Sucrose contributed to the biofilm formation of Tetragenococcus halophilus and changed the biofilm structure.

Based on the previous research results that the addition of sucrose in the medium improved the biofilm formation of Tetragenococcus halophilus, the influence of sucrose on biofilm formation was explored. Moreover, the influence of exogenous expression of related genes sacA and galE from T. halophilus on the biofilm formation of L.

Characterizing flavor development in low-salt Chinese horse bean-chili paste through integrated metabolomics and metagenomics.

This study utilized metabolomics and metagenomics to investigate the microbial composition and functions in low- and high-salt Chinese horse bean-chili pastes (CHCPs). The results showed that 25 key metabolites were identified to distinguish the flavor attributes between the two samples. Leuconostoc was identified as the dominant microbiota in low-salt CHCP, while Pantoea prevailed in the high-salt CHCP.

Polycaprolactone/gelatin/ZIF-8 nanofiber membrane for advanced guided tissue regeneration in periodontal therapy.

The periodontal tissue regeneration strategy based on guided tissue regeneration (GTR) membranes is an effective therapy for periodontal defects. Traditional GTR membranes, however, primarily serve as physical barriers and lack antimicrobial and osteogenic functions. Herein, we developed a multifunctional nanofiber membrane with zeolitic imidazolate framework-8 nanoparticles (ZIF-8 NPs) loaded in a hydrophilic gelatin layer.

A novel polysaccharide-based bioflocculant produced by 35A and its application in the treatment of dye decolorization, heavy metal ion adsorption and meat product wastewater.

Optimizing the fermentation process of microorganisms with exceptional bioflocculant-producing capabilities is crucial for the production of bioflocculants. The application of bioflocculants to various pollutants highlights their significant advantages in water treatment. Therefore, the culture conditions of 35A with exceptional bioflocculant-producing capabilities were optimized.

Endoprotein-activating DNAzyme assay for nucleic acid extraction- and amplification-free detection of viable pathogenic bacteria.

Pathogenic bacteria in food or environment, can pose threats to public health, highlighting the requirement of tools for rapid and accurate detection of viable pathogenic bacteria. Herein, we report a sequential endoprotein RNase H2-activating DNAzyme assay (termed epDNAzyme) that enables nucleic acid extraction- and amplification-free detection of viable Salmonella enterica (S. enterica).

High-contrast imaging of cellular non-repetitive drug-resistant genes dead Cas12a-labeled PCR.

imaging of genes of pathogenic bacteria can profile cellular heterogeneity, such as the emergence of drug resistance. Fluorescence hybridization (FISH) serves as a classic approach to image mRNAs inside cells, but it remains challenging to elucidate genomic DNAs and relies on multiple fluorescently labeled probes. Herein, we present a dead Cas12a (dCas12a)-labeled polymerase chain reaction (CasPCR) assay for high-contrast imaging of cellular drug-resistant genes.

Insights into Genomic Characteristics and Biogenic Amine Degradation Potential and Mechanisms: A Strain of Sourced from .

The control of excess biogenic amines (BAs) is crucial for the sustainable development of fermented foods. This study aimed to screen endogenous functional strains in with the capacity to degrade BAs and to elucidate their application potential. L-9 (PA), which was confirmed as a safe strain by phenotypic and genotypic analyses, exhibited an efficient degradation ability on BAs, particularly regarding tyramine.

Roles of cigar microbes in flavor formation during roasted-rice leachate fermentation.

Roasted-rice leachate fermentation, a distinctive local tobacco fermentation method in Sichuan, imparts a mellow flavor and glossy texture to tobacco leaves, along with a roasted rice aroma. In order to find out the impact of roasted-rice leachate on cigar tobacco leaves, the physicochemical properties, volatile flavor profile, and microbial community were investigated. The content of protein significantly decreased after fermentation.

Modified Chitosan-Based Coating/Packaging Composites with Enhanced Antibacterial, Antioxidant, and UV-Resistant Properties for Fresh Food Preservation.

Chitosan-based biomass packaging materials are a promising material for food preservation, but their limited solubility, antioxidant capacity, UV resistance, and mechanical properties severely restrict their application. In this study, we developed a novel chitosan-based coating/packaging composite (QCTO) using quaternary ammonium salt and tannic acid (TA)-modified chitosan (QCS-TA) and oxidized chitosan (OCS). The introduction of quaternary ammonium salt and TA effectively improves the water solubility and antibacterial, antioxidant, and UV-resistant properties of chitosan.

From detection methods to risk prevention: Control of N-nitrosamines in foods and the role of natural bioactive compounds.

Food processing unavoidably introduces various risky ingredients that threaten food safety. N-Nitrosamines (NAs) constitute a class of food contaminants, which are considered carcinogenic to humans. According to the compiled information, pretreatment methods based on solid-phase extraction (SPE) were widely used before the determination of volatile NAs in foods.

Catalytic depolymerization of Camellia oleifera shell lignin to phenolic monomers: Insights into the effects of solvent, catalyst and atmosphere.

Camellia oleifera shell (COS) is a renewable biomass resource abundant in lignin with significant potential for producing phenolic monomers. However, the dearth of research has led to considerable resource wastage and environmental pollution. Herein, reductive catalytic fractionation (RCF) of COS was performed using noble metal catalysts in different solvents.

Bacterial valorization of lignin for the sustainable production of value-added bioproducts.

As the most abundant aromatic biopolymer in the biosphere, lignin represents a promising alternative feedstock for the industrial production of various value-added bioproducts with enhanced economical value. However, the large-scale implementation of lignin valorization remains challenging because of the heterogeneity and irregular structure of lignin. General fragmentation and depolymerization processes often yield various products, but these approaches necessitate tedious purification steps to isolate target products.

Novel Carboxymethyl Cellulose/Gelatin-Based Film Incorporated with Zein-Stabilized Lemon Essential Oil Pickering Emulsion for the Preservation of Cherries.

In this study, a zein-stabilized lemon essential oil Pickering emulsion (ZLPE) was incorporated into a carboxymethyl cellulose/gelatin (CMC/GL) composite film to develop a bio-based packaging material with bioactive properties. The average droplet size of the ZLPE was measured at 3.62 ± 0.

Complexation of Olive Protein with Soluble Dietary Fibers: A Way to Improve the Functional Properties of Proteins and Efficiently Utilize Olives.

High-value resources beyond oil extraction for the olive industry need to be developed due to increased olive production. Soluble dietary fibers (SDFs) and olive proteins (OPIs) are important components of olives. However, the commercial production process partially damages OPIs' emulsifying and foaming properties.

Multifunctional polymeric nanocapsules with enhanced cartilage penetration and retention for osteoarthritis treatment.

Osteoarthritis (OA) is a prevalent joint disease characterized by cartilage degeneration and subchondral bone homeostasis imbalance. Effective topical OA therapy is challenging, as therapeutic drugs often suffer from insufficient penetration and rapid clearance. We develop miniature polydopamine (PDA) nanocapsules (sub-60 nm), which are conjugated with collagen-binding polypeptide (CBP) and loaded with an anabolic drug (i.

In situ encapsulation of capsaicinoids in MIL-88A as a food-grade nanopreservative for meat safety.

The biocompatible MIL-88A metal-organic framework (MOF), synthesized from food-grade fumaric acid and ferric chloride, was introduced for the efficient one-step in situ encapsulation of capsaicinoids as a nanopreservative. The resulting MIL-88A@Caps nanoparticles can load 61.43 mg/g of capsaicinoids, surpassing conventional MOF-based encapsulation.

Ultrahigh Stretchable, Highly Transparent, Self-Adhesive, and Environment-Tolerant Chitin Nanocrystals Engineered Eutectogels toward Multisignal Sensors.

Addressing the conflict between achieving elevated mechanical stretchability and environmental adaptability is significant to a breakthrough in the practical application of flexible wearable materials. Therefore, inspired by the perceptive and protective properties of human skin, flexible wearable electronic skins (E-skins) based on deep eutectic solvent (DES) liquid and multiresponse eutectogel have been widely considered to be a promising platform for building a flexible wearable management system to achieve the purpose of "one stone, two birds". In this work, a multifunctional E-skin was designed based on an ultrastretchable, transparent, self-adhesive, and environmentally tolerant eutectogel by first incorporating cationized modified chitin nanocrystals into a covalently cross-linked polymer network comprised of the skeleton formed by a PAA polymerization network structure serving as a stretchable matrix and filled with DESs (ChCl:EG).

Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates the ZNF334 gene to inhibit the growth of colorectal cancer.

Although therapeutic targets for colorectal cancer (CRC) treatment have been developed, the treatment outcomes are not ideal and survival rates for CRC patients remain low. It is critical to identify a specific target and develop an effective CRC treatment system. The ZNF334 gene is a newly identified member of Zinc-finger proteins (ZNFs), which is essential for key biological processes associated with tumorigenesis.

N-doped lignin-based activated carbon aerogel derived from bamboo black pulp liquor for efficient removal of malachite green in wastewater.

In this study, a lignin-based aerogel (LA) was prepared through acid precipitation of BPBL, followed by sol-gel method and freeze-drying. Additionally, a one-step activation-carbonization method was used to acquire nitrogen-doped lignin-based activated carbon aerogel (NLACA). The adsorption and catalytic degradation performance for malachite green (MG) were examined.