast cancer knowledge and screening mammography among low-income Latina immigrants. This randomized controlled trial assigned 256 Latina immigrants 40 years of age or older to one of three groups: an automated cell phone voice message group, an automated text message group, or the control group (mail). The mHealth intervention employed a comprehensive approach that included breast cancer and prevention education and free mammography screening.

Asymmetric cross-strain protection for amphibians exposed to a fungal-metabolite prophylactic treatment.

Chytridiomycosis, an infectious disease of amphibians caused by the fungal pathogen (Bd), poses an imminent conservation threat. The global spread of Bd has led to mass mortality events in many amphibian species, resulting in at least 90 species' extinctions to date. Exposure to Bd metabolites (i.

Dendritic cells maintain anti-tumor immunity by positioning CD8 skin-resident memory T cells.

Tissue-resident memory (T) T cells are emerging as critical components of the immune response to cancer; yet, requirements for their ongoing function and maintenance remain unclear. APCs promote T cell differentiation and re-activation but have not been implicated in sustaining T cell responses. Here, we identified a novel role for dendritic cells in supporting T to melanoma.

Adolescent and young adult childhood cancer survivors' preferences for screening and education of sexual function.

Background: Sexual dysfunction (SD) is a common yet underrecognized concern among childhood cancer survivors (CCS). CCS who are now adolescent and young adult (AYA-CCS) identify SD as an unmet need. This study sought to explore AYA-CCS preferences on how, when, where, and by whom SD-focused communication should occur.

Predictors and moderators of marijuana and heavy alcohol use outcomes in adolescents treated for co-occurring substance use and psychiatric disorders in a randomized controlled trial.

Background: The current study identifies predictors and moderators of substance use outcomes for 111 adolescents with co-occurring substance use and psychiatric disorders who participated in a randomized controlled trial that compared the effectiveness of two home-based treatments: an integrated cognitive behavioral therapy (I-CBT) protocol, in which masters-level clinic staff received intensive training and ongoing supervision in the use of this protocol versus a treatment-as-usual (TAU) comparison condition in which therapists received a continuing education-style CBT workshop in the same protocol.

Method: The study conducted exploratory predictor and moderator analyses of marijuana and heavy alcohol use outcomes using candidate variables across four domains of psychological characteristics: adolescent substance use, adolescent psychiatric symptoms, parent, and family.

Results: Regardless of treatment condition, low parental monitoring at baseline, as assessed by a videotaped interaction task, but not self-report, predicted greater percentage of marijuana use and heavy alcohol use days over the 6-month follow-up period.

A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase.

A novel bioluminescent Monoacylglycerol lipase (MAGL) substrate 6-O-arachidonoylluciferin, a D-luciferin derivative, was synthesized, physico-chemically characterized, and used as highly sensitive substrate for MAGL in an assay developed for this purpose. We present here a new method based on the enzymatic cleavage of arachidonic acid with luciferin release using human Monoacylglycerol lipase (MAGL) followed by its reaction with a chimeric luciferase, PLG2, to produce bioluminescence. Enzymatic cleavage of the new substrate by MAGL was demonstrated, and kinetic constants Km and Vmax were determined.

Maximum precision estimation for a step-stress model using two-stage methodologies.

In this paper, we consider a two-stage sequential estimation procedure to estimate the parameters of a cumulative exposure model under an accelerated testing scenario. In particular, we focus on a step-stress model where the stress level changes after a pre-specified number of failures occur, which is also random. This is termed as a 'random stress change time' in the literature.

Graphical models for mean and covariance of multivariate longitudinal data.

Joint mean-covariance modeling of multivariate longitudinal data helps to understand the relative changes among multiple longitudinally measured and correlated outcomes. A key challenge in the analysis of multivariate longitudinal data is the complex covariance structure. This is due to the contemporaneous and cross-temporal associations between multiple longitudinal outcomes.

Pediatric oncology clinician communication about sexual health with adolescents and young adults: A report from the children's oncology group.

Background: Sexual health (SH) is an important concern for adolescents and young adults (AYAs). This study determined current SH communication practices, barriers, and additional resources needed among pediatric oncology clinicians who treat AYAs.

Methods: A cross-sectional survey was developed by the Children's Oncology Group (COG) AYA Committee and sent to pediatric oncologists (n = 1,987; 85.