1 results match your criteria: "Chulalongkorn University Pathumwan Bangkok 10330 Thailand manchumas.h@chula.ac.th prath@chula.ac.th thanapon.charoenwongpaiboon@gmail.com.[Affiliation]"
Rational re-design of 121 inulosucrase for product chain length control.
RSC Adv
May 2019
Department of Biochemistry, Faculty of Science, Chulalongkorn University Pathumwan Bangkok 10330 Thailand
Article Synopsis
- Fructooligosaccharides (FOSs) are prebiotics useful in various industries, and inulosucrase can be engineered to synthesize different FOS chain lengths from sucrose.
- Three engineered variants of inulosucrase (R483F, R483Y, R483W) showed improved binding to a specific substrate, leading to higher production of shorter chain FOSs (GF5) compared to the wild type.
- Even though the engineered variants displayed reduced overall catalytic activity, they could still achieve FOS synthesis yields of up to 73% from the substrate, confirming the effectiveness of the modifications.