Human reproductive failure is not a clinical feature associated with beta(2) glycoprotein-I antibodies in anticardiolipin and lupus anticoagulant seronegative patients (the antiphospholipid/cofactor syndrome).

It has been suggested that patients with clinical features suggestive of antiphospholipid syndrome but being lupus anticoagulant (LA) and anticardiolipin (aCL) negative, should be tested for antibodies to beta(2) glycoprotein-I (abeta(2)GP-I), a protein involved in the binding of antiphospholipid antibodies (aPL) to phospholipid surfaces. This was investigated in the present study where a total of 385 women aged View Article and Find Full Text PDF

Recurrent empty follicle syndrome successfully treated with recombinant human chorionic gonadotrophin.

We report a case of a patient with polycystic ovary syndrome and primary infertility who was admitted to our in-vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) programme because of her partner's severe oligozoospermia and asthenozoospermia. Ovarian stimulation was accomplished in the three treatment cycles using gonadotrophin therapy after a dual approach with ovarian suppression using oral contraceptive pills followed by gonadotrophin-releasing hormone agonist therapy. Oocyte retrieval was unsuccessful in the first two treated cycles despite the fact that human chorionic gonadotrophin (HCG) from three different batches was used.

Amplification and expression of the c-erbB-2 oncogene in Müllerian-derived genital-tract tumors.

This study focused on 18 uterine corpus tumors and 7 ovarian endometrioid tumors, all of them malignant and Müllerian derived. Differential polymerase chain reaction (DPCR), dot blot hybridization, and immunohistochemical technique were employed to determine c-erbB-2 amplification and expression. Of 25 Müllerian-derived tumors, 17 (68.

Neoadjuvant chemotherapy followed by radical hysterectomy and postoperative adjuvant chemotherapy in the treatment of carcinoma of the cervix uteri: long-term follow-up of a pilot study.

Objective: The aim of the study was to determine if ACH given after NCH followed by RH could decrease the incidence of distant metastases in patients with locally advanced carcinoma of cervix uteri.

Material: 56 pts (34 Ib, 18 IIb, 4 IIIb) with confirmed diagnosis of squamous cervical cancer were enrolled in this phase II trial. The methodology used was: 1) Figo clinical staging; 2) Ultrasonographic determination of tumor volume in < or > 4 cms; 3) V.

p53 is a persistent and predictive marker in advanced ovarian carcinomas: multivariate analysis including comparison with Ki67 immunoreactivity.

p53 mutation and p53 protein overexpression are common findings in ovarian carcinomas. In order to evaluate the prognostic significance of the p53 status and its role in metastasis, we examined 104 ovarian carcinomas, among them 83 cases with follow-up data, and 40 pairs of primary tumors and metastases, by p53 immunohistochemistry and temperature-gradient gel electrophoresis. Comparison of primary tumors and their metastases revealed identical results in 88%-90% of the cases, indicating that, in most cases, mutant p53 occurs prior to metastatic spread and remains clonally conserved.

Accumulation of 5-aminolevulinic acid-induced protoporphyrin IX in normal and neoplastic human endometrial epithelial cells.

The aim of this study was to evaluate 5-Aminolevulinic acid (ALA)-induced fluorescence of normal and neoplastic endometrial epithelial cells for diagnosis and photodynamic treatment. Fluorescence of ALA-induced PpIX in vitro was measured by flow cytometry in two different human endometrial adenocarcinoma cell lines and in normal cells cultivated from fresh endometrial tissue of three premenopausal patients. The cells were analysed after incubation with different concentrations of ALA during 3, 6, or 24 hours.

The estimation of fructosamine and HbAlc in pregnant women with diabetes mellitus.

Fructosamine, HbAlc, glucose, albumins and total proteins were estimated in 40 healthy pregnant women and 80 pregnant women with insulin dependent diabetes mellitus. Fructosamine was estimated by the NBT method with "Fructosamine test" commercially available kit on Technicom automatic analyser RA-1000. Glucose was determined on Beckman glucose analyser.

Surgical treatment of vulvar cancer.

Two hundred and seventy two women with vulvar cancer were submitted to standard surgical treatment. In 127 women (46.7%) regional lymph nodes were involved.

Doppler sonographic examinations of uteroplacental, fetoplacental, and fetal hemodynamics and their prognostic value in preterm labor.

Doppler examinations of different uteroplacental vessels (uterine arteries, arcuate arteries), umbilical artery, fetal thoracic aorta, and median cerebral artery were performed on 55 patients with idiopathic preterm labor (24.5 to 32.5 weeks).

Alpha 2 macroglobulin in healthy pregnant women and those with EPH gestosis.

The Authors determined alpha 2 macroglobulin in 50 healthy pregnant women and in 50 pregnant women with EPH gestosis, using the technique of nephelometry on the Immunochemistry Analyzer. The assays were done in blood serum of the mother and umbilical cord, urine and in the amniotic fluid of the affected patients. Serum values were expressed in gr/L, and amniotic fluid and urine values in mg/L.

Asymptomatic Herpesvirus hominis (HVH) cervicovaginal infection in normal and high risk pregnancy.

Asymptomatic genital Herpesvirus hominis (HVH) infection in a group of 100 pregnant women with normal and high risk pregnancy was examined. Vaginal and cervical HVH shedding was diagnosed by the method of immunofluorescence. The results of cervical and vaginal swabs taken from the same patients, showed that in both groups of pregnant women examined, the presence of HVH infection in the vagina and cervix was not always simultaneous.

Histochemical demonstration of a delta 5,3 beta-hydroxysteroid dehydrogenase activity of cumulus cells related to the maturity and developmental potential of recovered oocytes.

A simple and rapid histochemical technique is described for demonstration of delta 5,3 beta-hydroxysteroid dehydrogenase activity in cumulus cells from preovulatory follicles aspirated for in vitro fertilization (IVF) of corresponding oocytes. Histochemical activity of delta 5,3 beta-hydroxysteroid dehydrogenase was demonstrated in samples of cumulus obtained from 62 oocytes recovered from 24 women. Patients were treated with clomiphene citrate in combination with human menopausal gonadotropins and human chorionic gonadotropin injections.

Zinc status in women with premature rupture of membranes at term.

Zinc concentrations were measured by atomic absorption spectrometry in whole blood, scalp hair, pubic hair, and colostrum from patients at term with and without premature rupture of membranes (PROM). A maternal zinc index was established for each patient, expressed as an average ranking of the four determinations. The mean +/- SD value of the maternal zinc index in patients with PROM was significantly lower than in patients without this complication (4.

Protein concentration in pre-ovulatory follicular fluid related to ovarian stimulation.

Sixty follicular fluids obtained from 26 women with either clomiphene citrate and human menopausal gonadotropins (hMG) or hMG-induced ovulation were analyzed for the contents of total proteins, fibrinogen, plasminogen, antithrombin III, ceruloplasmin, alpha-2 macroglobulin, alpha-1 antitrypsin and immunoglobulins (IgG, IgA, IgM). Concentrations of these proteins was correlated to the type of ovarian follicle growth induction. Follicular fluids from patients stimulated with clomiphene citrate-hMG contained significantly higher concentrations of ceruloplasmin than those treated with hMG alone.

The complements and immunoglobulins in different media of healthy pregnant women and in pregnant women with increased blood pressure.

We have determined the levels of complement C3, C4 and immunoglobulin G, M, A in mothers' and cord blood serum. Parallelly properdin factor B and immunoglobulin G tests were done in urine samples. All estimations were performed on Immunochemistry Analyzer "Beckman".

Diabetes mellitus/male infertility.

Diabetes mellitus (DM), associated with very subtle disorders, affects, either directly or indirectly, various functions of the reproductive system. Adequate, regular, and timely therapy may prevent or delay these disorders. The T synthesis disorder is caused by molecular changes at the level of Leydig cells and may lead to other disorders in all target organs and tissues.

Human colostrum as a source of organohalogen xenobiotics for a breast-fed neonate.

The concentrations of p,p'-isomers of DDT, DDE, and DDD, and alpha, beta, and gamma isomers of hexachlorohexane (HCH), hexachlorobenzene (HCB), polychlorinated biphenyls (PCBs) were determined by means of gas-liquid chromatography in 3/4 postpartum day colostrum of 54 normal women. The milk levels of t-DDT, t-HCH, HCB, and PCBs correlated significantly with one another. The contents in milk of all the studied organohalides significantly increased with maternal age.

A new case of deletion 1q42 syndrome.

We report a 1 8/12-year-old male with a de novo deletion of 1q42. The case is compared with 23 others from the literature. The clinical manifestations of our patient correspond with the phenotype of previous reports.

Diagnostic and therapeutic procedures in the vulvar intraepithelial neoplasia (VIN) and early invasive cancer of the vulva.

The study comprised 71 women from 1st Clinic of Gynecology Medical Academy of Cracow from 30 to 76 years (means = 52.5) with VIN and early invasive cancer of the vulva. The clinical examination, colposcopy, cytology, histology of target biopsy and culture of microorganisms taken from the vulva were performed in all cases.

Lipid metabolism during treatment of endometriosis with the progestin dienogest.

The effects of a new progestational compound, dienogest (17 alpha-cyanomethyl-17 beta-hydroxy-estra-4,9-dien-3-one) on lipid metabolism have been studied in 84 otherwise healthy women with laparoscopically proven endometriosis. The women, aged 17 to 45, years were treated with 2 mg dienogest in tablet form daily for 24 weeks. The progestin was highly effective on endometriotic lesions and symptoms, showing an objective endoscopic and a subjective symptomatic improvement in 80% and 83% respectively.

The significant of complement and immunoglobulin determination in healthy pregnant women and patients with EPH gestoses.

Complements C3 and C4, IgG, IgM, IgA and properdin factor B (PFB) were determined in maternal blood sera, sera from the umbilical cord and amniotic fluid in the group consisting of 30 healthy pregnant women and 30 patients with EPH gestoses. PFB and IgG were measured in urine, as well. Significant decrease of C3 complement in maternal sera and PFB in urine was found in the group with EPH gestoses, while a slight C4 complement enhancement was recorded in both maternal sera and the blood sera from the umbilical cord.

Cord blood serum creatine kinase isoenzymes with placental dysfunction.

It has been suggested that perinatal asphyxia is not generally followed by neurological impairment unless there is preexisting chronic fetal distress. In cases of brain damage one can observe elevated levels of CK-BB. The purpose of our study was to evaluate CK isoenzymes in umbilical cord blood sera of newborns affected by chronic fetal distress.