Proteomic characterization and comparison of the infective and adult life stage secretomes from Necator americanus and Ancylostoma ceylanicum.

More than 470 million people globally are infected with the hookworms Ancylostoma ceylanicum and Necator americanus, resulting in an annual loss of 2.1 to 4 million disability-adjusted-life-years. Current infection management approaches are limited by modest drug efficacy, the costs associated with frequent mass drug administration campaigns, and the risk of reinfection and burgeoning drug resistance.

Botulinum Toxin-loaded Detachable Dissolvable Microneedles for Primary Axillary Hyperhidrosis: A Pilot Study on Efficacy and Safety.

Objective: Botulinum toxin injection is a well-established treatment for primary hyperhidrosis. The botulinum toxin-loaded detachable dissolvable microneedles (BoNT-MNs) were developed in a result of disadvantages of the intradermal procedure. This pilot study aims to evaluate the efficacy and safety of BoNT-MNs.

Metabolomic profiling and antibacterial efficacy of probiotic-derived cell-free supernatant encapsulated in nanostructured lipid carriers against canine multidrug-resistant bacteria.

Aim: This study aimed to investigate the antibacterial efficacy of probiotic-derived cell-free supernatants (CFS) encapsulated within nanostructured lipid carriers (NLCs) against multidrug-resistant and . Additionally, it aimed to identify specific bioactive compounds that contribute to the reported antibacterial properties by characterizing the metabolite substances present in the CFS using a metabolomic analysis technique.

Methods: Eight strains of lactic acid bacteria including (L22F and L25F), (P72N, BF9, BF 14, BYF 20 and BYF 26) and (BF 12) were selected as probiotic candidates.

Coinfection of and porcine circovirus type 3 is linked to reproductive failure in pig farms.

Background And Aim: Reproductive disorders in swine herds pose significant challenges to pig breeding due to both infectious and non-infectious factors. In large-scale pig farming, coinfections are increasingly common, affecting sow health and herd productivity. This study aimed to determine occurrence and coinfection patterns of s and porcine circovirus type 3 in Vietnamese pig farms and to evaluate their association with reproductive disorders and clinical signs in affected herds.

Identification of veterinary and medically important blood parasites using contrastive loss-based self-supervised learning.

Background And Aim: Zoonotic diseases caused by various blood parasites are important public health concerns that impact animals and humans worldwide. The traditional method of microscopic examination for parasite diagnosis is labor-intensive, time-consuming, and prone to variability among observers, necessitating highly skilled and experienced personnel. Therefore, an innovative approach is required to enhance the conventional method.

Effects of dog cardiac orientation on vertebral heart score measurements in different thoracic types.

Background And Aim: Computed tomographic (CT) images can elucidate the variations of cardiac orientation that this information among dog breeds has never been reported. This study aimed to explore the heart orientations of dogs with different thoracic types and study their effects on vertebral heart score (VHS) measurements using CT images.

Materials And Methods: Thoracic CT images of 115 mature dogs without thoracic abnormalities were retrospectively examined.

Highly Porous 3D Ni-MOFs as an Efficient and Enzyme-Mimic Electrochemical Sensing Platform for Glucose in Real Samples of Sweat and Saliva in Biomedical Applications.

Nickel-based metal-organic frameworks, denoted as three-dimensional nickel trimesic acid frameworks (3D Ni-TMAF), are gaining significant attention for their application in nonenzymatic glucose sensing due to their unique properties. Ni-MOFs possess a high surface area, tunable pore structures, and excellent electrochemical activity, which makes them ideal for facilitating electron transfer and enhancing the catalytic oxidation of glucose. This research describes a new electrochemical enzyme-mimic glucose biosensor in biological solutions that utilizes 3D nanospheres Ni-TMAF created layer-by-layer on a highly porous nickel substrate.

The ability of different forms of autogenous tooth graft to promote bone regeneration: a network meta-analysis.

Purpose: Several forms of autogenous tooth graft have been presented. However, it is still unclear which form provides better bone formation and is the best to use clinically. This network meta-analysis aimed to thoroughly evaluate the available literature on the ability of different forms of the autogenous tooth graft to promote bone regeneration, in order to explore if any specific type or method of processing would result in better overall outcomes.

Laparoscopic repair of Bochdalek hernia with intrathoracic kidney and concomitant redo-redo incisional hernia.

Bochdalek hernias (BHs), though rare, are the most common congenital diaphragmatic hernias. Their coexistence with an ectopic intrathoracic kidney (IK), found in 0.25% of cases, is even rarer.

Outdoor air pollution exposure and the risk of type 2 diabetes mellitus: A systematic umbrella review and meta-analysis.

The association between different air pollutants and Type 2 Diabetes Mellitus (T2DM) is a growing topic of interest in public health research. This umbrella review and meta-analysis aimed to consolidate current literature on the association between various outdoor air pollutants and T2DM. Subgroups and dose-response relationships were also analyzed to further quantify the association, especially by the factors such as the type of pollutants, duration of exposure, and geographical variation, etc.

Sustained Improvement in Medical Students' Academic Performance in Renal Physiology Through the Application of Flipped Classroom.

Background: Teaching renal physiology is problematic in many medical schools since conventional passive learning might not be effective. Active learnings including flipped classroom (FC) have been introduced to medical education including renal physiology topic recently, but no study regarding long-term outcomes has been reported.

Approach: Two classes of second-year medical students were compared.

Spoilage investigation of pasteurized apple juice with visual defects identifies a potentially novel Acetobacter species as the primary spoilage agent.

Jellified materials were observed in spoiled pasteurized apple juice that contained dimethyl dicarbonate (DMDC). Microbiological analysis showed a high microbial load (4-5 log CFU/mL) in the sample. Acetobacter spp.

Acute kidney injury.

Acute kidney injury (AKI) is a common, heterogeneous, multifactorial condition, which is part of the overarching syndrome of acute kidney diseases and disorders. This condition's incidence highest in low-income and middle-income countries. In the short term, AKI is associated with increased mortality, an increased risk of complications, extended stays in hospital, and high health-care costs.

Unveiling the Impact of Shrimp piRNAs on WSSV Infection and Immune Modulation.

Piwi-interacting RNAs (piRNAs) are small non-coding RNAs that play a crucial role in gene regulation and immune defense. This study investigates their function in Penaeus vannamei shrimp during White Spot Syndrome Virus (WSSV) infection. Analysis of small RNA libraries from WSSV-infected shrimp hemocytes identified 82,788 piRNA homologs, with 138 showing altered expression during infection.

Multiscale Engineered Bionic Solid-State Electrolytes Breaking the Stiffness-Damping Trade-Off.

All-solid-state lithium metal batteries are regarded as next-generation devices for energy storage due to their safety and high energy density. The issues of lithium dendrites and poor mechanical compatibility with electrodes present the need for developing solid-state electrolytes with high stiffness and damping, but it is a contradictory relationship. Here, inspired by the superstructure of tooth enamel, we develop a composite solid-state electrolyte composed of amorphous ceramic nanotube arrays intertwined with solid polymer electrolytes.

Medication underuse in real-life practice: the impact of galcanezumab towards achieving very low frequency episodic migraine in a southeast Asian middle-income nation.

Background: Migraine progression, particularly from episodic to chronic migraine (CM), increases disease burden and healthcare costs. Understanding the new concept of "Medication Underuse Headache" should encourage the health care provider to consider early intervention with calcitonin gene-related peptide (CGRP) monoclonal antibodies. Galcanezumab given early in the course of the disease, may prevent migraine chronification and have a robust response, moreso than when initiated in later stages of migraine.

Structure and catalytic activity of a dihydrofolate reductase-like enzyme from Leptospira interrogans.

A dihydrofolate reductase (DHFR)-like enzyme from Leptospira interrogans (LiDHFRL) was cloned and the recombinant protein was characterized. Sequence alignment suggested that the enzyme lacked the conserved catalytic residues found in DHFR. Indeed, LiDHFRL did not catalyze the reduction of dihydrofolate by either NADH or NADPH.

Quercetin-functionalized nanomaterials: Innovative therapeutic avenues for Alzheimer's disease management.

Alzheimer's Disease (AD) is a major global health challenge, largely due to its complex pathology and the limited effectiveness of existing treatments. Quercetin, a bioactive compound belonging to the flavonoid class, its promising antioxidant, anti-inflammatory, and neuroprotective effects in addressing AD. However, its therapeutic potential is hindered by challenges such as low bioavailability, instability, and restricted permeability across the blood-brain barrier (BBB).

Exploring mimosamycin as a Janus kinase 2 inhibitor: A combined computational and experimental investigation.

Janus kinases (JAKs) are a family of intracellular tyrosine kinases that play a crucial role in signal transduction pathways. JAK2 has been implicated in the pathogenesis of leukemia, making it a promising target for research aimed at reducing the risk of this disease. This study examined the potential of mimosamycin as a JAK2 inhibitor using both in vitro and in silico approaches.

Detecting freezing of gait: A comprehensive toolkit for enhanced Parkinson's assessment.

Introduction: Detecting Freezing of Gait (FOG) poses challenges, with the subjective 6-item FOG Questionnaire relying solely on patient perception. We aim to create a holistic FOG Detection Toolkit combining subjective and objective elements (descriptions, images, and videos) to improve FOG detection precision.

Methods: Development of the FOG Detection Toolkit involved a detailed cover sheet on FOG and its triggers, along with video exemplars and a 4-item FOG-specific self-assessment questionnaire, all rigorously validated.

Exploring the anti-aging potential of natural products and plant extracts in budding yeast : A review.

Historically, plant derived natural products and their crude extracts have been used to treat a wide range of ailments across the world. Biogerontology research aims to explore the molecular basis of aging and discover new anti-aging therapeutic compounds or formulations to combat the detrimental effects of aging and promote a healthy life span. The budding yeast has been, and continues to be, an indispensable model organism in the field of biomedical research for discovering the molecular basis of aging has preserved nutritional signaling pathways (such as the target of rapamycin (TOR)-Sch9 and the Ras-AC-PKA (cAMP-dependent protein kinase) pathways, and shows two distinct aging paradigms chronological life span (CLS) and replicative life span (RLS).

Prediction of axillary nodal burden using preoperative magnetic resonance imaging scoring in patients with clinically node-negative breast cancer: a retrospective cohort study.

Background: Axillary lymph node metastasis (ALNM) is a significant predictor of overall patient survival; thus, precise evaluation of ALNM is essential for staging breast cancer, informing multimodal treatment strategies, and ensuring optimal patient care. This study aimed to establish a magnetic resonance imaging (MRI) scoring system for predicting extensive axillary nodal metastasis in patients with clinically node-negative breast cancer derived from preoperative breast and axillary MRI.

Methods: This study included 226 patients with clinically node-negative breast cancer who underwent preoperative breast and axillary MRI between January 1, 2010 and December 31, 2020 at King Chulalongkorn Memorial Hospital.

Eligibility Criteria of Randomized Clinical Trials in Critical Care Medicine.

Importance: Eligibility criteria for randomized clinical trials (RCTs) are designed to select clinically relevant patient populations. However, not all eligibility criteria are strongly justified, potentially excluding marginalized groups, and limiting the generalizability of trial findings.

Objective: To summarize and evaluate the justification of exclusion criteria in published RCTs in critical care medicine.

Long-term excessive salt consumption alters villous and crypt morphology and the protein expression of uroguanylin, TRPV6 and PMCA1b in the rat small intestine.

Although long-term high dietary sodium consumption often aggravates hypertension and bone loss, sodium in the intestinal lumen has been known to promote absorption of nutrients and other ions, e.g., glucose and calcium.