Effects of rocky desertification on soil bacterial community in alpine grasslands of the Qinghai-Tibet Plateau.

The makeup of soil microbial communities may serve as a crucial predictor of the alpine grassland ecosystem. Climate change and human disturbance have resulted in intensified ecosystem degradation, such as grassland rocky desertification, which may modify the structures and composition of the microorganisms. However, little is known about the effects of rocky desertification on soil microbial communities of soil.

The characteristics of intestinal flora of Tibetan sheep in different regions at high altitude were revealed based on metagenomic technique.

Tibetan sheep play a vital role in the livelihoods of herders and are an important part of the ecosystem of the Tibetan Plateau. In order to study the characteristics of the gut microorganisms of Tibetan sheep at high altitude, this study employed macrogenomic techniques to analyse the diversity and differences in the gut flora of Tibetan sheep in different regions of high altitude and high cold. The results demonstrated that at the phylum level, the dominant phylum in the ileo-cecum segment of Tibetan sheep in Qilian, Henan and Gonghe counties was identical, namely , and .

A novel pigeon paramyxovirus type 1 isolated from a sick racing pigeon in the Qinghai-Tibet Plateau of China shows high virulence in chickens.

Pigeon paramyxovirus type-1 (PPMV-1) is the causative agent of pigeon Newcastle disease (ND), which has caused huge losses to the pigeon industry. In this study, a PPMV-1 strain, PPMV-1/QH-01/CH/23, was isolated from a sick racing pigeon in the Qinghai-Tibet Plateau, China in 2023. The mean death time of chicken embryos and the intracerebral pathogenicity index (ICPI) were 76.

Characteristics of the Bacterial Community in Alpine Meadows in Response to Altitude and Aspect in the Qilian Mountains, Northwest China.

As one of the most sensitive and fragile alpine ecosystems in the Qilian Mountains, the alpine meadow holds significant scientific importance in understanding the changes in the characteristics of soil bacterial community in response to altitude and aspect variations. In our study, we analyzed the composition, diversity, and function of soil bacterial communities in alpine meadows at different altitudes and aspects and their relationship with environmental factors. Our results indicate that altitude and aspect orientation significantly influences the diversity index and composition of soil bacterial communities.

Investigating the Rhizosphere Fungal Communities of Healthy and Root-Rot-Infected in the Tsaidam Basin, China.

is a plant of considerable economic importance in China. However, root rot poses a significant threat to its yield and quality, leading to substantial economic losses. The disparities in rhizosphere soil fungal communities between healthy and root-rot-affected have not been thoroughly explored.

Pharmacological Mechanisms of Bile Acids Targeting the Farnesoid X Receptor.

Bile acids (BAs), a category of amphiphilic metabolites synthesized by liver cells and released into the intestine via the bile duct, serve a vital role in the emulsification of ingested fats during the digestive process. Beyond their conventional emulsifying function, BAs, with their diverse structures, also act as significant hormones within the body. They are pivotal in facilitating nutrient absorption by interacting with the farnesoid X receptor (FXR), and they serve as key regulators of lipid and glucose metabolism, as well as immune system balance.

Resveratrol and β-hydroxy-β-methylbutyric acid supplementation promotes ileal development and digestive function by altering microbial community abundance and metabolites in Tibetan sheep.

Introduction: The effects of resveratrol (RES) and β-hydroxy-β-methylbutyric acid (HMB) on phenotype, immunity, digestive enzyme activity and short-chain fatty acids (SCFAs) contents in ileum of Tibetan sheep were investigated.

Methods: A total of 120 two-month-old Tibetan sheep (15.5 ± 0.

Effect of miR-10a on the proliferation and differentiation of yak adipocyte precursors.

The fat content of yak meat is significantly correlated with the meat quality, and an appropriate fat content helps to improve the texture of the meat. The involvement of miR-10a in regulating the differentiation and proliferation of various cell types has been reported. Therefore, in this study, the effects of miR-10a on lipid droplet accumulation were investigated by transfection of yak adipocyte precursors with an miR-10a inhibitor, followed by Oil Red O, BODIPY, EdU staining, and cell cycle analysis of the transfected and control cells.

Impact of Lysine to Methionine Ratios on Antioxidant Capacity and Immune Function in the Rumen of Tibetan Sheep: An RNA-Seq Analysis.

With global protein prices on the rise, lowering protein levels in animal feed, together with balancing diet composition and reducing nitrogen emissions, can both reduce the environmental impact of agriculture and save on feed costs. However, the formulation of an ideal amino acid (AA) composition is crucial for better protein utilization by livestock. This study aimed to investigate the effects of different lysine to methionine ratios on the antioxidant capacity and immune function of the rumen in Tibetan sheep.

Comparative RNA sequencing analysis of three Capripoxvirus infections in an immortalized hTERT-bOEC cell model.

Capripoxviruses (CaPVs), such as lumpy skin disease, sheep pox, and goat pox, cause significant production and economic losses and are major constraints to the growth of livestock production in endemic areas. Understanding the pathogenic mechanism of CaPVs and their translation into clinical applications depends on the availability of a suitable cell line. In this study, we used a lentiviral packaging system to establish an immortalized hTERT-bOEC cell line by ectopic introduction of human telomerase reverse transcriptase (hTERT).

Genome-Wide Identification of the GPAT Family in L. and Expression Profiling Under Abiotic Stress.

Glycerol-3-phosphate acyltransferase (GPAT), as a rate-limiting enzyme engaged in lipid synthesis pathways, exerts an important role in plant growth and development as well as environmental adaptation throughout diverse growth stages. Alfalfa ( L.) is one of the most significant leguminous forages globally; however, its growth process is frequently exposed to environmental stress, giving rise to issues such as impeded growth and decreased yield.

Metabolomics Characterization of Chemical Composition and Bioactivity of Highland Barley Monascus Tea Decoction Before and After Simulated Digestion In Vitro.

A broadly targeted metabolomics approach based on UPLC-MS/MS was employed to investigate the changes in chemical composition and in vitro activity of highland barley Monascus tea decoction before and after simulated digestion. The characteristic metabolites of the tea decoction before and after in vitro-simulated digestion were identified, and the in vitro antioxidant and enzyme inhibitory activities of the tea decoction were further analyzed. The study detected 1431 metabolites, including amino acids and their derivatives, alkaloids, organic acids, nucleotides and their derivatives, lipids, terpenoids, and phenolic acids.

Co-application of sheep manure and commercial organic fertilizer enhances plant productivity and soil quality in alpine mining areas.

Background And Aims: The addition of organic fertilizers and sheep slat manure have important effects on soil quality in alpine mining areas, but how they affect soil physicochemical properties and microorganisms is not yet known.

Methods: The current study employed field-controlled experiments and high-throughput sequencing technology to investigate differences in soil physicochemical properties, microbial community structures, and diversity under four treatments: no fertilization (CK), 100% sheep manure (SM), a combination of 50% sheep manure and 50% commercial organic fertilizer (MF), and 100% commercial organic fertilizer (OF).

Results: Aboveground biomass increased by 191.

Ingesting Significantly Impacts the Gastrointestinal Tract Bacterial Community and Diversity in Plateau Zokors ().

Intestinal bacteria are considered the "second genome" of the host, playing a crucial physiological role in assisting the host in degrading plant secondary compounds, nutrient absorption, immune regulation, and other aspects. To explore the effects of on the bacterial community of the gastrointestinal tract of plateau zokor, this study uses the 16S rRNA gene high-throughput sequencing technology, and the biodiversity and the community structure of gut bacteria in different gastrointestinal tract segments (the stomach and cecum) of plateau zokors. The results showed that at the phylum level, the dominant flora in the stomach and cecum of plateau zokors before and after ingesting were and Bacteroidetes.

Multitrophic Diversity of the Biotic Community Drives Ecosystem Multifunctionality in Alpine Grasslands.

Biodiversity and ecosystem multifunctionality are currently hot topics in ecological research. However, little is known about the role of multitrophic diversity in regulating various ecosystem functions, which limits our ability to predict the impact of biodiversity loss on human well-being and ecosystem multifunctionality. In this study, multitrophic diversity was divided into three categories: plant, animal, and microbial communities (i.

Large-scale metagenomic assembly provide new insights into the genetic evolution of gut microbiomes in plateau ungulates.

Trillions of microbes colonize the ungulate gastrointestinal tract, playing a pivotal role in enhancing host nutrient utilization by breaking down cellulose and hemicellulose present in plants. Here, through large-scale metagenomic assembly, we established a catalog of 131,416 metagenome-assembled genomes (MAGs) and 11,175 high-quality species-level genome bins (SGBs) from 17 species of ungulates in China. Our study revealed the convergent evolution of high relative abundances of carbohydrate-active enzymes (CAZymes) in the gut microbiomes of plateau-dwelling ungulates.

Environmental Factors Drive the Biogeographic Pattern of Root Endophytic Fungal Diversity in the Arid Regions of Northwest China.

subsp. Rousi (Abbrev. ) stands as a vital botanical asset in ameliorating the ecological landscape of the arid regions in Northwest China, where its rhizospheric microorganisms serve as linchpins in its growth and developmental dynamics.

The development of hooded awns in barley: From ectopic Kap1 expression to yield potential.

Awns in barley have different shapes including awnless, straight, hooded, crooked, and leafy awns. The hooded awns are characterized by an appendage of the lemma, which forms a trigonal or cap-shaped structure, and even blossoms and yields fruits on barley awn. In the lemma primordia of wild-type (straight awn), cells divide and elongate to form the straight awn.

UV-B irradiation promotes anthocyanin biosynthesis in the leaves of .

Anthocyanins are the most valuable pigments in (). Although ultraviolet-B (UV-B) irradiation is a key environmental factor influencing anthocyanin biosynthesis in , the deep molecular mechanism remains unclear. Herein, we examined the changes in the total anthocyanin content and transcriptomic characteristics of leaves following UV-B irradiation treatment.

Role of molybdenum in ameliorating busulfan-induced infertility in female mice.

Background: Molybdenum (Mo) plays a crucial role in regulating normal physiological function. However, its potential effect on female infertility has received little attention.

Methods: In this study, we explored the potential molecular mechanisms of Mo's action on mouse ovaries and oocytes by establishing a busulfan-induced infertility model.

Immune, Oxidative, and Morphological Changes in the Livers of Tibetan Sheep after Feeding Resveratrol and β-Hydroxy-β-methyl Butyric Acid: A Transcriptome-Metabolome Integrative Analysis.

This study investigated the effects of dietary resveratrol (RES) and β-Hydroxy-β-methyl butyric acid (HMB) on immune, oxidative, and morphological changes in the livers of Tibetan sheep using transcriptomics and metabolomics. One hundred and twenty male Tibetan lambs of a similar initial weight (15.5 ± 0.