Chitosan-PLGA polymer blends as coatings for hydroxyapatite nanoparticles and their effect on antimicrobial properties, osteoconductivity and regeneration of osseous tissues.

Composite biomaterials comprising nanostructured hydroxyapatite (HAp) have an enormous potential for natural bone tissue reparation, filling and augmentation. Chitosan (Ch) as a naturally derived polymer has many physicochemical and biological properties that make it an attractive material for use in bone tissue engineering. On the other hand, poly-D,L-lactide-co-glycolide (PLGA) is a synthetic polymer with a long history of use in sustained drug delivery and tissue engineering.

GLUT2 and TAS1R2 Polymorphisms and Susceptibility to Dental Caries.

Objective: Dental caries is one of the most frequent multifactorial diseases. Among the numerous factors influencing the risk of caries, genetics plays a substantial role, with heritability ranging from 40 to 60%. Gene variants affecting taste preference and glucose transport were recently associated with caries risk.

Apolipoprotein E gene polymorphisms in relation to chronic periodontitis, periodontopathic bacteria, and lipid levels.

Objective: Inflammatory periodontal diseases may be associated with common systemic conditions and, as recently described, alterations in lipid levels in the blood. The aim of this study was to determine the possible effects of apolipoprotein E (ApoE) genotypes on the lipid levels in healthy people and patients with chronic periodontitis (CP) in relation to periodontopathic bacteria.

Design: This case-control study comprised 469 unrelated subjects.

The effect of IL-4 gene polymorphisms on cytokine production in patients with chronic periodontitis and in healthy controls.

Chronic periodontitis (CP) is an inflammatory disease of the teeth-supporting tissues in which genetic predisposition, dental plaque bacteria, and immune mechanisms all play important roles. The aim of this study was to evaluate the occurrence of IL-4 gene polymorphisms in chronic periodontitis and to investigate the association between polymorphisms and cytokines production after bacterial stimulation. Sixty-two subjects (47 CP patients and 15 healthy controls) with detected two polymorphisms in the IL-4 gene (-590C/T and intron 3 VNTR) were examined.

The titanium PV I endosteal implant from beta-titanium alloy Ti 38Nb 6Ta.

Aim: The aim of this study was to use the beta-titanium alloy Ti38Nb6Ta for production of a new construction line of implants, perform testing on animals and preclinical tests.

Materials And Methods: Within this study, a new PV I implant with five construction variants was developed. The implant includes three types of threads - microthreads and flat threads of two types with a different depth.

Stromal reaction and prognosis in acinic cell carcinoma of the salivary gland.

Introduction: Primary acinic cell carcinoma (ACC) is an uncommon malignant neoplasm of the salivary gland (SG), which usually presents as slow growing tumor.

Case Report: We reported a 69-year-old woman with tumor in the right parotid gland with a 5-year progress. Biopsy sections revealed a hybrid form of ACC with a low- and high-grade component and prominent lymphoid tissue in tumor stroma.

Haplotype analysis of interleukin-8 gene polymorphisms in chronic and aggressive periodontitis.

Objectives: Periodontitis is an inflammatory disease characterized by connective tissue loss and alveolar bone destruction. Interleukin-8 (IL8) is important in the regulation of the immune response. The aim of this study was to analyze four polymorphisms in the IL8 gene in relation to chronic (CP) and aggressive (AgP) periodontitis.

Lack of association between lactotransferrin polymorphism and dental caries.

Objective: Dental caries is a complex, multifactorial disease and one of the most common illnesses worldwide. Its etiology is related to microbial, dietary and host factors. Recent evidence suggests a role of lactotransferrin (LTF) in caries.

Aspects of orthodontic-prosthetic rehabilitation of dentofacial anomalies.

Skeletal class III malocclusion is one of the most difficult dentofacial anomalies, characterized by deviation in the development of the mandible and maxilla in the sagittal plane, where the mandible is dominant in relation to the maxilla. In patients with class III malocclusion, anomalies in the dentoalveolar level and esthetic discrepancies are also frequent. The etiology of class III malocclusion is multifactorial due to the interaction of hereditary and environmental factors.

Actinic cheilitis and squamous cell carcinoma of the lip: clinical, histopathological and immunogenetic aspects.

Actinic cheilitis is the main precancerous lesion of the lip. Squamous cell carcinoma of the lip is reported together with oral carcinomas in the Brazilian official statistics. Overall, they account for 40% of the head and neck carcinomas.

Matrix metalloproteinase 8 (MMP8) gene polymorphisms in chronic periodontitis.

Objective: Previous studies have suggested that some functional polymorphisms in the matrix metalloproteinase (MMPs) genes are associated with the risk of periodontal disease. However, to date no study has investigated MMP8 gene variants in relation to chronic periodontitis (CP). The aim of this study was to analyse polymorphisms in the MMP8 gene and their associations with microbial composition and clinical manifestation of CP.

Dentoskeletal characteristics in patients with palatally and buccally displaced maxillary permanent canines.

The aim of this retrospective study was to analyse dentoskeletal characteristics in patients with eruption disturbances of the maxillary permanent canines. Pre-treatment panoramic radiographs and lateral cephalograms of 636 consecutive orthodontic patients were assessed. The control group included 456 patients with physiologically erupted maxillary permanent canines (261 females and 195 males, mean age 12.

The use of absorbable polyglactin/polydioxanon implant (Ethisorb(®)) in non-surgical closure of oro-antral communication.

Oro-antral communications (OAC) greater than 4-5 mm in diameter can seldom be counted on to heal spontaneously without the necessity for surgical closure. The initial experience in applying an absorbable polyglactin/polydioxanon implant (Ethisorb(®)), in non-surgical closure of OAC ranging from 5 to 7 mm in diameter, is presented. Twelve patients of varying ages with OAC up to 72 h in duration, have been treated with Ethisorb(®).

Root resorption associated with ectopically erupting maxillary permanent canines: a computed tomography study.

The aims of this retrospective computed tomography (CT) study were to determine the occurrence of severe root resorption involving the pulpal canal of adjacent permanent teeth associated with ectopically erupting canines, and to verify the existence of related factors. The sample consisted of 255 consecutive patients (159 females and 96 males, mean age 18.4 and 16.

Interferon-γ +874A/T polymorphism in relation to generalized chronic periodontitis and the presence of periodontopathic bacteria.

Background: Interferon gamma (IFN-γ) is one of the key regulatory cytokines that has a significant effect on immune responses. It may be important in the chronic inflammatory diseases such as periodontitis in which increased IFN-γ levels were found. The aim of this study was to analyze +874A/T polymorphism in the IFN-γ gene and its associations with the presence of periodontopathic bacteria and susceptibility to generalized chronic periodontitis (CP).

Association of Toll-like receptor 9 haplotypes with chronic periodontitis in Czech population.

Aim: Toll-like receptors (TLRs) belong to the pattern recognition receptors family of signal molecules that recognize conserved microbial structures. The aim of this study was to analyse polymorphisms in the TLR genes and their association with chronic periodontitis (CP).

Material And Methods: Two polymorphisms (2408G/A, i.

Effects of fixed orthodontic appliances on subgingival microflora.

Objectives: Fixed orthodontic appliances cause plaque accumulation around bands and brackets. Since the microbiological composition of dental plaque is closely connected to periodontal tissue health, the aim of this study was to determine the effects of fixed orthodontic appliances on subgingival microflora and periodontal status.

Methods: This prospective study was carried out on 32 adolescents scheduled for fixed orthodontic treatment.

Clinical and microbiological effects of fixed orthodontic appliances on periodontal tissues in adolescents.

Objective: To determine the effects of fixed orthodontic appliances on periodontal health and microbiological composition of subgingival dental plaque.

Material And Methods: This prospective longitudinal self-controlled study was conducted on 32 adolescents (13 males, 19 females), who were scheduled for fixed orthodontic treatment between 2002 and 2005. Dental plaque accumulation, gingival inflammation and pocket probing depth were measured at the mesio-vestibular angle of the examined group of teeth followed by collection of subgingival dental plaque samples in the same points.

Lack of association between chronic periodontitis and the Toll-like receptor 4 gene polymorphisms in a Czech population.

Background And Objective: Periodontitis is a bacterially induced chronic inflammatory disease and a major cause of tooth loss among adults. Toll-like receptors are signal molecules essential for the cellular response to bacterial cell wall components. The aim of this study was to investigate relationships between chronic periodontitis and variations in the TLR4 gene.

Substitution of osteoporotic alveolar bone by biphasic calcium phosphate/poly-DL-lactide-co-glycolide biomaterials.

Lost bone tissue due to osteoporosis makes dentistry very difficult. The aim of thisstudy is to reconstruct the bone tissue with composite biomaterials and to estimate the optical density and alveolar ridge height of the mandible. Research is conducted on 30 postmenopausal women aged from 46 to 62 years, with diagnosed osteoporosis and defects in alveolar bones caused by extraction of paradontopathic teeth, enucleation of cysts and periapical changes, extraction of impacted teeth,or by trauma.

The assessment of anesthetic efficacy of ropivacaine in oral surgery.

This paper describes the application of ropivacaine, local, amid, long-acting anesthetic in oral surgery. For infiltrative anesthesia, 0.75% of Naropin (ropivacaine) was used in eight patients undergoing various operations (maxillary sinus with oro-antral communication, extraction of upper and lower impacted wisdom teeth, cystectomy, apicoectomy and tooth extraction).

Repair of bone tissue affected by osteoporosis with hydroxyapatite-poly-L-lactide (HAp-PLLA) with and without blood plasma.

The aim of this study is to examine the reparatory ability of the synthetic biomaterial hydroxyapatite-poly-L-lactide (HAp-PLLA), the replacement of alveolar ridge, and rehabilitation of bone defects caused by osteoporosis, in an experimental group of animals. The experiments are performed on syngeneic Sprague Dawley rats. Osteoporosis is induced by glucocorticoids in rats during a 12-week period.

Investigation of the bone tissue response to glass-ionomer microimplants in the canine maxillary alveolar ridge.

This paper reports the results of experimental use of glass-ionomer microimplants in the augmentation of the maxillary alveolar ridge in dogs. The study included ten adult mongrel dogs 5 years of age, weighing between 50 and 70 pounds (25-30 kg), divided into 2 groups of 5 dogs each. In both groups, the maxillary 4th premolar and 1st molar were removed after the elevation of a buccal mucoperiosteal flap.

Polymorphisms in the +252(A/G) lymphotoxin-alpha and the -308(A/G) tumor necrosis factor-alpha genes and susceptibility to chronic periodontitis in a Czech population.

Background: Periodontitis is an inflammatory disease that leads to irreversible attachment loss, bone destruction and eventually tooth loss. Tumor necrosis factor (TNF), a pluripotent proinflammatory cytokine that is able to induce tissue destruction and bone resorption, has been implicated in the pathogenesis of periodontal disease.

Methods: In this study, we investigated an association between chronic periodontitis and two previously described bi-allelic polymorphisms in the TNF locus: a G to A transition at position -308 in the 5'promoter region of the TNF-alpha gene and an NcoI restriction fragment length polymorphism (RFLP) in the first intron (position +252A/G) of the lymphotoxin alpha (LT-alpha) gene.