Aptasensor based on entropy-driven catalytic amplification system for the sensitive detection of acetamiprid in Chinese herbal medicine.

The traditional method of acetamiprid residue detection is difficult to operate, time-consuming, laborious and requires high professional knowledge of the detection personnel, which cannot meet the requirement of on-field rapid detection. For this reason, a colorimetric aptasensor based on an entropy-catalyzed amplification system was developed for the ultrasensitive and selective determination of acetamiprid. In the absence of acetamiprid, the aptamer and cDNA form a double-stranded structure.

Molecular mechanisms underlying floral fragrance in 'High Fragrance': a time-course assessment.

'High Fragrance' is a camellia hybrid known for its unique and intense floral scent. The current understanding of the dynamic changes in its fragrance and the underlying mechanisms are still limited. This study employed a combination of metabolomic and transcriptomic approaches to reveal the characteristics of the metabolites involved in the remarkable fragrance of this camellia and their biosynthetic mechanisms along three flower developmental stages (flower bud, initial bloom, and full bloom).

The Effect of Vegetation Ecological Restoration by Integrating Multispectral Remote Sensing and Laser Point Cloud Monitoring Technology.

This research aims to evaluate and monitor the effectiveness of vegetation ecological restoration by integrating Multispectral Remote Sensing (MRS) and laser point cloud (LPC) monitoring technologies. Traditional vegetation restoration monitoring methods often face challenges of inaccurate data and insufficient coverage, and the use of MRS or LPC techniques alone has its limitations. Therefore, to more accurately monitor the vegetation restoration status, this study proposes a new monitoring method that combines the advantages of the large-scale coverage of MRS technology and the high-precision three-dimensional structural data analysis capability of LPC technology.

Determination of the Effects of Pear- Intercropping Mode on Quality, Yield, and Soil Microbial Community.

The intercropping of in pear orchards has important production value in improving the utilization rate and economic benefits of the orchard; however, there is little research on the intercropping model of pear-. In this study, metabolomics analysis found that compared with greenhouse cultivation, there were 104 and 142 metabolites significantly increased and decreased in the intercropping mode of , respectively. Among them, there was a significant accumulation of amino acids (phenylalanine, lysine, proline, citrulline, and ornithine), sugars (arabinitol and glucosamine), and organic acids (quinic acid, fumaric acid, and malic acid) related to the unique taste of in intercropping cultivation.

Cyclic nucleotide-gated ion channel 20 regulates melatonin-induced calcium signaling and cold tolerance in watermelon.

Melatonin plays a crucial role in regulating plant cold tolerance, but the mechanisms underlying signal transduction remain elusive. In this study, we discovered that overexpression of the melatonin biosynthetic gene caffeic acid O-methyltransferase1 (COMT1) enhanced watermelon (Citrullus lanatus) cold tolerance, accompanied by the accumulation of cytosolic free calcium ([Ca2+]cyt), a stimulation of Ca2+ influx, and upregulation of four Ca2+-permeable channel genes (CNGC2/10/17/20). Conversely, knockout of COMT1 exhibited contrasting effects compared to its overexpression.

Virome of red imported fire ants by metagenomic analysis in Guangdong, southern China.

The red imported fire ant (RIFA) has made China its habitat for approximately 25 years, but few reports have described the species and amount of virus circulating in it. Researchers are currently exploring viruses associated with RIFAs as potential biological control agents against invasive ants. The present meta-transcriptome analysis revealed the virome of red imported fire ants in Guangdong, southern China, which included 17 viruses, including virus 4-GD (SINV-4) and Guangdong Polycipiviridae ant virus 1 (GPAV1) in the family; virus 1-GD (SINV-1), and Guangdong Dicistroviridae ant virus 2-3 (GDAV2-3) in the family; Guangdong Iflaviridae ant virus 4-9 (GIAV4-9) in the family; Guangdong Parvoviridae ant virus 10 (GPAV10) in the family; and Guangdong ant virus 11-15 (GAV11-15).

A newly identified photosystem II Subunit P gene in Triticeae species negatively regulates wheat powdery mildew resistance.

The photosystem II (PSII) Subunit P (PsbP) protein is a component of its oxygen-evolving complex, which can oxidize water to produce oxygen using light energy and is critical to the core components and stability of PSII. Using the whole-genome information, the genes of 10 plant species were comprehensively identified. The expression patterns of wheat s under f.

Metabolic analysis of polymeric proanthocyanidins related to red pigmentation in Camellia drupifera cv. 'Hongrou No.1' mesocarps.

Red mesocarp, characterized as a unique pigment trait of newly identified Camellia drupifera cv. 'Hongrou No.1'('HR'), is believed to act as the plant's protective shield against diverse adversities.

Acceleration of soy protein amyloid fibrils formation: Homologous seeding mechanism.

Adding prefabricated seeds to the fibril formation solution can accelerate the process of fibrillation. Various variants of soy protein amyloid fibrils (SAFs) (oligomers, short fibrils, long fibrils and mature long fibrils) were employed as seeds (OL, SF, LF and MLF). These fibrils were then introduced into a solution using a homologous seeding approach.

Pathway elucidation and heterologous reconstitution of the long-chain alkane pentadecane biosynthesis from Pogostemon cablin.

Very-long-chain (VLC) alkanes are major components of hydrophobic cuticular waxes that cover the aerial epidermis of land plants, serving as a waterproofing barrier to protect the plant against environmental stresses. The mechanism of VLC-alkane biosynthesis has been extensively elucidated in plants. However, little is known about the biosynthesis of long-chain alkanes (LC, C ~ C) such as pentadecane in plants.

The MYC Gene RrbHLH105 Contributes to Salt Stress-Induced Geraniol in Rose by Regulating Trehalose-6-Phosphate Signalling.

Rose (Rosa rugosa) is an important perfume plant, but its cultivation is significantly constrained by salt stress. Terpenes represent the most abundant volatile aromatic compounds in roses, yet little is known about how terpene metabolism responds to salt stress. In this study, salt-treated rose petals presented significant accumulation of monoterpenes, including geraniol, due to the disruption of jasmonic acid (JA) biosynthesis and signalling.

Metabolome and Transcriptome Analyses Reveal Metabolomic Variations and Key Transcription Factors Involved in Lipid Biosynthesis During Seed Development in .

Plant oils are a large group of neutral lipids that play a vital role in the food and oleochemical industries. The pecan () is a promising woody oil crop known for its high-quality sources of essential fatty acids and various bioactive compounds that may aid in preventing heart diseases. However, there is still a lack of understanding regarding the accumulation of lipids and the molecular mechanism of lipid biosynthesis during seed development.

Improving cabbage resistance to via crosses with .

Unlabelled: Cabbage is a widely cultivated leafy vegetable, but head rot disease caused by the fungus can seriously reduce its yield and quality. There are currently not any cabbage varieties that are completely immune to the disease, but its wild relative is very resistant. In this study, cabbage resistance was improved by backcrossing a highly resistant accession (C01) with a susceptible cabbage cultivar (F416).

The SBP-box transcription factor PlSPL2 negatively regulates stem development in herbaceous peony.

The SBP-box transcription factor PlSPL2 silencing in herbaceous peony enhanced stem strength by regulating xylem development, whereas its overexpression in tobacco resulted in weaker stem strength and undeveloped xylem. The strength of plant stems is a critical determinant of lodging resistance of plants, which significantly affects crop yield and cut-flower quality. Squamosa promoter binding (SBP) protein-like (SPL) transcription factors (TFs), participate in multiple regulatory processes, particularly in stem development.

The complete genome sequence of the chloroplast of .

(Asteraceae), a native of tropical America is now widespread in Asia and the Americas. We explored the chloroplast genome and conducted a phylogenetic analysis. The chloroplast genome was circular, consisting of a large single copy (LSC) of 83,909 base pairs (bp), a small single copy (SSC) of 18,407 bp, and two inverted repeat regions (IR) of 24,729 bp each.

Transcription factor PagERF110 inhibits leaf development by direct regulating PagHB16 in poplar.

Ethylene-responsive factor (ERF) family genes are crucial for plant growth and development. This study analyzed the functional role of the PagERF110 gene in leaf development of Populus alba×P. glandulosa.

Effect of protease hydrolysis on the structure of acidic heating-induced soy protein amyloid fibrils.

The effectiveness of using amyloid fibrillation to improve the functional qualities of soy protein had drawn growing attention. However, the relationship between protein subunits and the structural polymorphism of soy protein-derived amyloid fibrils (SAFs) was not yet completely understood. In this study, soy protein subunits were hydrolyzed to different degrees according to the different action sites of different proteases (Pepsin, Papain and Alcalase).

Fabrication of Novel Porous Nano-pesticides by Modifying MPN onto Cu-TCPP MOFs to Enhance Bactericidal Efficacy and Modulate Its Bioavailability.

Nano-pesticides have attracted much attention in the field of agriculture, due to existing problems such as decreased bactericidal effect and poor adhesion. An environmentally friendly metal porphyrin (Cu-TCPP)-based nanocarrier pesticide release of diniconazole (DIN) was designed to enhance bactericidal efficacy and modulate its bioavailability in a multidimensional manner by constructing a metal phenolic network (MPN) encapsulation. The introduction of the MPN prevents the DIN from prematurely escaping from the Cu-TCPP@DIN@MPN in the environment and gives it strong interfacial adhesion to resist rain washing.

The function of an apple ATP-dependent Phosphofructokinase gene MdPFK5 in regulating salt stress.

Salt stress severely affects the growth and yield of apples (Malus domestica Borkh). Although salt-tolerant genes have been extensively studied, documentation on the role of the ATP-dependent phosphofructokinase gene MdPFK5 in salt stress is limited. This study conducted an evolutionary tree and three-dimensional structure analysis of the PFK gene family in Arabidopsis thaliana and MdPFK (MD01G1037400), revealing a close phylogenetic relationship between MdPFK (MD01G1037400) and AtPFK5.

A Comparison of DNA-Methylation during Protoplast Culture of Ponkan Mandarin ( Blanco) and Tobacco ( L.).

The epigenetic variation in protoplast regeneration is a topic that has attracted interest recently. To elucidate the role of DNA methylation in the regeneration of protoplasts from the ponkan (), this study employs the methylation-sensitive amplification polymorphism (MSAP) molecular marker technique to analyze changes in DNA methylation levels and patterns during the isolation and culture of protoplasts from ponkan and tobacco. Additionally, differential DNA methylation fragments are cloned, sequenced, and subjected to bioinformatics analysis.

In Silico Discovery of a Novel PI3Kδ Inhibitor Incorporating 3,5,7-Trihydroxychroman-4-one Targeting Diffuse Large B-Cell Lymphoma.

Diffuse large B-cell lymphoma (DLBCL) is the most common lymphoma, and it is highly aggressive and heterogeneous. Targeted therapy is still the main treatment method used in clinic due to its lower risk of side effects and personalized medication. Excessive activation of PI3Kδ in DLBCL leads to abnormal activation of the PI3K/Akt pathway, promoting the occurrence and development of DLBCL.

Study on Quality and Starch Characteristics of Powdery and Crispy Lotus Roots.

Nine varieties of lotus root (, , , , , , , , and ) were selected as the research materials to compare their differences in physical, chemical, and starch characteristics before and after boiling, frying, and microwaving. The results showed that , , , , and belong to the crispy lotus root type, while , , , and belong to the powdery lotus root type. Furthermore, the nine varieties were characterized for their starch by optical micrograph (OM), polarized micrograph (PM), scanning electron micrograph (SEM), attenuated total reflection Fourier-transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), carbon-13 cross-polarization/magic angle spinning nuclear magnetic resonance (C CP/MAS NMR), and differential scanning calorimetry (DSC).