High Content Image Analysis of Cellular Responses of the Murine J774A.1 Cell Line and Primary Human Cells Alveolar Macrophages to an Extended Panel of Pharmaceutical Agents.

Introduction: In vitro screening of macrophages for drug-induced effects, such as phospholipidosis, is useful for detecting potentially problematic compounds in the preclinical development of oral inhaled products. High-content image analysis (HCIA) is a multi-parameter approach for cytotoxicity screening. This study provides new insights into HCIA-derived response patterns of murine J774A.

Covalent organic frameworks for radioactive iodine capture: structure and functionality.

The adsorption of radioactive iodine is a critical concern in nuclear safety and environmental protection due to its hazardous nature and long half-life. Covalent organic frameworks (COFs) have emerged as promising materials for capturing radioactive iodine owing to their tunable porosity, high surface area, and versatile functionalization capabilities. This review provides a comprehensive overview of the application of COFs in the adsorption of radioactive iodine.

Sarcocystis Cymruensis in the Brown Rat (Rattus Norvegicus) from an Urban District in Kuwait: Detailed Morphologic and Molecular Characterization.

Purpose: The objective of the study was to establish the prevalence of Sarcocystis (Apicomplexa, Sarcocystidae) in brown rats from Jleeb Al-Shuyoukh, Kuwait, and to describe detected parasites using morphological and DNA analysis methods.

Methods: Ninety-eight brown rats (Rattus norvegicus) were examined for Sarcocystis spp. Obtained sarcocysts were investigated using light microscopy and electron microscopy.

WISP1 inhibition of YAP phosphorylation drives breast cancer growth and chemoresistance via TEAD4 activation.

Wnt1-inducible signaling pathway protein 1 (WISP1) promotes breast cancer. The Hippo signaling pathway demonstrates a potential connection with WISP1, necessitating an exploration of their interaction. This study hypothesized that WISP1 boosts breast cancer by modulating the Hippo signaling pathway.

DPV pUL15 possesses a potential NLS, which is important for the location of the terminase complex and for viral proliferation and genome cleavage.

In herpesvirus, the terminase subunit pUL15 is involved in cleavage of the viral genome concatemers in the nucleus. Previous studies have shown that herpes simplex virus 1 (HSV-1) pUL15 can enter the nucleus without other viral proteins and help other terminase subunits enter the nucleus. However, this study revealed that duck plague virus (DPV) pUL15 cannot localize independently to the nucleus and can only be localized in the nucleus in the presence of pUL28 and pUL33.

Phylogenomic resolution of lampreys reveals the recent evolution of an ancient vertebrate lineage.

Jawless vertebrates once dominated Palaeozoic waters, but just two lineages have persisted to the present day: lampreys and hagfishes. Living lampreys are a relatively small clade, with just over 50 species described, but knowledge of their evolutionary relationships has always been based on either a few mitochondrial genes or a small number of taxa. Biogeographers have noted the disjunct antitropical distribution of living lamprey families.

Interaction Between the Matrix Protein and the Polymerase Complex of Respiratory Syncytial Virus.

The global burden of respiratory syncytial virus (RSV) and severe associated disease is prodigious. RSV-specific vaccines have been launched recently but there is no antiviral medicine commercially available. RSV polymerase (L) protein is one of the promising antiviral targets, along with fusion and nucleocapsid proteins.

On Security Performance of SWIPT Multi-User Jamming Based on Mixed RF/FSO Systems with Untrusted Relay.

This paper presents research on the security performance of a multi-user interference-based mixed RF/FSO system based on SWIPT untrusted relay. In this work, the RF and FSO channels experience Nakagami-m fading distribution and Málaga (M) turbulence, respectively. Multiple users transmit messages to the destination with the help of multiple cooperating relays, one of which may become an untrusted relay as an insider attacker.

, an / Family Gene, Involved in the Regulation of Seed-Specific Traits in Rice.

The Aux/IAA family proteins, key components of the auxin signaling pathway, are plant-specific transcription factors with important roles in regulating a wide range of plant growth and developmental events. The family genes have been extensively studied in Arabidopsis. However, most of the family genes in rice have not been functionally studied.

Comparison of Two Chelator Scaffolds as Basis for Cholecystokinin-2 Receptor Targeting Bimodal Imaging Probes.

: Dual-modality probes, combining positron emission tomography (PET) with fluorescence imaging (FI) capabilities in a single molecule, are of high relevance for the accurate staging and guided resection of tumours. We herein present a pair of candidates targeting the cholecystokinin-2 receptor (CCK2R), namely [Ga]Ga-CyTMG and [Ga]Ga-CyFMG. In these probes, the SulfoCy5.

Iodine-123 Metaiodobenzylguanidine (I-123 MIBG) in Clinical Applications: A Comprehensive Review.

Iodine-123 metaiodobenzylguanidine (I-123 MIBG) is a crucial radiopharmaceutical widely used in nuclear medicine for its diagnostic capabilities in both cardiology and oncology. This review aims to present a comprehensive evaluation of the clinical applications of I-123 MIBG, focusing on its use in diagnosing and managing various diseases. In cardiology, I-123 MIBG has proven invaluable in assessing cardiac sympathetic innervation, particularly in patients with heart failure, where it provides prognostic information that guides treatment strategies.

Hepatitis C Virus NS5A Activates Mitophagy Through Cargo Receptor and Phagophore Formation.

Chronic HCV infection is a risk factor for end-stage liver disease, leading to a major burden on public health. Mitophagy is a specific form of selective autophagy that eliminates mitochondria to maintain mitochondrial integrity. HCV NS5A is a multifunctional protein that regulates the HCV life cycle and may induce host mitophagy.

Experimental Study on the Mechanism of Enhanced Imbibition with Different Types of Surfactants in Low-Permeability Glutenite Reservoirs.

Due to the complex physical properties of low-permeability glutenite reservoirs, the oil recovery rate with conventional development is low. Surfactants are effective additives for enhanced oil recovery (EOR) due to their good ability of wettability alteration and interfacial tension (IFT) reduction, but the reason why imbibition efficiencies vary with different types of surfactants and the mechanism of enhanced imbibition in the glutenite reservoirs is not clear. In this study, the imbibition efficiency and recovery of surfactants including the nonionic, anionic, and cationic surfactants as well as nanofluids were evaluated and compared with produced water (PW) using low-permeability glutenite core samples from the Lower Urho Formation in the Mahu oil field.

Engineered Strategies for Lipid Droplets-Targeted AIEgens Based on Tetraphenylethene.

Lipid droplets (LDs), once regarded as inert fat particles, have been ignored by scientific researchers for a long time. Now, studies have shown that LDs are dynamic organelles used to store neutral lipids in cells and maintain cell stability. The abnormality of intracellular LDs usually causes metabolic disorders in the body, such as obesity, atherosclerosis, diabetes, and cancer, so the LDs have attracted wide attention.

Effect of Heat Treatment on the Microstructure and Property of Metastable β Titanium Alloy.

TB18 is a newly developed high-strength metastable β-titanium alloy, commonly used in aerospace structural materials, which demands high mechanical performance. By altering the alloy's microstructure, heat treatment can affect its mechanical characteristics. The alloy was solution treated for one to four hours at 870 °C in order to examine the impact of solution treatment duration.

Pharmacological Mechanisms of Bile Acids Targeting the Farnesoid X Receptor.

Bile acids (BAs), a category of amphiphilic metabolites synthesized by liver cells and released into the intestine via the bile duct, serve a vital role in the emulsification of ingested fats during the digestive process. Beyond their conventional emulsifying function, BAs, with their diverse structures, also act as significant hormones within the body. They are pivotal in facilitating nutrient absorption by interacting with the farnesoid X receptor (FXR), and they serve as key regulators of lipid and glucose metabolism, as well as immune system balance.

Sex and Age Differences in Glucocorticoid Signaling After an Aversive Experience in Mice.

Background: glucocorticoids may play an important role in the formation of fear memory, which is relevant to the neurobiology of post-traumatic stress disorder (PTSD). In our previous study, we showed the glucocorticoid receptor (GR) forms a protein complex with FKBP51, which prevents translocation of GR into the nucleus to affect gene expression; this complex is elevated in PTSD patients and by fear-conditioned learning in mice, and disrupting this complex blocks the storage and retrieval of fear-conditioned memories. The timing of release of glucocorticoid relative to the formation of a traumatic memory could be important in this process, and remains poorly understood.

RELT Is Upregulated in Breast Cancer and Induces Death in Breast Cancer Cells.

Background: Receptor Expressed in Lymphoid Tissues (RELT) is a TNFRSF member that has two paralogs, RELL1 and RELL2; the three proteins are collectively referred to as RELT family members (RELTfms).

Methods: We sought to evaluate RELT expression in cancerous cells by using real-time PCR, western blotting, flow cytometry, and immunohistochemistry (IHC). The mechanism of RELT-induced cell death was assessed by western blotting, flow cytometry, luciferase assays, and morphology staining.

Establishment of Local Diagnostic Reference Levels for Head CT Imaging in the Madina Region, Saudi Arabia.

: Computed Tomography (CT) is crucial in medical diagnosis, particularly for head examinations. Diagnostic Reference Levels (DRLs) are pivotal in balancing diagnostic efficacy with radiation safety. International organizations such as the International Atomic Energy Agency (IAEA) and the International Commission on Radiological Protection (ICRP) provide guidelines for establishing DRLs, emphasizing their importance in optimizing radiation doses.

Improvement of Sugar-Free Gummy Jellies' Properties by Phycocyanin Under Ultraviolet (UV) Irradiation.

In this study, (UP) was used as the primary research material, and sugar-free gummy jelly was prepared using ultraviolet (UV) irradiation with phycocyanin. The properties were measured using a texture analyzer, color difference analyzer, low-field nuclear magnetic resonance (LF-NMR) analyzer, and sensory evaluation. Additionally, the stability during accelerated storage was examined.

First Description of the Role of the Relationship Between Serum Amyloid P Components and Nuclear Factors/Pro-Cytokines During Critical Periods of Toxoplasmic Encephalitis.

(), an obligate food-borne intracellular parasite, causes severe neuropathology by establishing a persistent infection in the host brain. We have previously shown that infection induces severe neuropathology in the brain manifested by increased nitric oxide production, oxidative stress, glial activation/BBB damage, increased pro-inflammatory cytokine glia maturation factor-beta and induced apoptosis. The aim of this experimental study was to investigate the serum amyloid P (SAP) components, nuclear factor kappa B (NF-κB), interleukin-1 beta (IL-1β), caspase 1 (Casp 1), tumor necrosis factor-alpha (TNF-α) and complement 3 (C3) gene expressions on the 10th, 20th and 30th days after infection with in the neuroimmunopathogenesis of toxoplasmic encephalitis (TE) in mouse brains by real-time quantitative polymerase chain reaction.

The Physiological Functions and Therapeutic Potential of Hypoxia-Inducible Factor-1α in Vascular Calcification.

HIF-1α plays a crucial regulatory role in vascular calcification (VC), primarily influencing the osteogenic differentiation of VSMCs through oxygen-sensing mechanisms. Under hypoxic conditions, the stability of HIF-1α increases, avoiding PHD and VHL protein-mediated degradation, which promotes its accumulation in cells and then activates gene expressions related to calcification. Additionally, HIF-1α modulates the metabolic state of VSMCs by regulating the pathways that govern the switch between glycolysis and oxidative phosphorylation, thereby further advancing the calcification process.

Vanillin Has Potent Antibacterial, Antioxidant, and Anti-Inflammatory Activities In Vitro and in Mouse Colitis Induced by Multidrug-Resistant .

A large number of cases of infectious colitis caused by multidrug-resistant (MDR) bacteria, such as , can result in colon damage and severe inflammation. Vanilla, a widely utilized flavor and fragrance compound, is extensively used in various food. However, the effect of vanilla on MDR -induced infectious colitis has received less attention.

Erinacine A-Enriched Mycelium Ethanol Extract Lessens Cellular Damage in Cell and Models of Spinocerebellar Ataxia Type 3 by Improvement of Nrf2 Activation.

Spinocerebellar ataxia type 3 (SCA3), caused by the abnormal expansion of polyglutamine (polyQ) in the ataxin-3 protein, is one of the inherited polyQ neurodegenerative diseases that share similar genetic and molecular features. Mutant polyQ-expanded ataxin-3 protein is prone to aggregation in affected neurons and is predominantly degraded by autophagy, which is beneficial for neurodegenerative disease treatment. Not only does mutant polyQ-expanded ataxin-3 increase susceptibility to oxidative cytotoxicity, but it also hampers antioxidant potency in neuronal cells.