E3 ligase MIB1 regulates STAT1/P21 signaling via regulation of FGFR1 in colorectal cancer.

Background: Mind bomb 1 (MIB1) is an E3 ubiquitin ligase that promotes the polyubiquitination-mediated degradation of NOTCH ligands and plays an important role in various cancers by enhancing tumor cell proliferation. Also, MIB1 inhibited the cell cycle progression by transcriptional repression of P21 in HCT116 cells. Fibroblast growth factor receptor 1 (FGFR1) is a receptor tyrosine kinase (RTK) that plays a significant role in the progression of various cancers.

Robust phylogenetic tree-based microbiome association test using repeatedly measured data for composition bias.

Background: The effects of microbiota on the host phenotypes can differ substantially depending on their age. Longitudinally measured microbiome data allow for the detection of the age modification effect and are useful for the detection of microorganisms related to the progression of disease whose identification change over time. Moreover, longitudinal analysis facilitates the estimation of the within-subject covariate effect, is robust to the between-subject confounders, and provides better evidence for the causal relationship than cross-sectional studies.

Design, Synthesis, and Biological Activity of Novel Ornithine Decarboxylase (ODC) Inhibitors.

We here describe the design, synthesis, and biological activity of novel ornithine decarboxylase (ODC) inhibitors that show significantly higher potency than α-difluoromethylornithine (DFMO), a U.S. Food and Drug Administration (FDA) approved drug.

Inflamed Tissue-Targeting Polyphenol-Condensed Antioxidant Nanoparticles with Therapeutic Potential.

Inflammation is essential for pathogen eradication and tissue repair; However, chronic inflammation can bring on multi-organ dysfunction due to an overproduction of reactive oxygen species (ROS). Among various anti-inflammatory agents, polyphenol-based nanotherapeutics offer potential advantages, including enhanced stability, targeted delivery, multiple therapeutic functions, and personalized therapy tailored to the severity. Despite these advantages, the development of biocompatible nanomedicines capable of selective accumulation in inflamed tissues and efficient inhibition of ROS-induced inflammatory signaling pathways remains a considerable challenge.

AmyA contributes to the glycogen synthesis in Sulfolobus acidocaldarius.

Glycogen, an α-1,4 linked glucose polymer with α-1,6 linked branches, accumulates in Sulfolobus acidocaldarius in granular form and contributes to stress resistance. While the glg operon responsible for glycogen metabolism has been studied, the gene responsible for branch formation remained elusive. Interestingly, the ΔAmyA mutant failed to accumulate glycogen.

Unlocking the genetic potential of Ethiopian fenugreek ( L.) genotypes for future breeding.

Fenugreek L.) is an annual spice crop and cultivated throughout the world for it's multipurpose. Even though it has long history of cultivation and importance of fenugreek, the knowledge on its variability is limited.

sp. nov., isolated from the rhizosphere of rice and genome mining for the prediction of biosynthetic gene clusters.

A Gram-stain-negative, aerobic, rod-shaped, motile and flagellated novel bacterial strain, designated MAHUQ-64, was isolated from the rhizosphere of rice. The colonies were observed to be creamy white-coloured, smooth, spherical and 0.5-1.

Atlantification advances into the Amerasian Basin of the Arctic Ocean.

Atlantification-the northward inflow of anomalous waters and biota from the Atlantic into the polar basins-has wide-ranging climatological ramifications. We present previously unknown observational evidence that the atlantification processes are strengthening in the eastern Eurasian Basin. The primary example is the diminishing sea ice, which is related to a powerful ocean-heat/ice-albedo feedback, which accelerates sea-ice losses.

Emodin, a Potent Anthraquinone Mitigates MPTP-Induced Parkinsons' Disease Pathology by Regulating Nrf2 and Its Downstream Targets: In Silico and In Vivo Approach.

Parkinson's disease (PD) is marked by neurodegeneration that follows the destruction of dopaminergic neurons, mainly localized to the substantia nigra. It results in debilitating motor as well as non-motor symptoms. The current study investigated the neuroprotective potential of emodin, a naturally occurring anthraquinone derivative, in a well-established model of PD in mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).

Autophagy Induction by Mangiferin Protects Auditory Hair Cells from Ototoxicity.

Oxidative stress is a major cause of auditory hair cell degeneration and hearing impairment.Reducing intracellular reactive oxygen species (ROS) levels may help preserve auditory hair cell function. In this study, we explored the otoprotective properties of mangiferin, a xanthonoid extracted from mango leaves, bark, and fruit peels.

Molecular Clustering of Metabolic Dysfunction-Associated Steatotic Liver Disease Based on Transcriptome Analysis.

Metabolic dysfunction-associated steatotic liver disease (MASLD) is a complex metabolic disorder with a diverse spectrum. This study aimed to classify patients with MASLD into molecular subtypes based on the underlying pathophysiology. We performed high-throughput RNA sequencing on 164 liver tissue samples from healthy controls and patients with MASLD.

Pregnane derivatives in wheat (Triticum aestivum) and their potential role in generative development.

Pregnane derivatives such as pregnenolone or progesterone and many other metabolites are important in mammals where many of them act as hormones including sexual hormones. Much less is known about the presence and functions of pregnane derivatives in plants. The main objectives of this work were (1) to determine the presence of pregnane derivatives in winter wheat (2) verify if there are changes of concentration of pregnane derivatives during wheat growth/development with special attention to vernalisation process (3) to answer the question of whether selected pregnane derivatives are stimulators of wheat development and whether the potential stimulation of this development is accompanied by the expression of the Vrn1 (Vernalisation1) gene.

Insights into the nuclear-organelle DNA integration in Cicuta virosa (Apiaceae) provided by complete plastid and mitochondrial genomes.

Background: Gene transfer between the organelles and the nucleus plays a central role in shaping plant genome evolution. The identification and analysis of nuclear DNA of plastid (NUPTs) and mitochondrial (NUMTs) origins are important for exploring the extent of intracellular DNA transfer in genomes.

Results: We report the complete plastid and mitochondrial genomes (plastome and mitogenome) of Cicuta virosa (Apiaceae) as well as a draft nuclear genome using high-fidelity (HiFi) PacBio sequencing technologies.

Mangiferin protects mesenchymal stem cells against DNA damage and cellular aging via SIRT1 activation.

The protective effects of mangiferin (MAG) against etoposide- and high glucose (HG)-induced DNA damage and aging were investigated in human bone marrow-mesenchymal stem cells (hBM-MSCs). Etoposide, a topoisomerase II inhibitor, was used to induce double-strand breaks (DSBs) in hBM-MSCs, resulting in increased genotoxicity, elevated levels of the DNA damage sensor ATM and CDKN1A, and decreased levels of the aging markers H3 and H4. MAG activated AMPK and SIRT1, thus protecting against DSB-induced damage.

PrP Glycoprotein Is Indispensable for Maintenance of Skeletal Muscle Homeostasis During Aging.

Background: The cellular prion protein (PrP), a glycoprotein encoded by the PRNP gene, is known to modulate muscle mass and exercise capacity. However, the role of PrP in the maintenance and regeneration of skeletal muscle during ageing remains unclear.

Methods: This study investigated the change in PrP expression during muscle formation using C2C12 cells and evaluated muscle function in Prnp wild-type (WT) and knock-out (KO) mice at different ages (1, 9 and 15 months).

UV-C and Nanomaterial-Based Approaches for Sulfite-Free Wine Preservation: Effects on Polyphenol Profile and Microbiological Quality.

Controlling the microorganisms employed in vinification is a critical factor for successful wine production. Novel methods aimed at lowering sulfites used for wine stabilization are sought. UV-C irradiation has been proposed as an alternative for reducing the viable cell count of microorganisms in wine and grape juice.

Assessment of radioactivity in soil samples from Wolaita Sodo town, Ethiopia: implications for environmental and public health.

This study assesses the activity concentrations of the radionuclides 238U, 232Th, and 40K in soil samples collected from Wolaita Sodo town, located in the Southern Nations, Nationalities, and Peoples' (SNNP) Region, Ethiopia. A gamma-ray spectrometer equipped with a NaI(Tl) detector was used for the measurements. The concentrations of 238U, 232Th, and 40K varied from 3.

Health and Economic Impact Estimation of Ambient Air Particulate Matter (PM) Pollution in Addis Ababa Using BenMAP-CE Model.

Exposure to ambient air particulate matter (PM) pollution presents a significant public health and economic challenge in Addis Ababa, Ethiopia. This thesis used the Environmental benefits mapping and analysis program-community edition (BenMAP-CE) software tool to estimate health and economic impact of ambient air PM pollution. The study evaluated the impact of decreasing the annual average PM concentration in 2019 (32.

Experiences of People With Type 1 Diabetes Using the iLet Bionic Pancreas in Primary Care: A Qualitative Analysis.

This qualitative substudy sought to identify and understand the experiences of primary care patients with type 1 diabetes using the iLet Bionic Pancreas (Beta Bionics, Inc.) during the first clinical trial testing the automated insulin delivery (AID) system in the primary care setting. Participants in that clinical trial completed a brief semi-structured interview after the trial's completion, during which they were asked questions related to their perceptions of and experiences with using the iLet AID system.

Reduced UPF1 levels in senescence impair nonsense-mediated mRNA decay.

Cells regulate gene expression through various RNA regulatory mechanisms, and this regulation often becomes less efficient with age, contributing to accelerated aging and various age-related diseases. Nonsense-mediated mRNA decay (NMD), a well-characterized RNA surveillance mechanism, degrades aberrant mRNAs with premature termination codons (PTCs) to prevent the synthesis of truncated proteins. While the role of NMD in cancer and developmental and genetic diseases is well documented, its implications in human aging remain largely unexplored.

Identifying Disease Associated Multi-Omics Network With Mixed Graphical Models Based on Markov Random Field Model.

In this article, we proposed a new method named fused mixed graphical model (FMGM), which can infer network structures associated with dichotomous phenotypes. FMGM is based on a pairwise Markov random field model, and statistical analyses including the proposed method were conducted to find biological markers and underlying network structures of the atopic dermatitis (AD) from multiomics data of 6-month-old infants. The performance of FMGM was evaluated with simulations by using synthetic datasets of power-law networks, showing that FMGM had superior performance for identifying the differences of the networks compared to the separate inference with the previous method, causalMGM (F1-scores 0.

Ovarian tumor microenvironment contributes to tumor progression and chemoresistance.

Ovarian cancer is one of the deadliest gynecologic cancers affecting the female reproductive tract. This is largely attributed to frequent recurrence and development of resistance to the platinum-based drugs cisplatin and carboplatin. One of the major contributing factors to increased cancer progression and resistance to chemotherapy is the tumor microenvironment (TME).

Ethnobotany of vascular plants use, conservation and management practice in the homegardens by the people of Dawuro in Southwestern Ethiopia.

Background: Homegardens (HGs) are well-time-honored traditional land use systems in small plots of land with purposely designed intricate structure and a mixture of planted vascular plants (VPs) for different purposes. Hence, the present study was initiated to investigate the ethnobotanical information of vascular plants of homegardens and their use, conservation and management practice by the people of Dawuro in southwestern Ethiopia.

Methods: A total of 162 farmer informants were selected and interviewed within a distance of < 2 km, 2-4 km and > 4 km between the natural forest and homegardens, and 0.

The effects of weight- and non-weight-bearing exercise on corticospinal axon sprouting, regeneration-related proteins and functional recovery after spinal cord contusion.

The purpose of this study was to investigate the effects of weight- and non-weight-bearing exercises on the Basso-Beattie-Bresnahan (BBB) locomotor rating scale, corticospinal axon regrowth and regeneration-related proteins following spinal cord injury (SCI). Twenty-four male Sprague-Dawley rats were randomly divided into four groups: control group (n=6), SCI+sedentary group (SED, n=6), SCI+treadmill exercise group (TREAD, n=6), and SCI+swimming exercise group (SWIM, n=6). All rats in the SCI group were given the rest for 2 weeks after SCI, and then they were allowed to engage in low-intensity exercise for 6 weeks on treadmill device.