Long-term exposure to bisphenol-A causes oxidative stress-related alterations at the genetic and cellular levels in the mature ovary of adult zebrafish.

Bisphenol-A (BPA) is categorized as a major endocrine-disrupting chemical (EDC) used to manufacture many plastic products. BPA affects reproductive performance and promotes infertility by causing hormonal imbalance, mitochondrial dysfunction, and altered gene expression. The present investigation aimed to evaluate the effects of BPA exposure for 28 days on the activity or level of antioxidant response elements (AREs), mRNA expressions of antioxidant genes, and histomorphological changes in the ovary of adult zebrafish.

Metagenomic Analysis Reveals the Complex Microbial Landscape of Market Chicken Meat.

Unlabelled: The safety of meat from a microbiological standpoint is of paramount concern to public health, given the potential for bacterial contaminants to grow and persist during processing and storage. To address this issue, a culture-independent approach targeting the V3-V4 region of the gene was utilized to investigate the inherent bacterial communities present in 10 chicken meat samples obtained from retail markets. Amplicons were sequenced using the Illumina MiSeq platform, and unique amplicon sequence variants (ASVs) were identified using the DADA2 pipeline.

Profiling of antimicrobial resistance genes from Staphylococcus pseudintermedius isolated from dogs with pyoderma using whole genome sequencing.

Staphylococcus pseudintermedius is considered as a most common cause of canine pyoderma. Antimicrobial resistance is considered as a global public health concern. The main aim of the study was to evaluate the distribution of Antimicrobial resistance genes in S.

Phenotypic and genotypic detection of multi drug resistant coagulase-positive spp. isolates from canine pyoderma.

Background: Dermatological infections in dogs are challenging to treat due to antibiotic resistance, which leads to longer recovery time and the need for stronger antibiotics. This study was undertaken to determine the prevalence of antimicrobial resistance in coagulase-positive staphylococcal isolates from pyoderma infection in dogs. This study also aimed to identify isolates with methicillin-resistance and multidrug resistance.

Milking reactivity influences daily yield and electrical resistance of milk in Jaffarabadi buffaloes.

Background: Buffalo reactivity during milking affects milking procedures, milk yield, and quality.

Aims: This study evaluated the influence of milking reactivity on the yield, composition, somatic cell count, pH, and electrical resistance of milk in Jaffarabadi buffaloes.

Methods: A 1-4 point scale, based on leg movement, was used to assess the milking reactivity of buffaloes (n=40).

The Effect of Different Concentrations of Melatonin on the Quality of Frozen and Thawed Semen of Jamnapari Goat.

Antioxidants help safeguard sperm cells from damage during the freeze-thaw process. Melatonin and its metabolites have an antioxidative effect. The current study aimed to evaluate the impact of melatonin supplementation in tris-based extenders at various concentrations (0.

Assessment of feeding hydroponic fodder on growth and reproduction performance in Black Bengal goat.

This study aimed to evaluate the effects of hydroponic fodder on the growth and reproductive performance of Black Bengal goats in East Champaran, Bihar, during the years 2020-21 and 2021-22. A total of 24 goats were randomly assigned to four groups: Control (C) with extensive grazing, gram straw, concentrate feed, and green fodder; Treatment 1 (T1) receiving C + 300 g/day of wheat hydroponic fodder; Treatment 2 (T2) with 300 g/day of maize hydroponic fodder alone; and Treatment 3 (T3) receiving C + a combination of 150 g/day of wheat and 150 g/day of maize hydroponic fodder. Each group comprised 6 animals.

Endogenous Tissue Inhibitor of Metalloproteinase-2 Levels Are Associated With High-Quality Neat Semen but Unrelated to Sperm Cryoresistance in Bulls.

Tissue Inhibitor of Metalloproteinase-2 (TIMP-2) is part of the tissue inhibitors of the metalloproteinases (TIMPs) family. Its primary function is to regulate the activity of matrix metalloproteinases (MMPs) across various tissues, including those of the reproductive system. This study aimed to quantify the natural levels of TIMP-2 in seminal plasma (SP) and sperm membrane (SM) of bulls, explore potential associations between TIMP-2 levels and semen quality parameters, and examine the relationship between TIMP-2 levels and sperm cryoresistance in bulls.

Deletion of both anaerobic regulator genes fnr and narL compromises the colonization of Salmonella Typhimurium in mice model.

Salmonella Typhimurium (STM), a zoonotic pathogen, can adjust its metabolic pathway according to the variations in the partial pressure of atmospheric oxygen and nitrate via fumarate nitrate reductase regulator (Fnr) and NarL, the response regulator for nitrate reductase. Both Fnr and NarL have been individually reported to be the contributors of virulent phenotypes of STM. Hypoxia along with nitrate-rich environment are prevalent in macrophages and the Salmonella-induced inflammatory lumen of the host's large intestine activates both fnr and narL genes.

Assessment of farmers' knowledge, attitudes and control practices (KAP) to mitigate acaricide resistance and tick-borne diseases.

A knowledge, attitudes and control practices (KAP)-based study on ticks and tick-borne diseases (TTBD) and resistance development in ticks was conducted in Dhar district of Madhya Pradesh covering 200 livestock owners using a questionnaire. Based on our scoring criteria, results indicated only 25% (19.16–31.

Exploring body morphometry and weight prediction in Ganjam goats in India through principal component analysis.

The body conformations of 262 adult Ganjam goats were subjected to principal component analysis (PCA) with 11 morphometric variables. The results were then used to predict the mature body weight of the goats. Most of the traits were positively correlated, and the correlations were statistically significant.

Applications of Artificial Intelligence for Heat Stress Management in Ruminant Livestock.

Heat stress impacts ruminant livestock production on varied levels in this alarming climate breakdown scenario. The drastic effects of the global climate change-associated heat stress in ruminant livestock demands constructive evaluation of animal performance bordering on effective monitoring systems. In this climate-smart digital age, adoption of advanced and developing Artificial Intelligence (AI) technologies is gaining traction for efficient heat stress management.

Isolation and Characterization of Extended-Spectrum β-Lactamase Producing from Pig Farms and Slaughterhouse.

Extended-spectrum β-lactamase (ESBL) producing represents a formidable challenge in the field of microbiology and public health due to its resistance to commonly used antibiotics. These strains pose a serious threat to human and animal health, underscoring the urgency of comprehensive research and surveillance. The ongoing investigation seeks ESBL producing strains from pig farms and slaughterhouses in West Bengal and Assam, India.

Antibiotic Resistance and Virulence Gene Patterns Associated with Multi Drug Resistant Avian Pathogenic (APEC) Isolated from Broiler Chickens in India.

Unlabelled: In the present study, a total of 102 samples were collected from chickens of different flocks, died due to suspected colibacillosis. Bacteriological and PCR methods were applied to detect avian pathogenic (APEC). Phenotypic antimicrobial resistance (AMR) was determined by disk diffusion method.

Active surveillance and associated epidemiological risk factors of caprine paratuberculosis in selected district of Odisha, India.

Caprine paratuberculosis (PTB) is a progressive, debilitating and production-limiting disease that causes significant economic losses and raises public health concerns. The goal was to study active surveillance and associated epidemiological risk factors of caprine PTB in selected district of Odisha, India. The 818 goats of various ages, sexes and breeds were randomly screened in ten different districts for a year based on history, clinical signs and fecal smear examination using the Ziehl-Neelsen stain, yielding an overall prevalence of 38.

Toxic effects of dibutyl phthalate on testes of adult zebrafish: evaluation of oxidative stress parameters and histopathology.

Dibutyl phthalate (DBP) is a phthalic compound and is most commonly used as a plasticizer in the polymer industry. It affects the hypothalamus-pituitary-gonadal axis and produces infertility in exposed animals. A total of 366 adult male zebrafish were used to evaluate the toxicological effects of DBP in testes following continuous exposure for 28 days.

CRISPR-based electrochemical biosensors for animal health: Recent advances.

Animal diseases are a major concern to animal welfare, human health and the global economy. Early detection, prevention and control of these animal diseases are crucial to ensure sustainability of livestock sector, to reduce farm losses and protecting public health. Points of care (POC) devices are small, portable instruments that provide rapid results thus reduce the risk of disease transmission and enable early intervention.

Geospatial Analysis of Lumpy Skin Disease Outbreaks among Cattle in Uttar Pradesh, India, 2021-2022.

The emergence of lumpy skin disease (LSD) among cattle in India is concerning. District-level data on LSD cases in Uttar Pradesh between 2021 and 2022 were analyzed. A stepwise spatial analytical approach was followed by first constructing yearly and monthly disease maps for LSD incidence rates (IRs), then spatially interpolating the LSD IRs, followed by evaluating the global and local clustering of LSD IRs and finally conducting spatial regression modeling.

The vasodilator effect of Eugenol on uterine artery - potential therapeutic applications in pregnancy-associated hypertension.

Preeclampsia, a gestational associated hypertension, has been reported in 6-8% of pregnant women worldwide leading to premature delivery and low birth weight of newborn due to reduced blood flow to placenta. Although several vasodilators (Methyl dopa, hydralazine, β-blockers and diuretics) are currently in use to treat preeclampsia, still there is a search for safer drugs with better efficacy. Lately, antihypertensive vasodilators from natural sources are gaining importance in treating preeclampsia.