OsWRKY26 negatively regulates bacterial blight resistance by suppressing expression.

Plants are susceptible to infection by various pathogens with high epidemic potential. pv () causes bacterial blight in rice, one of the most significant diseases in both temperate and tropical regions. In this study, we report the identification and characterization of , a sucrose-inducible transcription factor, that plays a role in the plant defense responses following infection.

Microscale Spatiotemporal Variation of Reactive Oxygen Species in the Charosphere: Underlying Formation Mechanism and Their Role in CO Emission.

Charosphere, a highly active zone between biochar and surrounding soil, is widely present in agricultural and wildfire-affected soils, yet whether reactive oxygen species (ROS) are produced within the charosphere remains unclear. Herein, the production and spatiotemporal evolution of charosphere ROS were explored. In situ ROS capture visualized a gradual decrease in ROS production with increasing distance from the biochar/soil interface.

Predictors associated with time to default for HIV/AIDS patients under HAART at Debre Tabor Referral Hospital: a Cox regression model.

The human immunodeficiency virus systematically undermines the immune system, which serves as our body's inherent safeguard against diseases. Currently, it is the most serious threat to public health. Ethiopia is among the countries with the highest prevalence of HIV/AIDS.

Assessment of radioactivity in soil samples from Wolaita Sodo town, Ethiopia: implications for environmental and public health.

This study assesses the activity concentrations of the radionuclides 238U, 232Th, and 40K in soil samples collected from Wolaita Sodo town, located in the Southern Nations, Nationalities, and Peoples' (SNNP) Region, Ethiopia. A gamma-ray spectrometer equipped with a NaI(Tl) detector was used for the measurements. The concentrations of 238U, 232Th, and 40K varied from 3.

Amphiphilic Polyaspartamide Derivatives with Cholesterol Introduction Enhanced Ex Vivo mRNA Transfection Efficiency to Natural Killer Cells.

Engineered natural killer (NK) cells eliminate cancer cells by overexpressing a chimeric antigen receptor, producing highly efficient and safe NK cell therapies. This study investigated the polyplex formulation for the fusion protein GreenLantern-natural killer group 2D (NKG2D) mRNA to evaluate its delivery efficacy into NK cells, wherein NKG2D on the surface of NK cells recognized its counterpart NKG2D ligands on cancer cells. Amphiphilic polyaspartamide derivatives Chol-PAsp(DET/CHE) were prepared by adding cyclohexylethylamine (CHE) and diethylenetriamine (DET) in the side chains and cholesterol (Chol) at the α-terminus to enhance endosomal escapability and optimize hydrophobicity.

Growth and Yield Performance of Cultivated on Agricultural Residues.

Food insecurity and malnutrition are among the major problems in most developing nations recently. Mushroom cultivation is one of the promising strategies to overcome these challenges. The growth and productivity of mushrooms differ because of their wide range of cultivation substrates.

A Phosphate-Starvation Enhanced Purple Acid Phosphatase, GmPAP23 Mediates Intracellular Phosphorus Recycling and Yield in Soybean.

Plant internal phosphorus (P) recycling is a complex process, which is vital for improving plant P use efficiency. However, the mechanisms underlying phosphate (Pi) release from internal organic-P form remains to be deciphered in crops. Here, we functionally characterised a Pi-starvation responsive purple acid phosphatase (PAP), GmPAP23 in soybean (Glycine max).

Time to first antenatal care visit and its predictors among women in Kenya: Weibull gamma shared frailty model (based on the recent 2022 KDHS data).

Background: The first trimester of pregnancy is critical for fetal development, making early antenatal care visits essential for timely check-ups and managing potential complications. However, delayed antenatal care initiation remains a public health challenge in sub-Saharan Africa, including Kenya. Therefore, this study aimed to assess and provide up-to-date information on time to first antenatal care visit and its predictors among women in Kenya, using data from the most recent 2022 Kenya Demographic and Health Survey (KDHS).

Genomics yields biological and phenotypic insights into bipolar disorder.

Bipolar disorder is a leading contributor to the global burden of disease. Despite high heritability (60-80%), the majority of the underlying genetic determinants remain unknown. We analysed data from participants of European, East Asian, African American and Latino ancestries (n = 158,036 cases with bipolar disorder, 2.

A periplasmic protein modulates the proteolysis of peptidoglycan hydrolases to maintain cell wall homeostasis in .

Bacterial cell wall assembly and remodeling require activities of peptidoglycan (PG) hydrolases as well as PG synthases. In particular, the activity of DD-endopeptidases, which cleave the 4-3 peptide crosslinks in PG, is essential for PG expansion in gram-negative bacteria. Maintaining optimal levels of DD-endopeptidases is critical for expanding PG without compromising its integrity.

Indirubin-3'-oxime as a dual-action agent: mitigating heat-induced male infertility in and inhibiting soluble epoxide hydrolase.

This study investigated the potential of the indirubin-3'-oxime (I3O) compound to mitigate temperature-induced male infertility in . Elevated temperatures significantly reduced egg-hatching rates, but I3O supplementation improved these rates, suggesting it can partially restore fertility under heat stress. Additionally, I3O was found to inhibit soluble epoxide hydrolase (sEH), an enzyme involved in the metabolism of epoxyeicosatrienoic acids, which are vital for reproductive health.

Patient Experience Regarding Medication-Related Information Received in a Rural Private Polyclinic Setting in Vietnam.

Inadequate provision of medication-related information can lead to nonadherence to treatment. This study aims to investigate patient satisfaction regarding medication-related information provided by healthcare professionals and to identify factors associated with patient experience regarding information. A cross-sectional descriptive study was conducted in October 2023 among 400 patients at a rural private polyclinic in Ben Tre, Vietnam by using a self-administered questionnaire.

Health and Economic Impact Estimation of Ambient Air Particulate Matter (PM) Pollution in Addis Ababa Using BenMAP-CE Model.

Exposure to ambient air particulate matter (PM) pollution presents a significant public health and economic challenge in Addis Ababa, Ethiopia. This thesis used the Environmental benefits mapping and analysis program-community edition (BenMAP-CE) software tool to estimate health and economic impact of ambient air PM pollution. The study evaluated the impact of decreasing the annual average PM concentration in 2019 (32.

Quantifying Tinnitus Perception Improvement: Deriving the Minimal Clinically Important Difference of the Minimum Masking Level.

Purpose: Tools that can reliably measure changes in the perception of tinnitus following interventions are lacking. The minimum masking level, defined as the lowest level at which tinnitus is completely masked, is a candidate for quantifying changes in tinnitus perception. In this study, we aimed to determine minimal clinically important differences for minimum masking level.

Generalized linear modeling of HCV infection among medical waste handlers in Sidama region, Ethiopia.

Background: There is limited evidence on prevalence and risk factors for hepatitis C virus (HCV) infection among waste handlers in Sidama region, Ethiopia; however, this knowledge is necessary for effective prevention of HCV infection in the region.

Methods: A cross-sectional study was conducted among randomly selected waste collectors from October 2021 to 30 July 2022 in different public hospitals of Sidama region of Ethiopia. Serum samples were collected from participants and screened for anti-HCV using rapid immunochromatography assay.

Comparative performance of microscopy, rapid diagnostic tests, and multiplex real-time PCR for detection of malaria parasites among pregnant women in northwest Ethiopia.

Background: Low malaria parasitaemia is a diagnostic challenge in pregnancy, leading to false negative microscopy and rapid diagnostic test (RDT) results. However, these submicroscopic or subpatent infections could cause adverse pregnancy outcomes. Thus, evaluating the diagnostic performance of microscopy, RDT, and multiplex qPCR in pregnancy is vital for informed decisions.

APPLICATIONS OF MATHEMATICAL PROGRAMMING TO GENETIC BIOCONTROL.

We review existing approaches to optimizing the deployment of genetic biocontrol technologies-tools used to prevent vector-borne diseases such as malaria and dengue-and formulate a mathematical program that enables the incorporation of crucial ecological and logistical details. The model is comprised of equality constraints grounded in discretized dynamic population equations, inequality constraints representative of operational limitations including resource restrictions, and an objective function that jointly minimizes the count of competent mosquito vectors and the number of transgenic organisms released to mitigate them over a specified time period. We explore how nonlinear programming (NLP) and mixed integer nonlinear programming (MINLP) can advance the state of the art in designing the operational implementation of three distinct transgenic public health interventions, two of which are presently in active use around the world.

Experiences of People With Type 1 Diabetes Using the iLet Bionic Pancreas in Primary Care: A Qualitative Analysis.

This qualitative substudy sought to identify and understand the experiences of primary care patients with type 1 diabetes using the iLet Bionic Pancreas (Beta Bionics, Inc.) during the first clinical trial testing the automated insulin delivery (AID) system in the primary care setting. Participants in that clinical trial completed a brief semi-structured interview after the trial's completion, during which they were asked questions related to their perceptions of and experiences with using the iLet AID system.

Bispecific antibodies and CLEM: an analytical approach to advanced cell imaging for therapeutic strategies.

The development of bispecific antibodies (BsAbs) represents a significant advancement in therapeutic antibody design, enabling the simultaneous targeting of two different antigens. This dual-targeting capability enhances therapeutic efficacy, particularly in complex diseases like cancer, where tumor heterogeneity presents a significant challenge for traditional treatments. By bridging two distinct pathways, BsAbs can improve specificity and minimize off-target effects, making them invaluable in therapeutic contexts.

Cancer prognosis using base excision repair genes.

The base excision repair (BER) pathway is a critical mechanism in genomic stability. This review investigates the role of the BER pathway in advanced cancer therapies considering the pivotal role of genetic factors in cancer patient responses and prognosis. BER factors significantly influence genetic instability and cancer prognosis, as well as the effectiveness of chemotherapy and radiation therapy.

Novel green synthesis of Al-Fe₃O₄ nanocomposite for magnetic d-μSPE of Cd(II) from water and food samples.

A novel and green nanocomposite (Al-Fe₃O₄) was synthesized and used for the magnetic d-μSPE method for separating and enriching Cd(II) from dried fruit samples. Aluminum foil waste and banana peels were used as the precursors. The green nanocomposite was characterized using FTIR, XRD, and FE-SEM techniques.

Comparative analysis of floristic richness and diversity in six central forest reserves of north eastern Uganda.

As the extinction risk of plants increases globally, there is need to prioritize areas with high floristic richness and diversity to inform the design of evidence-based conservation interventions. As such, this study aimed to comparatively analyse floristic diversity in six central forest reserves (CFR) of north eastern Uganda. This was guided by two objectives namely; (i) to determine the floristic richness and diversity in the CFRs and (ii) to evaluate the similarity and complementarity of floristic composition.

Reduced UPF1 levels in senescence impair nonsense-mediated mRNA decay.

Cells regulate gene expression through various RNA regulatory mechanisms, and this regulation often becomes less efficient with age, contributing to accelerated aging and various age-related diseases. Nonsense-mediated mRNA decay (NMD), a well-characterized RNA surveillance mechanism, degrades aberrant mRNAs with premature termination codons (PTCs) to prevent the synthesis of truncated proteins. While the role of NMD in cancer and developmental and genetic diseases is well documented, its implications in human aging remain largely unexplored.

Seroprevalence of Hepatitis B and C and HIV Infections and Associated Risk Factors Among Pregnant Women Attending Antenatal Care Unit at Simada Hospital, South Gondar Zone, Northwest Ethiopia.

Hepatitis and human immunodeficiency virus (HIV) are major public health issues in developing countries, including Ethiopia. These viruses can be transmitted from mother to child during birth or through contact with contaminated blood. In many areas of Ethiopia, viral hepatitis and HIV infections are significant health concerns for pregnant women.