[Characterization of microstructure of ibuprofen-hydroxypropyl-beta-cyclodextrin and ibuprofen-beta-cyclodextrin by atomic force microscope].

The microstructures of ibuprofen-hydroxypropyl-bets-cyclodextrin (IBU-HP-beta-CyD) and ibuprofen-beta-cyclodextrin (IBU-beta-CyD) were observed by atomic force microscope (AFM). The high resolving capability of AFM has the tungsten filament probe with the spring constant of 0.06 N x m(-1).

[Pilot study of PTEN deletion affecting the expression of Cu/Zn SOD].

Objective: To investigate whether the deletion of PTEN affects the expression of Cu/Zn SOD and the related biology.

Methods: Protein and mRNA expression levels of PTEN, P-Akt, Cu/Zn SOD in the control immortalized wild type mouse embryonic fibroblast cells (PTEN+/+) and PTEN-null cells (PTEN-/-) were evaluated by Western blot and Northern blot respectively. The level of superoxide anions were detected using fluorescent probes.

Effects of electroacupuncture on expressions of angiogenesis factors and anti-angiogenesis factors in brain of experimental cerebral ischemic rats after reperfusion.

Objective: To explore the mechanism of electroacupuncture (EA) in improving ischemic stroke.

Methods: The Wistar rat model of focal cerebral ischemia-reperfusion was prepared by the thread embolism method. The rats were randomly divided into a normal group, a model group, and an EA group.

Characteristics of circulating T cell receptor gamma-delta T cells from individuals chronically infected with hepatitis B virus (HBV): an association between V(delta)2 subtype and chronic HBV infection.

Background: To date, few studies have been conducted to determine whether T cell receptor (TCR) gammadelta T cells are involved in hepatitis B virus (HBV) infection. This study was performed to assess the quantity and immune function of TCRgammadelta T cells in the blood of patients with chronic HBV infection and to analyze the relationship between proportions of TCRgammadelta T cells and both proportions of other immune cells and clinical parameters.

Methods: Flow cytometry was used to detect the proportions of TCRgammadelta T cells and other immune cells in the peripheral blood of 46 asymptomatic carriers (AsCs) of HBV, 95 patients with chronic hepatitis B (CHB), and 29 healthy donors (HDs).

[Expression of the hepatic insulin signal transduction proteins in the rats with intrauterine growth retardation during the perinatal period].

Objective: To investigate the effects of maternal malnutrition on the expression of the insulin signal transduction proteins in the liver of perinatal rats.

Methods: (1) The dams were semi-starvation from the first day during pregnancy to build the animal model of intrauterine growth retardation (IUGR). On days 15, 17, 19, 21(E15, E17, E19, E21) of gestation, the fetuses were delivered by cesarean section.

Relationship between TH1/TH2 cytokines and immune tolerance in liver transplantation in rats.

Objective: To explore the relationship between Th1/Th2 cytokine expressions and immune tolerance in rat liver transplantation.

Methods: Recipients were divided into three groups (each group = 12 rats). The allograft group underwent orthotopic liver transplantation from male Wistar-to-Sprague-Dawley (SD) rats.

[Effects and the mechanism of HGF on the apoptosis of rat primary cultured hepatic stellate cells treated with platelet-derived growth factor].

Objective: To investigate the inhibitory effect of hepatocyte growth factor (HGF) on the apoptosis of rat primary cultured hepatic stellate cells treated with platelet-derived growth factor BB (PDGF-BB).

Methods: Hepatic stellate cells were cultured and treated with PDGF-BB+HGF, HGF or nothing (controls). Apoptosis of these hepatic stellate cells was evaluated by AO/EB staining, TUNEL and flow cytometry.

The effect of different treatment strategies on left atrial size in patients with lone paroxysmal atrial fibrillation-a prospective cohort study.

Introduction: The effect of different treatment strategies of atrial fibrillation on left atrial (LA) size has not been compared in lone paroxysmal atrial fibrillation. The objective of the present study was to evaluate the evolution of LA size over time in patients who underwent different treatment interventions.

Methods And Results: Two hundred forty patients with lone paroxysmal atrial fibrillation were assigned to four groups.

High-intensity focused ultrasound (HIFU): effective and safe therapy for hepatocellular carcinoma adjacent to major hepatic veins.

Hepatocellular carcinoma (HCC) is an especially frequent malignancy in China. Radiofrequency ablation, percutaneous ethanol injection, transarterial chemoembolization, cryoablation, microwave coagulation, and laser-induced interstitial thermotherapy all offer potential local tumor control and occasionally achieve long-term disease-free survival. High-intensity focused ultrasound (HIFU), as a noninvasive therapy, can be applied to treat tumors that are difficult to treat with other techniques.

[Effect of recombinant human osteoprotegerin on osteoclasts stimulated by polyethylene particles].

Objective: To observe the effect of recombinant human osteoprotegerin (rhOPG) on osteoclasts which were stimulated by polyethylene particles and to investigate the feasibility of applying rhOPG for the prosthetic aseptic looseness.

Methods: The osteoclasts were isolated from the long bones of 5 New Zealand rabbits born within 24 hours, weighing 80-100 g, male or female, and were plated into the 24-well coverslips (10 mm x 10 mm) and bone slices (8 mm x 8 mm x 50 microm) at the density of 1 x 10(5)/mL. Based on the different concentration and density of rhOPG and polyethylene particles, the plates of culture were divided into 3 groups: the group with polyethylene particles of 1 x 109/mL (polyethylene group), the group co-cultured with polyethylene particles of 1 x 10(9)/mL and rhOPG of 100 ng/mL (polyethylene/rhOPG group) and the control group.

[Prevention and treatment of early postoperative complications of anterior cervical spinal surgery].

Objective: To study the prevention and management of early complications associated with anterior cervical spinal surgery.

Methods: From November 1998 to December 2006, 363 cases were treated with anterior cervical surgery, including 268 males and 95 females aged 17-79 years. In these cases, 224 suffered from cervical spondylosis, 39 from cervical disc herniation, 87 from cervical trauma, 9 from cervical tumor and 4 from cervical tuberculosis.

[Analysis on length of meridians and ebb-flow of ying-qi based on ancient Shushu].

The theories of Shushu in the literature of Qin and Han periods are reorganized and expositions of foreigner scholars about ancient way of thought are consulted, and compared with the recordation about the ebb-flow of ying-qi and the length of meridians in ancient medical books to probe into the relationship between Shushu and these recordation. It is found that the ebb-flow of ying-qi and the length of meridians are completely conform with the principle of Shushu, i. e.

Study on fibroin heavy chain of the silkworm Bombyx mori by fluorescence in situ hybridization (FISH).

The sericulture industry plays a very important role in our national economy. Silkworm (Bombyx mori) is always regarded as a model animal and biological reactor. There have been detailed studies on the structure, expression and control and molecular evolution of silk genes.

The role of hepatitis B virus x gene in development of primary hepatocellular carcinoma.

Primary hepatocellular carcinoma (HCC) is one of the most common cancers occurring in human, and there is strong epidemiological evidence suggesting that persistent hepatitis B virus (HBV) infection is the most important risk factor for its development.HBx gene was found to be a transactivator recently. Its continuous expression in hepatocytes may transactivate cellular genes which can play a certain role in development of HCC.

High intensity focused ultrasound (HIFU) therapy for local treatment of hepatocellular carcinoma: role of partial rib resection.

Objective: It has long been known that high intensity focused ultrasound (HIFU) can kill tissue through coagulative necrosis. However, it is only in recent years that practical clinical applications are becoming possible. Since the ribs have strong reflections to ultrasonic beams, they may affect the deposition of ultrasound energy, decreasing the efficacy of HIFU treatment and increasing the chance of adverse events when the intra-abdominal tumours concealed by ribs are treated.

Inhibition of hepatitis B virus replication by small interference RNA induces expression of MICA in HepG2.2.15 cells.

Hepatitis B virus (HBV) replicates in most tumor tissues of patients with HBV-associated hepatocellular carcinoma (HCC). In the present study, we have shown that the expression of HBV in the HCC cell lines, HepG2 and Huh7, down-regulated the expression of MHC class I-related molecule A (MICA), a ligand of the NKG2D receptor. Inhibition of HBV expression by small interference RNAs (siRNAs) in HepG2.

[Influence of continuous high-volume hemofiltration on IRAK-4 protein expression in severe acute pancreatitis].

Objective: To investigate the influence of continuous high-volume hemofiltration (CHVHF) on interleukin 1 receptor-associated kinase-4 (IRAK-4) and tumor necrosis factor-alpha (TNF-alpha) levels in patients with severe acute pancreatitis (SAP).

Methods: Forty-one patients with SAP were randomly divided into two groups to receive treatment with CHVHF plus conventional therapy (21 patients) and conventional therapy only (20 patients). Venous blood samples were taken before and 12, 24, and 72 h after the treatment for evaluation of APACHE II scores.

[Changes of blood pressure and pulse rate and the microstructure of celiac ganglion in rabbits with damaged celiac ganglion induced by high intensity focused ultrasound and alcohol].

Objective: To observe the changes of blood pressure (BP), pulse rate (PR) and the microstructure of celiac ganglion (CG) in rabbits with damaged CGs induced by alcohol and high intensity focused ultrasound.

Methods: Fourteen rabbits were randomly divided into two groups. The CGs of the rabbits in group A and group B were damaged by alcohol and high intensity focused ultrasound respectively.

The expression of Bcl-XL, Bcl-XS and p27Kip1 in topotecan-induced apoptosis in hepatoblastoma HepG2 cell line.

Background: To assess the efficacy of topotecan, a topoisomerase I specific inhibitor in S-phase, the reagent-induced apoptosis and cytotoxicity as well as related proteins expression, had been preliminarily investigated in human hepatoblastoma HepG2 cells.

Methods: Microculture tetrazolium assay (MTT), HE staining, transmission electron microscopy (TEM), flow cytometry (FCM), quantitative immunocytochemistry (QI), gene tranfection and RNAi technology were employed to carry out the exploration.

Results: Topotecan could potently kill HepG2 cells via inducing apoptosis and demonstrated strong cytotoxicity in a time, dose-dependent manner with IC50 of about 95 mu g/L.