Genome-Wide Identification of ERF Transcription Factor Family and Functional Analysis of the Drought Stress-Responsive Genes in .

As an important forage legume with high values in feed and medicine, has been widely cultivated. The AP2/ERF transcription factor has been shown to play an important regulatory role in plant drought resistance, but it has not been reported in the legume forage crop . To digger the genes of in response to drought stress, we identified and analyzed the ERF gene family of at the genome-wide level.

Effect of temperature and water potential on the germination of seeds from three different populations of Bidens pilosa as a potential Cd hyperaccumulator.

Background: Bidens pilosa L., an annual herb, has recently been shown to be a potential Cd-hyperaccumulating plant. The germination characteristics of B.

Association between rumen microbiota and marbling grade in Hu sheep.

The marbling fat regulates the flavor of mutton and measures the fat density in the loin eye and is the most important parameter of carcass grading. The objective of this study was to explore the relationship of rumen microbiota and mutton marbling grade. One hundred and eighty-seven feedlot-finished Hu male lambs (Age: 180 day; Final BW: 46.

Full-length transcriptional analysis reveals the complex relationship of leaves and roots in responses to cold-drought combined stress in common vetch.

Plant responses to single or combined abiotic stresses between aboveground and underground parts are complex and require crosstalk signaling pathways. In this study, we explored the transcriptome data of common vetch ( L.) subjected to cold and drought stress between leaves and roots meta-analysis to identify the hub abiotic stress-responsive genes.

Microecosystem of yak rumen on the Qinghai-Tibetan Plateau is stable and is unaffected by soil or grass microbiota.

The rumen of livestock grazing on the Qinghai-Tibetan Plateau (QTP) acts as a transfer station for the circulation of soil, grass, faecal mineral elements and nutrients. Whether the microorganisms from the soil and grass could circulate through livestock rumen and excreted faeces. We studied the structural composition and interactive networks of microbiomes (bacteria and fungi) in soil, grass, and grazing yaks (rumen and faeces) on the QTP by using 16S rRNA gene and internally transcribed spacer (ITS) sequencing technology and to calculate the contribution rate of microorganisms from one habitat to another habitat using SourceTracker analysis.

Satellite greenness and solar-induced chlorophyll fluorescence reveal reverse desertification in Gurbantunggut Desert.

The desertification reversal is a process of revegetation and natural restoration in fragile dryland areas due to human activities and climate change mediation. Understanding the impact of desertification reversion on terrestrial ecosystems, including vegetation greenness and photosynthetic capacity, is crucial for land policy-making and carbon-cycle model improvement. However, the phenomenon of desertification reversal is rarely mentioned in previous studies, which dramatically limits the understanding of vegetation dynamics in the arid area.

Microbially enhanced methane uptake under warming enlarges ecosystem carbon sink in a Tibetan alpine grassland.

The alpine grasslands of the Tibetan Plateau store 23.2 Pg soil organic carbon, which becomes susceptible to microbial degradation with climate warming. However, accurate prediction of how the soil carbon stock changes under future climate warming is hampered by our limited understanding of belowground complex microbial communities.

Comparison of rumen bacterial communities between yaks () and Qaidam cattle () fed a low protein diet with different energy levels.

The yak (), an indigenous bovine on the Qinghai-Tibetan plateau (QTP), is reported to digest low quality forage to a greater extent and to require less protein and energy for maintenance than the introduced Qaidam cattle (). Ruminal bacteria play a major role in feed degradation, and therefore, we hypothesized that ruminal bacteria composition would differ between yaks and cattle, and confer an advantage to yaks for poor quality diets. To test our hypothesis, we determined the ruminal bacteria profiles, rumen fermentation parameters, and enzyme activities in these bovine species consuming a low-protein diet differing in energy level.

: A Bovid-Specific Y-Chromosome Multicopy Gene Is Highly Related to Postnatal Testicular Growth in Hu Sheep.

(preferentially expressed antigen in melanoma, Y-linked) belongs to the cancer-testis antigens (CTAs) gene family and is predominantly expressed in testis, playing important roles in spermatogenesis and testicular development. This study cloned the full-length cDNA sequence of ovine using the rapid amplification of cDNA ends (RACE) method and analyzed the expression profile and copy number variation (CNV) of using quantitative real-time PCR (qPCR). The results revealed that the cDNA was 2099 bp in length with an open reading frame (ORF) of 1536 bp encoding 511 amino acids.

Identification of Three Endophytes from Wilensky in China.

Cool season grasses often form reciprocal symbiotic relationships with endophytic fungal species in genus . In this study, we characterized three fungal endophytes isolated from the grass native to northwest China. Based on morphological characteristics and phylogenetic analyses of , , and sequences, we identified them as sp.

Evaluation of salt tolerance in common vetch (Vicia sativa L.) germplasms and the physiological responses to salt stress.

Common vetch (Vicia sativa L.) is an important leguminous crop, providing humans with starch from seeds, feeding livestock with vegetative organs, or fertilizing soils by returning to field. It is aimed to evaluate salt tolerance in common vetch collections for breeding programs and to investigate the underlined physiological mechanisms.

Root exudates and rhizosphere soil bacterial relationships of are linked to k-strategists bacterial community under salt stress.

When plants are subjected to various biotic and abiotic stresses, the root system responds actively by secreting different types and amounts of bioactive compounds, while affects the structure of rhizosphere soil bacterial community. Therefore, understanding plant-soil-microbial interactions, especially the strength of microbial interactions, mediated by root exudates is essential. A short-term experiment was conducted under drought and salt stress to investigate the interaction between root exudates and rhizosphere bacterial communities.

Ecological thresholds of toxic plants for sheep production and ecosystem multifunctionality and their trade-off in an alpine meadow.

Toxic plants are a natural component of alpine meadow which co-evolved with Tibetan sheep for thousands of years. One challenge for indigenous herders is to know the ecological thresholds of toxic plants and maintain their vital functions in ways that are compatible with economic income and ecological conservation. To achieve this, field trials with Tibetan sheep grazing in alpine meadow were conducted to examine the ecological thresholds of toxic plants for sheep production and ecosystem functions and their trade-offs.

Transcriptome analysis and identification of abscisic acid and gibberellin-related genes during seed development of alfalfa (Medicago sativa L.).

Background: Alfalfa (Medicago sativa) is a widely cultivated plant. Unlike many crops, the main goal of breeding alfalfa is to increase its aboveground biomass rather than the biomass of its seeds. However, the low yield of alfalfa seeds limits alfalfa production.

Probiotic effect of ferulic acid esterase-producing inoculated alfalfa silage on digestion, antioxidant, and immunity status of lactating dairy goats.

A feeding experiment was conducted to determine the effects of inoculating alfalfa silage with a ferulic acid esterase-producing inoculum on feed digestibility, rumen fermentation, antioxidant, and immunity status of lactating dairy goats. Twenty dairy goats were distributed into 2 experimental groups consisting of control diet (Lp MTD/1, including MTD/1 inoculated silage) against diet containing silage treated with ferulic acid esterase-producing A1 (Lp A1). Alfalfa silage inoculated with a ferulic acid esterase-producing Lp A1 had better fermentation quality than the Lp MTD/1 inoculation.

Ovine and gene polymorphisms and their association with tail fat deposition in Hu sheep.

Excessive fat deposition in the tail of sheep will affect its feed efficiency, which will increase the feeding cost. The purpose of this study was to identify the single nucleotide polymorphisms (SNPs) of and genes by PCR amplification and Sanger sequencing, the SNPs were genotyped by KASP genotyping assays to evaluate their association with tail fat deposition traits. The results showed that two intronic mutations of g.

Different dietary sources of selenium alter meat quality, shelf life, selenium deposition, and antioxidant status in Hu lambs.

Thirty-two male Hu lambs (32.31 ± 3.31 kg; 4-months-old) were randomly assigned to four treatments: (1) control (CON), (2) selenium-enriched yeast (SeY, 0.

Effect of Plateau Pika Disturbance on Plant Aboveground Biomass of Alpine Meadows at Two Different Scales.

Disturbance by small burrowing herbivores often has an impact on plant aboveground biomass of grassland because it makes grasslands into a mosaic of discrete vegetated surfaces and bare soil patches. This study focuses on the plateau pika () to investigate the effect of the disturbance by a small burrowing herbivore on plant aboveground biomass through upscaling the quadrat scale to the plot scale across five sites. This study showed that the plateau pika disturbance reduced sedge biomass and increased forb biomass.

Sheep fecal transplantation affects growth performance in mouse models by altering gut microbiota.

Animal growth traits are important and complex traits that determine the productivity of animal husbandry. There are many factors that affect growth traits, among which diet digestion is the key factor. In the process of animal digestion and absorption, the role of gastrointestinal microbes is essential.

Estimation of genetic correlations of two key feed efficiency traits with production traits in male Hu sheep.

Increased body growth and feed efficiency are important for breeding due to expensive feed costs. This study estimated the genetic parameters for two feed efficiency traits [the feed conversion ratio (FCR) and the residual feed intake (RFI)] and other important economic traits in male Hu sheep using 1642 male Hu lambs. The heritability of the RFI was estimated at 0.

Antifungal, Repellency, and Insecticidal Activities of Cymbopogon distans and Ruta graveolens Essential Oils and Their Main Chemical Constituents.

Essential oils produced by Cymbopogon distans and Ruta graveolens with a similar Chinese name could be explained as book fragrance in the Chinese idiom 'shu xiang men di', namely, a wealthy intellectual family according to ancient Chinese. Therefore, volatile oils from these two plants and their main compounds were tested to explore their antifungal, repellent, and insecticide actions. In this study, the essential olis (EO) of C.

Trace elements apportionment in forage, soil, and livestock in rangeland ecosystems along climatic gradients.

Background: Alpine meadows, typical steppes, and deserts are among the globally important rangeland types that are generally distributed along temperature and precipitation gradients. Mineral losses caused by grazing are one of the key factors that can lead to instability or even degradation of these rangeland ecosystems.

Methods: We examined the concentrations of Cu, Fe, Mn, and Zn in soil, forage, and livestock dungs from diverse rangeland types in northwest China, to determine the relationships between these trace elements (TEs) concentrations and climatic factors (i.

[Biological characteristics of heat shock transcription factors and their roles in abiotic stress adaptation of higher plant].

Heat shock transcription factors (HSFs) are involved in the regulation of plant growth and development. Furthermore, HSFs regulate the expression of a series of genes related to various abiotic stress adaptations. HSFs usually form homotrimers to activate their transcriptional activity and function.

The urgent need to develop a new grassland map in China: based on the consistency and accuracy of ten land cover products.

Grasslands are the most dominant terrestrial ecosystem in China, but few national grassland maps have been generated. The grassland resource map produced in the 1980s is widely used as background data, but it has not been updated for almost 40 years. Therefore, a reliable map depicting the current spatial distribution of grasslands across the country is urgently needed.