Cyanidin-3-O-glucoside ameliorates hydrogen peroxide-induced oxidative stress by regulating HMGCR-mediated cholesterol anabolism in HEK-293T cells.

Cyanidin-3-O-glucoside (C3G), as a typical anthocyanin, exhibits excellent antioxidant effects. This study aimed to demonstrate the role and mechanism of C3G in regulating 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR)-mediated cholesterol anabolism on HO-induced oxidative stress in HEK-293T cells. Firstly, the inhibitory effect of C3G on oxidative stress was confirmed by CCK-8, ROS, and mitochondrial membrane potential (MMP) experiments.