Researchers used enzymes to hydrolyze tilapia skin gelatin (TSG) to create hydrolysates with high degrees of hydrolysis and antioxidant activity, finding the best enzyme combination for optimal results.
The study measured the ability of these hydrolysates to scavenge various free radicals, determining specific IC(50) values for their effectiveness against DPPH, superoxide anion, and hydroxyl radicals.
Two notable antioxidant peptides, Glu-Gly-Leu and Tyr-Gly-Asp-Glu-Tyr, were identified with strong hydroxyl radical scavenging activities, highlighting the potential of TSG hydrolysates as sources of antioxidant peptides.