Comparative analysis of the complete chloroplast genome of seven Wikstroemia taxa (Thymelaeaceae) provides insights into the genome structure and phylogenetic relationships.

New insights into the phylogeny of species in the family Thymelaeaceae and support of the recognition of D. genkwa and D. aurantiaca as species in the genus Wikstroemia are provided.

Multiscale Mechanical Study of Proanthocyanidins for Recovering Residual Stress in Decellularized Blood Vessels.

Decellularized artificial blood vessels prepared using physical and chemical methods often exhibit limitations, including poor mechanical performance, susceptibility to inflammation and calcification, and reduced patency. Cross-linking techniques can enhance the stiffness, as well as anti-inflammatory and anti-calcification properties of decellularized vessels. However, conventional cross-linking methods fail to effectively alleviate residual stress post-decellularization, which significantly impacts the patency and vascular remodeling following the implantation of artificial vessels.

AXL: shapers of tumor progression and immunosuppressive microenvironments.

As research progresses, our understanding of the tumor microenvironment (TME) has undergone profound changes. The TME evolves with the developmental stages of cancer and the implementation of therapeutic interventions, transitioning from an immune-promoting to an immunosuppressive microenvironment. Consequently, we focus intently on the significant role of the TME in tumor proliferation, metastasis, and the development of drug resistance.

Mechanism of Action of CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in .

is an important medicinal herb; but its long-term cultivation often leads to continuous cropping problems. The underlying cause can be attributed to the accumulation of and alterations in root exudates; which interact with soil-borne pathogens; particularly ; triggering disease outbreaks that severely affect its yield and quality. It is therefore crucial to elucidate the mechanisms by which root exudates induce CCS043 outbreaks.

Intravenous administration of mitochondria improves ovarian function by anti-apoptosis in the premature ovarian insufficiency model.

Objective: For patients with contraindications to hormone therapy, the absence of effective treatments for ovarian dysfunction post chemotherapy represents a critical issue requiring resolution. Local administration of mitochondria may enhance ovarian function in premature ovarian insufficiency (POI) by ameliorating diminished mitochondrial activity. Nevertheless, there is a paucity of literature on the efficacy of mitochondrial transplantation through intravenous injection, a less invasive and more convenient method than local injection, for the improvement of ovarian function in POI following chemotherapy.

High matrix stiffness accelerates migration of hepatocellular carcinoma cells through the integrin β1-Plectin-F-actin axis.

Background: Abundant research indicates that increased extracellular matrix (ECM) stiffness significantly enhances the malignant characteristics of hepatocellular carcinoma (HCC) cells. Plectin, an essential cytoskeletal linker protein, has recently emerged as a promoter of cancer progression, particularly in the context of cancer cell invasion and metastasis. However, the responsiveness of plectin to changes in ECM stiffness and its impact on HCC progression remain unclear.

Metagenomic and metabolomic profiling analyses to unravel the formation mechanism of n-propanol during the first and second round of Jiangxiangxing Baijiu fermentation.

N-propanol is one of the higher alcohols, a moderate amount of n-propanol is beneficial for the harmony of the liquor body, whereas excessive or repeated intake will lead to discomfort and pose significant harm to human health. In actual production process of Jiangxiangxing Baijiu, the n-propanol content of the base baijiu in first round (FR) is far higher than that of second round (SR). Nevertheless, the formation mechanism and the key n-propanol producing microbials remain unclear and this limits the quality control of baijiu fermentation.

A Novel Array-Based Fluorescent Sensing Approach for the Identification and Quantification of Pesticides with High Sensitivity Based on Distinguishable Cross-response Algorithm.

The presence of excessive residues of pesticides poses a great threat to ecology and human health. Herein, a novel, low-cost, simple and precise quantification sensing platform was established for differentiating and monitoring four common pesticides in China. Particularly, the array-based ratio fluorescent sensor array detector (ARF-SAD) based on cross-reaction characteristics of porphyrins and other porphyrin derivative was successfully constructed and integrated into the platform.

Erratum: Octopod PtCu Nanoframe for Dual-Modal Imaging-Guided Synergistic Photothermal Radiotherapy: Erratum.

[This corrects the article DOI: 10.7150/thno.22557.

Photoexcited Electro-Driven Reactive Oxygen Species Channeling for Precise Extraction of Biomarker Information from Tumor Interstitial Fluid.

Direct electrochemical detection of miRNA biomarkers in tumor tissue interstitial fluid (TIF) holds great promise for adjuvant therapy for tumors in the perioperative period, yet is limited by background interference and weak signal. Herein, a wash-free and separation-free miRNA biosensor based on photoexcited electro-driven reactive oxygen channeling analysis (LEOCA) is developed to solve the high-fidelity detection in physiological samples. In the presence of miRNA, nanoacceptors (ultrasmall-size polydopamine, uPDA) are responsively assembled on the surface of nanodonors (zirconium metal-organic framework, ZrMOF) to form core-satellite aggregates.

YY2-CYP51A1 signaling suppresses hepatocellular carcinoma progression by restraining de novo cholesterol biosynthesis.

Lipid accumulation is a frequently observed characteristic of cancer. Lipid accumulation is closely related to tumor progression, metastasis, and drug resistance; however, the mechanism underlying lipid metabolic reprogramming in tumor cells is not fully understood. Yin yang 2 (YY2) is a C2H2‑zinc finger transcription factor that exerts tumor-suppressive effects.

Antibacterial mechanism of SHY96 cell-free supernatant against revealed by metabolomics and potential application on chicken breast meat preservation.

The cell-free supernatant of (LCFS) is considered a potential natural antimicrobial agent due to its outstanding antimicrobial activity. This study demonstrated that the cell-free supernatant of SHY96 (LCFS96) effectively inhibits the growth and biofilm formation of CMCC(B)54002 (_02) by reducing cell metabolic activity and damaging cell structure. Metabolomic analysis revealed that LCFS96 significantly altered 450 intracellular metabolites, affecting key metabolic pathways including linoleic acid metabolism, pyrimidine metabolism, purine metabolism, pantothenic acid and CoA biosynthesis, and the TCA cycle.

Solvent induced method for preparation of multi-advantage carrageenan films.

Carrageenan has good film-forming characteristics, but it is difficult to simultaneously improve its multiple performances, such as water-resistance, light transmittance and thermal stability. In this study, multi-advantage composite films were prepared by iota-carrageenan and quaternary ammonium surfactants according to solvent induced method. The weight change, FTIR and thermogravimetric analyses of the films before and after solvent inducement indicated that the inorganic counterions of iota-carrageenan were replaced by quaternary ammonium ions.

Nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction.

Here, we developed nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction (NanaPPI). When an interaction occurs, primary antibodies for two proteins bring the proximity of secondary nanobody-fused small/large fragment to reassemble into an intact NanoLuc variant, thus transforming interaction events to luminescent signals in situ with high sensitivity. Compared to proximity ligation assay, NanaPPI has a similar signal-to-background ratio, but it is more convenient with faster procedures, easier readout and lower cost.

Optimizing electrode configurations for EEG mild cognitive impairment detection.

The Optimal electrode configuration of Electroencephalograms (EEG) systems for mild cognitive impairment (MCI) detection and monitoring in non-clinical settings, i.e. number of electrodes and the positions of the electrodes, remains to be explored.

An antibacterial and antioxidant rosmarinic acid hydrogel normalizes macrophage polarization to expedite diabetic wound healing.

The management of diabetic wounds (DW) is a significant challenge within the medical field. Effectively regulating the levels of reactive oxygen species (ROS) at the wound site and orchestrating the inflammatory response are effective strategies for DW treatment. In this study, a novel hydrogel was developed by cross-linking polyboronic acid-modified carboxymethyl chitosan with herbal active ingredient rosmarinic acid (RA), an active herbal ingredient, through dynamic boronic esters formation.

Dual DNAzyme amplification-based colorimetric sensing assay for the identification and quantification of tumor-associated miRNAs.

Herein, we present a colorimetric sensing strategy for the identification and quantification of tumor-associated miRNAs based on dual DNAzyme amplification. In this sensing ensemble, the substrate portion of the Pb-dependent 8-17 DNAzyme combines with the G-quadruplex portion to form a hairpin substrate strand. The two split 8-17 DNAzyme strands are partially complementary to the substrate strand and serve as a recognition unit for binding the target miRNA.

2D Flower-like CdS@Co/Mo-MOF as Co-Reaction Accelerator of g-CN-Based Electrochemiluminescence Sensor for Chlorpromazine Hydrochloride.

In this study, we have proposed an electrochemiluminescence (ECL) signal amplification system which is based on two-dimensional (2D) flower-like CdS@Co/Mo-MOF composites as a co-reaction accelerator of the g-CN/SO system for ultrasensitive detection of chlorpromazine hydrochloride (CPH). Specifically, the 2D flower-like Co/Mo-MOF with mesoporous alleviated the aggregation of CdS NPs while simultaneously fostering reactant-active site contact and improving the reactant-product transport rate. This allowed the material to act as a novel co-reaction accelerator, speeding up the transformation of the SO into SO and enhancing the cathodic ECL emission of g-CN.

Restoration of vascular dysfunction resulting from maternal high-fat diet via modulation of the NLRP3/IL-1β axis.

This study investigated the impact of maternal high-fat diet on vascular function and endothelial homeostasis in offspring. We found that offspring exposed to maternal high-fat diet exhibited elevated blood pressure, impaired abdominal aortic vascular function, and endothelial homeostasis imbalance. These changes were accompanied by increased levels of reactive oxygen species (ROS) and upregulation of pro-inflammatory cytokines (including IL-1β, TNF-α, IL-6, and IL-10).

Cordycepin ameliorates spaceflight-induced osteoporosis by preventing BMSCs oxidative stress and senescence via interacting with PI3K p110α and regulating PI3K/Akt/FOXO3 signalling.

Spaceflight-induced osteoporosis (SFOP) is a detrimental healthcare consequence during spaceflight. Weightlessness and ionizing radiation were main environmental factors that contribute to SFOP, especially in the manned deep space voyages. However, currently there is scarce effective method to treat SFOP.

A One-Stone-Two-Birds Strategy for Intervertebral Disc Repair: Constructing a Reductive Chelation Hydrogel to Mitigate Oxidative Stress and Promote Disc Matrix Reconstruction.

Intervertebral disc degeneration (IVDD) is characterized by fibrosis of nucleus pulposus (NP) cells and accelerated surrounding extracellular matrix catabolism. Bioactive hydrogels have shown significant potential in regulating cellular functions and tissue homeostasis. In this work, a dynamic hydrogel (HA-NCSN/Cu) is designed via the reductive chelation of hyaluronic acid grafted with thiourea (HA-NCSN) and Cu.

An integrated wearable microfluidic biosensor for simultaneous detection of multiple biomarkers in sweat.

Simultaneous detection of biomarkers in sweat is crucial for comprehensive health assessment and personalized monitoring. However, the low sweat secretion rate and low metabolite concentrations present challenges for developing non-invasive wearable sensors. This study aims to develop a flexible wearable biosensor for simultaneous detection of low-concentration biomarkers in sweat, enabling comprehensive health assessment.

Extracellular Vesicle-Like Nanovesicle for Skin Photoaging Therapy.

Ultraviolet (UV) irradiation leads to the degradation of the extracellular matrix and collagen, thereby accelerating skin aging and imposing substantial psychological burden on patients. Current anti-aging strategies are limited and often associated with high costs or strong side effects. Plant-derived extracellular vesicle-like nanovesicles, with advantages such as natural availability and cost-effectiveness, show potential in anti-aging interventions.

Involvement of copper in cell death and cancer.

Copper (cu) is an essential micronutrient required for numerous metabolic processes. It plays a crucial role in cellular respiration by participating in the electron transport chain and facilitating numerous biological reactions. Various diseases, including cancer, demonstrate localized elevation of copper levels and/or alterations in the overall distribution of copper.