Identification of the matrix metalloproteinase (MMP) gene family of Trachinotus ovatus and expression analysis in response to Streptococcus agalactiae and Cryptocaryon irritans.

Matrix metalloproteinases (MMPs) represent a group of zinc-dependent endopeptidases that degrade various protein components of the extracellular matrix (ECM). These enzymes play a role in multiple biological and pathological processes, including tissue remodeling, growth, trauma repair, defense mechanisms, and immune response. However, their role in immune response in Trachinotus ovatus is still unclear.

Atmospheric chemistry of the coastal area is influenced by the convergence between the inland and marine air: Insight into carbonyl compounds.

Marine biological activity has long been recognized to impact the atmospheric chemistry of coastal areas. In this work, we monitored the seasonal variation of carbonyl compounds in the coastal city of Qingdao, located in the north of China's coastline and the south of Jiaodong Peninsula, with the vast hinterland in the west. The mean total concentration of the 15 carbonyls varied significantly between seasons, with the highest observed in autumn (10.

Indispensable role of PGC1α signaling in lipid and carbohydrate metabolism of fish PPARα activation.

Unlike predictability in mammals, the responses of PPARα activation treatment in fish varies between different fish species. Existing results indicated that the varied effects may result from fish feeding habits difference within their varied dietary protein content, while the exact mechanism remains obscure. In this study, we conducted an in vivo 8-week trial of low protein 30 % (LP) or high protein 38 % (HP) diet fed tilapia (8.

Structural evaluation and formation regularities of garlic clove-like meat in prefabricated grass carp.

Moderately marinated fish meat forms a garlic clove-like structure after cooking. However, this structure's formation mechanism and influencing factors are unclear. Therefore, this study explored the changes in protein components and potential protein markers during the formation of garlic clove-like structure.

How Fish Intestinal Cells Responded to Dietary Methylmercury Exposure? A Single-Cell Transcriptomic Analysis.

Fish intestine is not only an important digestive and immune organ, but also serves as the first barrier to defend against methylmercury (MeHg) toxicity. Numerous studies have examined the responses of intestine to MeHg, whereas the heterogeneous responses of intestinal cells have not been addressed. In this study, the gilthead seabream were exposed to dietary MeHg, and the gene expression profiles of different intestinal cell populations were examined using scRNA-seq technique.

Divergent responses of abundant and rare bacterial communities to environmental variables in highly urbanized coastal regions: N-NO mediates the community assembly and co-occurrence networks.

Understanding how rare and abundant bacterial taxa respond to environmental shifts is essential for predicting microbial community dynamics in highly urbanized estuarine ecosystems, where anthropogenic disturbances and nutrient fluctuations significantly reshape ecological processes. Here, we investigated the responses of rare and abundant bacterial communities to the environments in densely urbanized coastal areas (i.e.

A noncanonical intrinsic terminator in the HicAB toxin-antitoxin operon promotes the transmission of conjugative antibiotic resistance plasmids.

Conjugative plasmids, major vehicles for the spread of antibiotic resistance genes, often contain multiple toxin-antitoxin (TA) systems. However, the physiological functions of TA systems remain obscure. By studying two TA families commonly found on colistin-resistant IncI2 mcr-1-bearing plasmids, we discovered that the HicAB TA, rather than the StbDE TA, acts as a crucial addiction module to increase horizontal plasmid-plasmid competition.

EasyMetagenome: A user-friendly and flexible pipeline for shotgun metagenomic analysis in microbiome research.

Shotgun metagenomics has become a pivotal technology in microbiome research, enabling in-depth analysis of microbial communities at both the high-resolution taxonomic and functional levels. This approach provides valuable insights of microbial diversity, interactions, and their roles in health and disease. However, the complexity of data processing and the need for reproducibility pose significant challenges to researchers.

Clamworm bioturbation reduces mercury methylation through alteration of methylator composition in sediment.

Coastal sediment has been recognized as a hotspot of mercury (Hg) methylation and acts as an important reservoir for Hg-methylating microbes. The bioturbation behaviors of benthic organisms can significantly influence sediment properties and potentially affect the mobility and availability of contaminants within the sediment. However, the effects of bioturbation on Hg speciation and disposition in sediment have not been well addressed.

Decoding the aroma landscape of fermented golden pompano: The interplay of ester compounds and symbiotic microbiota as revealed by metagenomics and two-dimensional flavoromics.

Fermented pompano (Trachinotus ovatus) is a traditionally popular fermented seafood throughout Asia. Its distinctive flavor profile is primarily attributed to the microbial metabolic conversion of nutrients, which produces specific volatile compounds. Two-dimensional flavoromics of mature pompano revealed that various volatile flavor compounds accumulate throughout fermentation, with fruity (predominantly esters) and oleogustus (primarily ketones) being key flavor markers.

Fucoidan as an Encapsulant material: Applications and advantages in active agents and probiotic encapsulations.

Fucoidans are branched sulfated polysaccharides primarily derived from brown seaweed, especially the Fucus genus, as well as other algae and marine invertebrates. With pseudoplastic behavior, they offer promising applications in cosmetics, pHarmaceuticals, and food industries. While traditional drug delivery materials are well-studied, fucoidans have gained attention as encapsulants, particularly in combination with chitosan and whey protein concentrate, which enhances their biocompatibility, biodegradability, and bioactivity.

A portable biosensor based on exonuclease III-assisted amplification strategy for on-site monitoring and early warning of Phaeocystis globosa bloom.

In recent years, the frequent outbreaks of Phaeocystis globosa (P. globosa) bloom have seriously threatened the safety of coastal nuclear power. However, existing detection technologies cannot meet the demand for the early warning of P.

Evidence of the occurrence, detection, and ecotoxicity studies of perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA) in aqueous environments.

Perflorochemicals (PFCs), among which are the most commonly detected perfluorooctanesulfonic acid (PFOS) and perfluorooctanoic acid (PFOA), are persistent emergent contaminants of concern in recent times. These compounds have been reported for their cytotoxicity, genotoxicity, carcinogenicity, immunotoxicity, and developmental toxicities. Meanwhile, they have been detected in diverse matrices such as soil, sediment, and, surprisingly, in serum and even breastmilk.

Spatial Variation in Food Web Structures, Energy Flows, and System Attributes Along the Pearl River and Their Indications of Protection and Restoration.

River ecosystems are facing significant degradation from human activities, which impact both biotic (e.g., fish and invertebrates) and abiotic components (e.

The Effects of Climate Change on Habitats in the Northern Indian Ocean.

The northern Indian Ocean is located in a typical monsoon region that is also influenced by climate events such as the Indian Ocean Dipole (IOD), which makes habitat highly susceptible to changes in climate and marine environmental conditions. This study established a suitability index (SI) model and used the arithmetic average method to construct a comprehensive habitat suitability index (HSI) model based on production statistics in the northern Indian Ocean from 2017 to 2019. Variations in the suitability of habitat during different IOD events were then analyzed.

Identifying Critical Land Use Thresholds for Biodiversity Conservation in China's Lake Ecosystems.

Aquatic biodiversity loss, particularly in rapidly developing nations, continues to raise concerns, prompting urgent debates on reconciling economic growth with environmental preservation through land use planning. While spatial variations in aquatic communities along land use gradients are well-documented, precise ecological thresholds for land use impacts on freshwater lakes remain elusive, hindering sustainable development efforts. This study investigated six representative freshwater lakes in China between 2019 and 2020, all significantly impacted by anthropogenic activities.

Discovery of MK8383s with Antifungal Activity from Mangrove Endophytic Fungi sp. SCSIO 40440 Against Fusarium Wilt of Banana.

Fusarium wilt of banana (FWB), caused by f. sp. () tropical race 4 (TR4), poses a severe threat to the global banana industry.

Marine peptides in lymphoma: surgery at molecular level for therapeutic understanding.

Lymphoma, the most common form of blood cancer, affects primarily the intricate network of tissues and organs known as the lymphatic system. Globally, it ranks among the leading causes of cancer-related deaths. Although conventional therapies have led to significant advancements, they are accompanied by adverse side effects and present challenges in cases of multidrug resistance, refractory patients, and relapses.

Comprehensive analysis of microplastics at typical outlets around Hainan Island: From spatial distribution to flux estimation and correlation analysis.

Previous studies on microplastics (MPs) distribution have predominantly focused on water bodies within specific regions, with limited emphasis on the contributions of MPs directly discharged from functional zones surrounding isolated island. This study addressed this gap by investigating the occurrence and distribution of MPs in water and sediment directly discharged into the adjacent coast of Hainan Island, a geographically isolated and ecologically sensitive region in the South China Sea. Based on the sampling from 40 typical sewage outlets (affiliated to five functional zones, i.

Electrochemical biosensor based on strand displacement reaction for on-site detection of Skeletonema costatum.

An electrochemical biosensor was constructed for quantitatively detecting Skeletonema costatum (S. costatum) by combining the electrode modification material (NC-Au) with a strand displacement reaction (SDR). The SDR process addresses the issues of steric hindrance and electrostatic repulsion resulting from the large size of genomic DNA.

A Novel Strategy for the Detection of Semicarbazide in Crustaceans by Modified QuEChERS Coupled with Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry.

Semicarbazide (SEM), a metabolite of nitrofurazone (NFZ), is widely used to detect the illegal application of NFZ in crustaceans. The conventional detection method involves chemical derivatization combined with reversed-phase liquid chromatography-tandem mass spectrometry (RPLC-MS/MS), which is both complex and time-consuming. To address this limitation, a more efficient approach was developed for SEM detection.

Genetic Diversity and Environmental Adaptation Signatures of the Great Seahorse () in the Coastal Regions of the Indo-Pacific as Revealed by Whole-Genome Re-Sequencing.

The great seahorse () is one of the larger species within the seahorse group and is widely distributed in coastal areas of the Indo-Pacific. However, the natural resources of this species continue to decrease, rendering it a vulnerable species that faces a high risk of extinction. Therefore, there is an urgent need to conduct research on the genetic diversity of this species to protect its genetic resources.

Flavonoids and anthocyanins in seagrasses: implications for climate change adaptation and resilience.

Seagrasses are a paraphyletic group of marine angiosperms and retain certain adaptations from the ancestors of all embryophytes in the transition to terrestrial environments. Among these adaptations is the production of flavonoids, versatile phenylpropanoid secondary metabolites that participate in a variety of stress responses. Certain features, such as catalytic promiscuity and metabolon interactions, allow flavonoid metabolism to expand to produce novel compounds and respond to a variety of stimuli.

Identification of nutritional characteristics of Haliotis discus hannai in different China sea areas and health risks of heavy metal accumulation.

Background: The nutritional compositions of abalones (Haliotis discus hannai) vary with geographical origins and environmental factors. Traceability is an important part of food safety, and the heavy metals in the aquatic environment pose a threat to living organisms. Therefore, we present a comparative analysis of the nutrient and heavy metal content in abalones from three different sea areas in China: the Bohai Sea, East China Sea, and South China Sea.

Effect of intestinal microbiota on adaptation to overcrowding stress in grouper (Epinephelus fuscoguttatus♀×E. lanceolatus♂).

Density is an important aquaculture parameter. When the pearl gentian grouper (Epinephelus fuscoguttatus♀ × E. lanceolatus♂) is farmed intensively, it could lead to a degradation in genetic resources and an increase in disease outbreaks.