A diazirine's central carbon is sp-hybridized, facilitating conjugation to dye molecules.

Diazirines are versatile carbene precursors that are extensively used in biological target identification experiments. However, their photo-activation wavelength ( 365 nm) precludes their use in living organisms. Here we show that a reconceptualization of the diazirine hybridization state leads to conjugation of the diazirine motif to longer-wavelength chromophores.

Diameter distribution by deconvolution (DdD): absorption spectra as a practical tool for semiconductor nanoparticle PSD determination.

Semiconductor nanoparticles (SNPs) are excellent candidates for various applications in fields like solar cells, light emitting diodes or sensors. Their size strongly determines their properties, thus characterizing their size is crucial for applications. In most cases, they are included in complex matrices which make it difficult to determine their average diameter and statistical distribution.

Studies on uroporphyrinogen decarboxylase from Chlorella kessleri (Trebouxiophyceae, Chlorophyta).

Uroporphyrinogen decarboxylase (UroD) (EC 4.1.1.

Possible role of carbonic anhydrase, V-H(+)-ATPase, and Cl(-)/HCO3- exchanger in electrogenic ion transport across the gills of the euryhaline crab Chasmagnathus granulatus.

We studied the participation of carbonic anhydrase (CA), V-H(+)-ATPase, and Cl(-)/HCO3- exchanger in electrogenic ion absorption through the gills of Chasmagnathus granulatus. CA activity was measured in anterior gills and posterior gills after acclimation to 2 per thousand, 10 per thousand, 30 per thousand (about seawater), and 45 per thousand salinity. The highest CA specific activity was detected in the microsomal fraction in anterior gills, and in the cytosolic fraction, in posterior ones.

Modulation of ion uptake across posterior gills of the crab Chasmagnathus granulatus by dopamine and cAMP.

Cyclic AMP (cAMP) and dopamine modulate ion uptake across isolated and perfused posterior gills of Chasmagnathus granulatus acclimated to 10 per thousand salinity. Addition of cAMP agonists, such as cp-cAMP, forskolin, and IBMX, produced a significant increase in the transepithelial potential difference (Vte), which reflects ion transport activity. Dopamine (DA) also had a stimulatory effect on ion uptake, increasing Vte and Na(+) influx, although this effect was transient, since both variables remained elevated for less than 30 min.

Light emission diode water thermometer: a low-cost and noninvasive strategy for monitoring temperature in aqueous solutions.

A spectroscopic device for monitoring the temperature of aqueous solutions is presented. It uses a 950 nm light emission diode as light source and two photodiodes as detectors. Temperature is monitored following the thermally induced absorbance changes of the water-OH second overtone (approximately 960 nm).