4 results match your criteria: "Clinical Medical School of Hangzhou Normal University[Affiliation]"

Objective: To investigate the effects of polysaccharide fraction of Cordyceps sinensis (PSCS) on triptolide (TPL)-induced apoptosis in the HL-60 cells and the involved molecular mechanism.

Methods: The cultured leukemia HL-60 cells were divided into three groups: control group, TPL group (cells were treated with 5 ng/ml TPL only), and PSCS+TPL cells group (cells treated with 5 ng/ml TPL and 100 microg/ml or 200 microg/ml PSCS for 18 h). Cell viability was tested by MTT assay and apoptotic cells were quantitatively measured by flow cytometry with Annexin V/PI double stain.

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Article Synopsis
  • The study aimed to analyze the distribution of Haemophilus influenzae serotypes and their resistance to ampicillin among children in Hangzhou with respiratory infections.
  • A total of 152 isolates were collected, with 97.4% untypable and a small percentage being typeable; none were types b or c.
  • Most isolates remained susceptible to ampicillin, though 22.4% were resistant and some were found to produce beta-lactamase.
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Objective: To investigate the mechanism underlying the anticancer activity of cucurbitacin B on human laryngeal cancer.

Method: Hep-2 cells were treated with different concentrations of cucurbitacin B for different time. MTT assay was used to evaluate cell proliferation.

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Inhibitory effects of cucurbitacin B on laryngeal squamous cell carcinoma.

Eur Arch Otorhinolaryngol

October 2008

Department of Otolaryngology, Clinical Medical School of Hangzhou Normal University, Hangzhou 310015, Zhejiang, People's Republic of China.

Cucurbitacins are compounds isolated from various plant families, which have been used as folk medicines for centuries in countries such as India and China because of their wide spectrum of pharmacological activities such as cytotoxic, anti-inflammatory, and anticancer effects. Accumulated evidences have shown that cucurbitacin B inhibits the growth of numerous human cancer cell lines and tumor xenografts. To determine whether cucurbitacin B can inhibit the growth of laryngeal squamous cell carcinoma, in the present study we investigated the antitumor effect of cucurbitacin B on Hep-2 cells.

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