Identification of Two New HLA-DQA1 Alleles: HLA-DQA1*01:173 and -DQA1*03:82.

HLA-DQA1*01:173 and -DQA1*03:82, two novel HLA-DQA1 alleles detected by next generation sequencing.

Identification of three new HLA-A intronic variants by next-generation sequencing.

Three novel HLA-A intronic variants, HLA-A*03:01:01:121, HLA-A*29:02:01:41 and HLA-A*30:02:01:24, detected by next-generation sequencing.

Characterisation of two new HLA-DPA1 alleles: HLA-DPA1*02:139 and -DPA1*02:140N.

HLA-DPA1*02:139 and -DPA1*02:140N, two novel HLA-DPA1 alleles detected by next-generation sequencing.

Identification of six new HLA-C variants with nucleotide changes in non-coding regions.

Six novel HLA-C variants with nucleotide changes in non-coding regions: HLA-C*04:32:01:02, -C*07:01:01:142, -C*08:04:01:05, -C*12:03:01:68, -C*12:03:01:69 and -C*16:01:01:41.

Identification of the novel HLA-C*08:291 allele by next-generation sequencing.

HLA-C*08:291, a novel HLA class I allele detected by next-generation sequencing.

Identification of the novel HLA-DQA1*05:03:03 allele by next-generation sequencing.

HLA-DQA1*05:03:03, a novel HLA class II allele detected by next-generation sequencing.

A novel HLA-DPA1 allele, HLA-DPA1*02:134, identified by next-generation sequencing.

HLA-DPA1*02:134, a novel HLA class II allele detected by next-generation sequencing.

Risk factors for posttraumatic stress disorder in trauma patients from bullfighting-related events in Spain.

Background: Unintentional Trauma injuries are one of the leading causes of posttraumatic stress disorder development. However, screening for this condition is not typically included in routine medical care. Research on posttraumatic stress disorder after unintentional injuries sustained during entertainment activities involving risk, has been scarce.

Recurrent genetic variants and prioritization of variants of uncertain clinical significance associated with hereditary breast and ovarian cancer in families from the Region of Murcia.

Objectives: Hereditary breast and ovarian cancer (HBOC) follows an autosomal dominant inheritance pattern of cancer susceptibility genes. The risk of developing this disease is primarily associated with germline mutations in the and genes. The advent of massive genetic sequencing technologies has expanded the mutational spectrum of this hereditary syndrome, thereby increasing the number of variants of uncertain clinical significance (VUS) detected by genetic testing.

The prognostic impact of SIGLEC5-induced impairment of CD8 T cell activation in sepsis.

Background: Sepsis is associated with T-cell exhaustion, which significantly reduces patient outcomes. Therefore, targeting of immune checkpoints (ICs) is deemed necessary for effective sepsis management. Here, we evaluated the role of SIGLEC5 as an IC ligand and explored its potential as a biomarker for sepsis.

Measuring NLR Oligomerization III: Detection of NLRP3 and NLRC4 Complex by Bioluminescence Resonance Energy Transfer.

Bioluminescent resonance energy transfer (BRET) is a natural phenomenon resulting from a non-radiative energy transfer between a bioluminescent donor (Renilla luciferase) and a fluorescent protein acceptor. BRET signal is dependent on the distance and the orientation between the donor and the acceptor and could be used to study protein-protein interactions and conformational changes within proteins at real-time in living cells. This protocol describes the use of BRET technique to study NLRP3 oligomerization in living cells before and during NLRP3 inflammasome activation.

Identification of a novel HLA-DPA1 allele, HLA-DPA1*02:102Q.

HLA-DPA1*02:102Q, a novel HLA class II allele detected by next-generation sequencing.

Exploring Urban Green Spaces' Effect against Traffic Exposure on Childhood Leukaemia Incidence.

Background: Several environmental factors seem to be involved in childhood leukaemia incidence. Traffic exposure could increase the risk while urban green spaces (UGS) exposure could reduce it. However, there is no evidence how these two factors interact on this infant pathology.

Identification of five new HLA-DQB1 intronic variants by next-generation sequencing.

Five novel HLA-DQB1 intronic variants detected by next-generation sequencing: HLA-DQB1* 03:01:01:53, -DQB1*03:01:01:55, -DQB1*03:02:01:17, -DQB1*03:02:01:18 and - DQB1*06:03:01:20.

Characterization of two new HLA class I alleles: HLA-B*35:572 and HLA-C*04:491.

HLA-B*35:572 and HLA-C*04:491, two novel HLA class I alleles detected by next-generation sequencing.

Characterization of two new HLA-DPB1 alleles: HLA-DPB1*1443:01 and -DPB1*11:01:07.

HLA-DPB1*1443:01 and -DPB1*11:01:07, two novel HLA-DPB1 alleles detected by next generation sequencing.

Hepatitis B viral infection and role of alcohol.

End-stage liver disease is frequently caused by hepatitis B virus (HBV) and alcohol consumption. Notably, the mechanism by which alcohol affects the course of HBV-associated liver disease is unknown, and additional research is needed in this area. A reduced immunological response, oxidative stress, endoplasmic reticulum stress, Golgi apparatus stress, and enhanced HBV replication are a few potential causes.

Characterization of P2X7 Receptors in Human Blood Cells.

P2X7 receptors are expressed in different circulating immune cells. Depending on the cell in which they are expressed, their function could vary from activation of the leucine-rich repeat receptor pyrin domain containing 3 (NLRP3) inflammasome or the shedding of different surface receptors. The P2X7 receptor has been involved in the evolution of several inflammatory diseases, and thus its characterization in blood samples is important to investigate its role in human diseases.

Exploring Blue Spaces' Effects on Childhood Leukaemia Incidence: A Population-Based Case-Control Study in Spain.

Background: Blue spaces have been a key part of human evolution, providing resources and helping economies develop. To date, no studies have been carried out to explore how they may be linked to paediatric oncological diseases.

Objectives: To explore the possible relationship of residential proximity to natural and urban blue spaces on childhood leukaemia.

CA 15-3 prognostic biomarker in SARS-CoV-2 pneumonia.

The severity of lung involvement is the main prognostic factor in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Carbohydrate antigen 15-3 (CA 15-3), a marker of lung damage and fibrosis, could help predict the prognosis of SARS-CoV-2 pneumonia. This was a retrospective and observational study.

Assessment of ASC Oligomerization by Flow Cytometry.

Inflammasomes are multiprotein complexes that critically control different aspects of innate and adaptive immunity. Upon activation, inflammasome proteins oligomerize forming scaffolds to nucleate the apoptosis-associated speck-like protein containing a CARD (ASC) in filaments that will finally result in large ASC oligomers that are commonly named as ASC specks. In this chapter, we present a method to monitor NLRP3 or pyrin inflammasome activation in human monocytes upon extracellular ATP or Clostridium difficile toxin B treatment, respectively, by detecting intracellular oligomers of ASC by flow cytometry.

Extracellular NLRP3 inflammasome particles are internalized by human coronary artery smooth muscle cells and induce pro-atherogenic effects.

Inflammation driven by intracellular activation of the NLRP3 inflammasome is involved in the pathogenesis of a variety of diseases including vascular pathologies. Inflammasome specks are released into the extracellular compartment from disrupting pyroptotic cells. The potential uptake and function of extracellular NLRP3 inflammasomes in human coronary artery smooth muscle cells (HCASMC) are unknown.

Urban green spaces and childhood leukemia incidence: A population-based case-control study in Madrid.

Background: Childhood leukemia is the most common childhood cancer. To date, few risk factors related to predisposition have been identified; therefore, new hypotheses should be considered.

Objective: To explore the possible relationship of residential proximity to urban green spaces on childhood leukemia.