Enhanced dark fermentative hydrogen production from waste activated sludge by combining potassium ferrate with alkaline pretreatment.

Alkaline pretreatment was demonstrated to be effective in the enhancement of hydrogen production. However, the sludge solubilization rate of alkaline pretreatment is still limited. This study reports a new strategy of KFeO + pH 9.

Screening and analysis of agouti signaling protein interaction partners in Pelodiscus sinensis suggests a role in lipid metabolism.

Agouti signaling protein (ASP) is a secreted paracrine protein that has been widely reported to function in melanogenesis and obesity and could potentially be a core protein that regulates the color and fatty phenotype of P. sinensis. In this study, we screened out interacting proteins of ASP by combined co-immunoprecipitation mass spectrometry (CoIP-MS), yeast two hybrid (Y2H) analysis, and computational predictions.

Transcriptome analysis provides insight into the role of the melanin pathway in two differently pigmented strains of the turtle Pelodiscus sinensis.

Pelodiscus sinensis is the most common turtle species raised in East and Southeast Asia. The Japanese strain and the Qingxi black strain are important aquatic breeds of P. sinensis in China with unique characteristics in terms of production performance and appearance, including skin color.

Enhanced dewaterability of anaerobically digested sludge by in-situ free nitrous acid treatment.

As the protonated form of nitrite, free nitrous acid (FNA) is a renewable chemical that can be produced on site from the anaerobic digestion liquor by nitritation, and has been widely employed to improve the fermentation of waste activated sludge (WAS). However, it is not clear whether and how FNA improves the dewaterability of anaerobically digested sludge (ADS). This work therefore aims to provide such supports through comparing the dewaterability of ADS treated by nitrite at different concentrations (0-250 mg/L) under three pH values (5.

Interaction between perfluorooctanoic acid and aerobic granular sludge.

The increasing use of perfluorooctanoic acid (PFOA) raises concerns about its potential toxicity to the environment. However, the interaction between PFOA and aerobic granular sludge has never been documented. This work therefore aims to provide such support through investigating the fate of PFOA at environmentally relevant levels in aerobic granular sludge systems and its impact on aerobic granular sludge.

Antimicrobial activity of flavonoids from L. against in culture medium and in frozen pork.

The chemical composition and antimicrobial activity of flavonoids from L. against in vitro were investigated, and the effect of flavonoid treatment on the quality of fresh pork during frozen storage for 6 months was also explored. The results showed that kaempferol, quercetin dihydrate, and catechin were the most predominant flavonoids from L.

Integration of Quantitative Trait Loci Mapping and Expression Profiling Analysis to Identify Genes Potentially Involved in Ramie Fiber Lignin Biosynthesis.

Ramie fibers, one of the most important natural fibers in China, are mainly composed of lignin, cellulose, and hemicellulose. As the high lignin content in the fibers results in a prickly texture, the lignin content is deemed to be an important trait of the fiber quality. In this study, the genetic basis of the fiber lignin content was evaluated, resulting in the identification of five quantitative trait loci (QTLs).

The Role of Anti-Müllerian Hormone in Testis Differentiation Reveals the Significance of the TGF-β Pathway in Reptilian Sex Determination.

Anti-Müllerian hormone (, or Müllerian-inhibiting substance, ), a member of TGF-β superfamily, has been well documented in some vertebrates as initiator or key regulator in sexual development, and particularly in fish. However, its functional role has not yet been identified in reptiles. Here, we characterized the gene in the Chinese soft-shelled turtle , a typical reptilian species exhibiting ZZ/ZW sex chromosomes.

sp. nov., isolated from surface seawater in the Indian Ocean.

A Gram-stain-negative bacterium, designated IO390501, was isolated from a sea water sample from the Indian Ocean and taxonomically characterized using a polyphasic approach. The strain is phylogenetically close to '' L15 and IPL18, with 16S rRNA gene sequence similarity of 97.7 and 97.

Inhibitory effect of α-ketoglutaric acid on α-glucosidase: integrating molecular dynamics simulation and inhibition kinetics.

The inhibition of α-glucosidase is used as a key clinical approach to treat type 2 diabetes mellitus and thus, we assessed the inhibitory effect of α-ketoglutaric acid (AKG) on α-glucosidase with both an enzyme kinetic assay and computational simulations. AKG bound to the active site and interacted with several key residues, including ASP68, PHE157, PHE177, PHE311, ARG312, TYR313, ASN412, ILE434 and ARG439, as detected by protein-ligand docking and molecular dynamics simulations. Subsequently, we confirmed the action of AKG on α-glucosidase as mixed-type inhibition with reversible and rapid binding.

How does zero valent iron activating peroxydisulfate improve the dewatering of anaerobically digested sludge?

Zero valent iron (ZVI) activating peroxydisulfate (PDS) was demonstrated to be effective in improving the dewaterability of anaerobically digested sludge (ADS). However, details of how ZVI/PDS enhances the dewaterability remain largely unknown. This work therefore aims to reveal the facts of what happen in ZVI/PDS involved ADS systems.

Microwave pretreatment of polyacrylamide flocculated waste activated sludge: Effect on anaerobic digestion and polyacrylamide degradation.

Deterioration of anaerobic digestion can occur with the presence of polyacrylamide (PAM) in waste activated sludge, but the information on alleviating this deterioration is still limited. In this study, the simultaneous alleviation of negative effect of PAM and improvement of methane production during anaerobic digestion was accomplished by microwave pretreatment. Experimental results showed that with the microwave pretreatment times increased from 0 to 12 min, the biochemical methane potential of PAM-flocculated sludge (12 g PAM/kg total solids) asymptotically increased from 123.

Functional study of 14-3-3 protein epsilon (YWHAE) in keratinocytes: microarray integrating bioinformatics approaches.

Previously, we detected that 14-3-3 protein epsilon (YWHAE) was involved in the pathogenesis of atopic dermatitis (AD) and tyrosinase-mediated pigmentation. In this study, we aimed to identify critical factors associated with YWHAE in human keratinocytes using high-throughput screening (HTS) approaches to reveal its functions in skin. We overexpressed YWHAE in human HaCaT keratinocytes and then conducted serial HTS studies, including RNA sequencing integrated with antibody arrays and the implementation of bioinformatics algorithms.

Found in Synanthropic Mammals from Urban Forests of Paraná, Southern Brazil.

is a parasitic protozoan that infects a diversity of hosts constituting the cycle of enzootic transmission in wild environments and causing disease in humans (Chagas disease) and domestic animals. Wild mammals constitute natural reservoirs of this parasite, which is transmitted by hematophagous kissing bugs of the family Reduviidae. is genetically subdivided into six discrete typing units (DTUs), (Tc)I to TcVI.

Prenatal gunshot wound, a rare cause of maternal and fetus trauma, a case report.

Background: Gunshot wounds in pregnant women, although rare, represent an important cause of fetal and maternal mortality. Understanding the mechanism of injury is essential to identify the possible injuries and to adequately manage the complexity of these emergency scenarios.

Case Presentation: We present a case of a 27-year-old woman and her 37 week fetus who were the victims of a gunshot wound.

Facile synthesis of 2-substituted benzo[]furans and indoles by copper-catalyzed intramolecular cyclization of 2-alkynyl phenols and tosylanilines.

A catalytic amount of CuCl and CsCO was employed to synthesize a variety of 2-substituted benzo[]furans and indoles by an intramolecular cyclization of 2-alkynyl phenols and tosylanilines. This protocol features mild conditions, high yields and broad substrate scope, which makes it a practical method for the synthesis of 2-substituted benzo[]furans and indoles.

SOHLHs Might Be Gametogenesis-Specific bHLH Transcriptional Regulation Factors in .

The self-renewal and differentiation of germ cells are essential for gametogenesis and reproduction. In mammals, the transcription factors SOHLH1 and SOHLH2, two members of the bHLH family, are specifically expressed in the gonads, and play an important role in spermatocyte and oocyte differentiation. In our previous study, we performed a phylogenetic analysis of the Lophotrochozoa bHLH genes, and two were identified in the Pacific oyster .

Devosia naphthalenivorans sp. nov., isolated from East Pacific Ocean sediment.

A Gram-stain-negative bacterium, designated CM5-1, was isolated from a sediment sample collected from the East Pacific Ocean. 16S rRNA gene sequence analysis revealed that strain CM5-1 belongs to the genus Devosia, with closely related type strains Devosia submarina KMM 9415 (98.6 %), Devosia psychrophilaCr7-05 (98.

Citrus peel flavonoids improve lipid metabolism by inhibiting miR-33 and miR-122 expression in HepG2 cells.

Citrus plants are rich in flavonoids and beneficial for lipid metabolism. However, the mechanism has not been fully elucidated. Both citrus peel flavonoid extracts (CPFE) and a mixture of their primary flavonoid compounds, namely, nobiletin, tangeretin and hesperidin, citrus flavonoid purity mixture (CFPM), were found to have lipid-lowering effects on oleic acid-induced lipid accumulation in HepG2 cells.

An OMICS-based study of the role of C3dg in keratinocytes: RNA sequencing, antibody-chip array, and bioinformatics approaches.

Previously, we have identified the C3dg protein as an important player in the pathogenesis of atopic dermatitis (AD). In this study, we aimed to identify critical factors associated with C3dg in human keratinocytes based on high-throughput screening (HTS) approaches. We overexpressed C3dg in HaCaT human keratinocytes and conducted serial HTS studies, including RNA sequencing analysis integrated with antibody-chip arrays and implementation of bioinformatics algorithms (PPI mappings).

Free nitrous acid-based nitrifying sludge treatment in a two-sludge system obtains high polyhydroxyalkanoates accumulation and satisfied biological nutrients removal.

A novel strategy to achieve substantial polyhydroxyalkanoates (PHA) accumulation in waste activated sludge (WAS) was developed, which was conducted in a two-sludge system consisted of an anaerobic/anoxic/oxic reactor (AAO-SBR) and a nitrifying reactor (N-SBR), where the nitrifying-sludge was treated by free nitrous acid (FNA). Initially, 0.98 ± 0.

Unveiling the mechanisms of how cationic polyacrylamide affects short-chain fatty acids accumulation during long-term anaerobic fermentation of waste activated sludge.

Cationic polyacrylamide, a flocculation powder widely used in wastewater pretreatment and sludge dewatering, was highly accumulated in waste activated sludge. However, its effect on short-chain fatty acids (SCFAs) accumulation from anaerobic fermentation of waste activated sludge has not been investigated. This work therefore aims to deeply unveil how cationic polyacrylamide affects SCFAs production, through both long-term and batch tests using either real waste activated sludge or synthetic wastewaters as fermentation substrates.

Gastric mixed adenoneuroendocrine carcinoma case report.

Mixed adenoneuroendocrine carcinomas are rare tumors that contain both an exocrine and an endocrine component. Since the latest classification by the World Health Organization and with the aid of immunostaining, more mixed adenoneuroendocrine carcinomas are now identified and diagnosed. Nonetheless, our knowledge of these tumors is still limited, notably concerning gastric variants, as the cases reported in the literature are very limited.

Paracoccus indicus sp. nov., isolated from surface seawater in the Indian Ocean.

Strain IO390502, isolated from surface seawater in the Indian Ocean, was characterised using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain IO390502 belongs to the genus Paracoccus and is closely related to Paracoccus seriniphilus DSM 14827 (97.6%), followed by P.