Topping and grafting affect the alkaloid content and gene expression patterns of tobacco ( L.).

In this study, we aimed to determine molecular mechanisms underlying the effects of topping and grafting in tobacco ( L.) by comparing the alkaloid contents and enrichment pathways of differentially expressed genes (DEGs) among plants subjected to different combinations of topping and grafting treatments. Plants of the tobacco variety "Zhongyan 100" and eggplant ( L.